Difference between revisions of "Team:NAIT Edmonton/Logbook"

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<div class="b-load">
 
<div>
 
<div>
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<img src="http://upload.wikimedia.org/wikipedia/commons/0/0f/Grosser_Panda.JPG" alt=""/>
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<img src="https://static.igem.org/mediawiki/2015/d/d9/NAIT_geekStarter.jpg" title="NAIT at geekStarter"/>
 
<h1>Month 1 - May</h1>
 
<h1>Month 1 - May</h1>
−
<p>I propose we do weekly updates on this log book style thing. I think this is a really neat and intuitive way to display the information that we may find and research each week. For homework, we should try and remember what we did for the past few weeks. We can do a lot of things here including <a href="#">linking</a>. Perhaps a weekly link/update?</p>
+
<p><font color="#8BC5EB"><label class="btn" for="modal-051">Week 1</label></font> | Literature search for the basics of our project.</p>
 +
                                                <p><font color="#88C5EB"><label class="btn" for="modal-052">Week 2</label></font> | Review of the basis of color formation in silver-protein complexes.</p>
 +
                                                <p><font color="#88C5EB"><label class="btn" for="modal-053">Week 3</label></font> | geekStarter and change of course in our project.</p>
 +
                                                <p><font color="#88C5EB"><label class="btn" for ="modal-054">Week 4</label></font> | Offers for sponsorship, getting ourselves established in social media and around NAIT.</p>
  
 
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<img src="http://www.barkteryneexistuje.cz/wp-content/uploads/Cute-Panda-Bears-animals-34915025-2560-1600.jpg" alt="" />
 
<img src="http://www.barkteryneexistuje.cz/wp-content/uploads/Cute-Panda-Bears-animals-34915025-2560-1600.jpg" alt="" />
 
<h1>Month 2 - June</h1>
 
<h1>Month 2 - June</h1>
−
<p><a href="#">Week 1</a> - Secured several sponsorship opportunities this week.</p>
+
<p><font color="#8BC5EB"><label class="btn" for="modal-061">Week 1</label></font> | Secured several sponsorship opportunities this week.</p>
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                         <p><a href="#">Week 2</a> - Click for details etc.</p>
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                         <p><font color="#8BC5EB"><label class="btn" for="modal-062">Week 2</label></font> | Description</p>
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                       <p><a href="#">Week 3</a> - This and this happened this week</p>
+
                       <p><font color="#8BC5EB"><label class="btn" for="modal-063">Week 3</label></font> | Example</p>
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                         <p><a href="#">Week 4</a> - What do you guys think?</p>
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                         <p><font color="#8BC5EB"><label class="btn" for="modal-064">Week 4</label></font> | Example</p>
  
 
</div>
 
</div>
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<img src="http://learn.surbitonhigh.com/pandas2/wp-content/uploads/sites/100/2014/11/cute-panda.jpg" alt="" />
 
<img src="http://learn.surbitonhigh.com/pandas2/wp-content/uploads/sites/100/2014/11/cute-panda.jpg" alt="" />
 
<h1>Month 3 - July</h1>
 
<h1>Month 3 - July</h1>
−
<p>Once upon a time.........</p>
+
                        <p><font color="#8BC5EB"><label class="btn" for="modal-062">Week 1</label></font> | Description</p>
 +
                      <p><font color="#8BC5EB"><label class="btn" for="modal-063">Week 2</label></font> | Example</p>
 +
                        <p><font color="#8BC5EB"><label class="btn" for="modal-064">Week 3</label></font> | Example</p>
 +
                        <p><font color="#8BC5EB"><label class="btn" for="modal-064">Week 4</label></font> | Example</p>
 
</div>
 
</div>
 
<div>
 
<div>
 
<img src="http://www.zooatlanta.org/media/image/panda_cubs2013_140731_meihuan_ZA_7836_600.jpg" alt="" />
 
<img src="http://www.zooatlanta.org/media/image/panda_cubs2013_140731_meihuan_ZA_7836_600.jpg" alt="" />
 
<h1>Month 4 - August</h1>
 
<h1>Month 4 - August</h1>
−
<p>Abbie - Sloth</p>
+
                        <p><font color="#8BC5EB"><label class="btn" for="modal-062">Week 1</label></font> | Description</p>
−
                        <p>Joy - Panda</p>
+
                      <p><font color="#8BC5EB"><label class="btn" for="modal-063">Week 2</label></font> | Example</p>
−
                        <p>Jo - Mantis Shrimp</p>
+
                         <p><font color="#8BC5EB"><label class="btn" for="modal-064">Week 3</label></font> | Example</p>
−
                        <p>David - Wooly Mammoth</p>
+
                         <p><font color="#8BC5EB"><label class="btn" for="modal-064">Week 4</label></font> | Example</p>
−
                         <p>Eduardo - Snow Cougar</p>
+
−
                         <p>Kevin - Mammoth rabbit </p>
+
 
</div>
 
</div>
 
<div>
 
<div>
 
<img src="http://i.huffpost.com/gen/1342502/thumbs/o-PANDA-CUB-900.jpg?6" alt="" />
 
<img src="http://i.huffpost.com/gen/1342502/thumbs/o-PANDA-CUB-900.jpg?6" alt="" />
 
<h1>Crunch Time - September</h1>
 
<h1>Crunch Time - September</h1>
−
<p><a href="#">Week 1</a> - Mainly focused on acquiring sponsorship this week.</p>
+
                        <p><font color="#8BC5EB"><label class="btn" for="modal-062">Week 1</label></font> | Description</p>
−
                         <p><a href="#">Week 2</a></p>
+
                      <p><font color="#8BC5EB"><label class="btn" for="modal-063">Week 2</label></font> | Example</p>
 +
                         <p><font color="#8BC5EB"><label class="btn" for="modal-064">Week 3</label></font> | Example</p>
 +
                        <p><font color="#8BC5EB"><label class="btn" for="modal-064">Week 4</label></font> | Example</p>
  
 
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    <h1>Week 1 - May 4 to 8</h1>
 +
    <p>On week one, we focused on finding literature about the specific chemistry of silver staining. In general, we did not find any literature about the specific, molecular interactions of the silver stain dye with proteins. Many of the articles even stated that the exact mechanism of silver staining remains unknown.</p>
 +
 +
  </div>
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</div>
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<input class="modal-state" id="modal-052" type="checkbox" />
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<div class="modal">
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  <label class="modal__bg" for="modal-052"></label>
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  <div class="modal__inner">
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 +
    <h1>Week 2 - May 11 to 15 </h1>
 +
    <p>This week, we researched on the scientific basis of color. More specifically, we looked into how the interaction between silver, protein and the polyacrylamide gel produces a certain color. We found that the size of silver nano-particles and the refraction of light is how we perceive different colors. </p>
 +
    <br>
 +
    <p> We also tried to look into the electron cloud and how electron excitation produces certain wavelengths of light</p>
 +
 +
  </div>
 +
</div>
 +
 +
<input class="modal-state" id="modal-053" type="checkbox" />
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<div class="modal">
 +
  <label class="modal__bg" for="modal-053"></label>
 +
  <div class="modal__inner">
 +
 +
    <h1>Week 3 - May 18 to 24 </h1>
 +
    <p><img src="https://pbs.twimg.com/profile_images/3000044521/c25c8a20fe16cc64be271c03c0ad6af2_400x400.png" height="50%" width="50%"> Our research continued for the source of color in silver staining.</p>   
 +
    <br>
 +
    <p>We attended the geekStarter workshop hosted by Alberta Innovates at the University of Calgary on Sunday, May 24. We presented questions about iGEM to a panel of experts. We also got one on one "speed-mentoring" with the experts as well as an exclusive group meeting with each of the four experts. Meeting the University of Lethbridge team was also quite cool!</p>
 +
    <br>
 +
    <p> After meeting with the experts, we decided to put the literature review on the back burner so that we could proceed with actual, physical experiments.</p>
 +
 +
  </div>
 +
</div>
 +
 +
<input class="modal-state" id="modal-054" type="checkbox" />
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<div class="modal">
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  <label class="modal__bg" for="modal-054"></label>
 +
  <div class="modal__inner">
 +
 +
    <h1>Week 4 - May 25 to 29 </h1>
 +
    <p>This week, we focused on perfecting our silver staining techniques. We are currently troubleshooting our gels because the stain comes out quite dark and with a lot of background staining.</p>
 +
    <br>
 +
    <p>We also started reaching out to local start-ups and companies looking for sponsorship and further funding.</p>
 +
    <p>Planning our wiki and how we want it organized also took place this week.</p>
 +
 +
 +
  </div>
 +
</div>
 +
 +
<input class="modal-state" id="modal-061" type="checkbox" />
 +
<div class="modal">
 +
  <label class="modal__bg" for="modal-061"></label>
 +
  <div class="modal__inner">
 +
 +
    <h1>Week 1 - June 1 to 5</h1>
 +
    <p>This week, we worked on and finalized the DNA sequences to be sent to IDT.</p>
 +
    <br>
 +
    <p><img src="http://www.bio-rad.com/webroot/web/images/lsr/products/electrophoresis/product_detail/global/lsr_silver_stain_plus_kit.jpg">We also continued perfecting the silver staining techniques outlined by our principal investigator. Unfortunately, we are still experiencing difficulties in background clarity. We tried to switch out the vessels used during staining because we thought that residual silver was affecting our gels. However, even new pyrex containers did not make a difference in our final stained products. We are thinking now that the BioRAD Silver Stain Plus kit that was provided for us contains defective reagents.</p>
 +
 +
  </div>
 +
</div>
 +
 +
<input class="modal-state" id="modal-062" type="checkbox" />
 +
<div class="modal">
 +
  <label class="modal__bg" for="modal-062"></label>
 +
  <div class="modal__inner">
 +
 +
    <h1>Week 2 - June 8 to 12</h1>
 +
    <p></p>
 +
 +
  </div>
 +
</div>
 +
 +
<input class="modal-state" id="modal-063" type="checkbox" />
 +
<div class="modal">
 +
  <label class="modal__bg" for="modal-063"></label>
 +
  <div class="modal__inner">
 +
 +
    <h1>Week 3 - June 15 to 19 </h1>
 +
    <p></p>
 +
 +
  </div>
 +
</div>
 +
 +
<input class="modal-state" id="modal-064" type="checkbox" />
 +
<div class="modal">
 +
  <label class="modal__bg" for="modal-064"></label>
 +
  <div class="modal__inner">
 +
 +
    <h1>Week 4 - June 22 to 26 </h1>
 +
    <p></p>
 +
 +
 +
  </div>
 +
</div>
 +
 +
 
</center>
 
</center>
  

Revision as of 20:47, 9 June 2015

Team NAIT 2015
Our Summer Logbook
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Week 1 - May 4 to 8

On week one, we focused on finding literature about the specific chemistry of silver staining. In general, we did not find any literature about the specific, molecular interactions of the silver stain dye with proteins. Many of the articles even stated that the exact mechanism of silver staining remains unknown.

Week 2 - May 11 to 15

This week, we researched on the scientific basis of color. More specifically, we looked into how the interaction between silver, protein and the polyacrylamide gel produces a certain color. We found that the size of silver nano-particles and the refraction of light is how we perceive different colors.


We also tried to look into the electron cloud and how electron excitation produces certain wavelengths of light

Week 3 - May 18 to 24

Our research continued for the source of color in silver staining.


We attended the geekStarter workshop hosted by Alberta Innovates at the University of Calgary on Sunday, May 24. We presented questions about iGEM to a panel of experts. We also got one on one "speed-mentoring" with the experts as well as an exclusive group meeting with each of the four experts. Meeting the University of Lethbridge team was also quite cool!


After meeting with the experts, we decided to put the literature review on the back burner so that we could proceed with actual, physical experiments.

Week 4 - May 25 to 29

This week, we focused on perfecting our silver staining techniques. We are currently troubleshooting our gels because the stain comes out quite dark and with a lot of background staining.


We also started reaching out to local start-ups and companies looking for sponsorship and further funding.

Planning our wiki and how we want it organized also took place this week.

Week 1 - June 1 to 5

This week, we worked on and finalized the DNA sequences to be sent to IDT.


We also continued perfecting the silver staining techniques outlined by our principal investigator. Unfortunately, we are still experiencing difficulties in background clarity. We tried to switch out the vessels used during staining because we thought that residual silver was affecting our gels. However, even new pyrex containers did not make a difference in our final stained products. We are thinking now that the BioRAD Silver Stain Plus kit that was provided for us contains defective reagents.

Week 2 - June 8 to 12

Week 3 - June 15 to 19

Week 4 - June 22 to 26