Difference between revisions of "Team:UiOslo Norway/Experiments/Alkaline Phospatase"

 
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Latest revision as of 12:11, 18 September 2015

Shrimp Alkaline Phosphatase Treatment (Fermentas):

Back to Protocols


Following the provided protocol.

2 μl 10X Reaction Buffer
1 μl shrimp alkaline phosphatase
x μl DNA( 1 μg total concentration)
MilliQ up to 20 μl


Total Reaction of 20 μl


Incubate at 37 °C for 30 minutes
Heat inactivation at 65 °C for 15 minutes.

iGEM UiOslo 2015 is sponsored by: