Difference between revisions of "Team:UC Davis"

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{{Team:UC Davis }}
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    <title>iGEM at UC Davis</title>
 
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          <a class="navbar-brand" href="#">Team</a>
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     #thumbnail-preview-indicators,
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    #thumbnail-preview-indicators .slides .slide-2,
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          <a class="navbar-brand" href="#">Research</a>
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            <span class="sr-only">Toggle navigation</span>
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}
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          </button>
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}
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          <a class="navbar-brand" href="#">Policy & Practices</a>
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#thumbnail-preview-indicators .slides .slide-3 {
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          <button type="button" class="navbar-toggle collapsed" data-toggle="collapse" data-target="#navbar" aria-expanded="false" aria-controls="navbar">
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  background-image: url(http://i.imgur.com/EfXmCOj.jpg?1);//(http://i.imgur.com/iokYtgR.jpg);
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            <span class="sr-only">Toggle navigation</span>
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}
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            <span class="icon-bar"></span>
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          <a class="navbar-brand" href="#">Get Involved</a>
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#thumbnail-preview-indicators .carousel-indicators li,
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left: 50%;
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/*#top-section {
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margin-left: -687px;
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#top-section-bar {
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background-color: #333;
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height: 1px; //DEFAULT 14 PX
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display: none;//block;
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margin: 0px;
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padding: 0px;
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}
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html, body, .wrapper { /*-- changes default wiki settings --*/
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width: 100%;
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height: 100%;
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background-color: #000000;//#5C8AE6;//#003D99;//transparent;
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font-family: Arial, Helvetica, sans-serif;
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font-weight: normal;
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font-size: 14px;
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color: #414141;
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margin-left: auto;
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margin-right: auto;
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margin-top: 0px;
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</style>
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<body>
 +
<div style="background-color:#fff">
 +
<!--<div class="container-fluid" id="allHome">-->
 +
        <!--<div class = "nav nav-justified " role = "navigation">-->
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<!-- UNCOMMENT TO REMOVE FULL WIDTH NAV BAR <div class="container-fluid"> -->
 +
           
 +
            <!--navbar -->  
 +
       
 +
            <!--<nav class="navbar nav-justified" role="navigation" id='nav'>
 +
                <div class="btn-group  btn-group btn-group-justified">
 +
                <ul class="nav  navbar-nav" role="navigation" id='nav'>
 +
                    <li class = "active"><div class="btn btn-lg" id="home">Home</div></li>
 +
                    <li><div class="btn btn-lg" id="team">Team</div></li>
 +
 
 +
       
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 +
                   
 +
                    <li><div class="btn btn-lg" id="notebook">Notebook</div></li>
 +
                    <li><div class="btn btn-lg" id="achieve">Software Achievements</div></li>
 +
                    <li><div class="btn btn-lg" id="attributions">Attributions</div></li>
 +
                    <li><div class="btn btn-lg" id="test">Tester</div></li>
 +
                    </ul>
 +
                   
 +
              </div>
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 +
               
 +
               
 +
        </nav>-->
 +
        <!-- FOR IMAGE! LOGOS IGEM & UCSD --><!--<nav class="navbar nav-justified" role="navigation" id='nav'>
 +
                <div class="btn-group  btn-group btn-group-justified" >
 +
                <div class="btn btn-lg " id="avant">UC Davis</div>-->
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                <!--<font size = 4px>UC Davis</font>-->
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              <!--<div class="btn-group  btn-group btn-group-justified">
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                <img src = "http://i.imgur.com/mGHw11P.png?1">
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              </div>
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                </div>
 +
          </nav>-->
 +
        <nav class="navbar nav-justified" role="navigation" id='nav'>
 +
                <div class="btn-group  btn-group btn-group-justified">
 +
                    <div class="btn btn-lg " id="home">Home</div>
 +
                    <div class="btn btn-lg " id="team">Team</div>
 +
                    <div class="btn btn-lg " id="project">Project</div>
 +
                    <div class="btn btn-lg " id="doc">Policy & Practices</div>
 +
                    <div class="btn btn-lg" id="notebook">Notebook</div>
 +
                    <div class="btn btn-lg" id="attributions">Sponsors</div>
 +
                    <div class="btn btn-lg" id="achieve">Medals</div>
 +
                    <div class="btn btn-lg" id="logo"><a href = "https://igem.org/Main_Page"><img src = "https://static.igem.org/mediawiki/2014/c/c0/IGEM_logo_spinning_nevada_igem_2014.gif" height="80" width="160"></a></div>
 +
                   
 +
              </div>
 +
               
 +
            </nav>
 +
     
 +
         <!-- container fluid END </div> -->
 +
 +
<!--slider-->
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 +
<div id="thumbnail-preview-indicators" class="carousel slide" data-ride="carousel">
 
            
 
            
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          <!--<ol class="carousel-indicators">
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            <li data-target="#thumbnail-preview-indicators" data-slide-to="0" class="active">
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     </nav>
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              <div class="thumbnail">
 +
                <img class="img-responsive" src="http://www.blueoceanart.com/images/upload/AOP_KelpForest1_JimHellemn.jpg">
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              </div>
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            <li data-target="#thumbnail-preview-indicators" data-slide-to="1">
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            </li>
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          </ol>-->
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          <div class="carousel-inner">
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            <div class="item slides active">
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              <div class="slide-1"></div>
 +
              <div class="container">
 +
                <div class="carousel-caption">
 +
                  <h1 style="font-size:500%"><font color =  #ffffff >UC Davis Aggies</font></h1>
 +
                </div>
 +
              </div>
 +
            </div>
 +
 
 +
            <div class="item slides">
 +
              <div class="slide-2"></div>
 +
              <div class="container">
 +
                <div class="carousel-caption">
 +
                  <h1><font color = "#000000">SBiDer</font></h1>
 +
                  <p><a class="btn btn-lg btn-primary" href="#" role="button">Take me to the app!</a></p>         
 +
                </div>
 +
              </div>
 +
            </div>
 +
            <div class="item slides">
 +
              <div class="slide-3"></div>
 +
              <div class="container">
 +
                <div class="carousel-caption">
 +
                  <br><br><h1><font color = "gold">The Notebook</font></h1>
 +
                  <p><a class="btn btn-lg btn-primary" href="https://2014.igem.org/Team:UCSD_Software/Notebook" role="button">View the Notebook</a></p>
 +
                </div>
 +
              </div>
 +
            </div>
 +
          </div>
 +
          <a class="left carousel-control" href="#thumbnail-preview-indicators" role="button" data-slide="prev"><span class="glyphicon glyphicon-chevron-left"></span></a>
 +
          <a class="right carousel-control" href="#thumbnail-preview-indicators" role="button" data-slide="next"><span class="glyphicon glyphicon-chevron-right"></span></a>
 +
       </div>  
 +
 
 +
 
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 +
<!--slider end-->
 +
 
 +
<div class = "neighborhood-guides">
 +
        <div class = "container">
 +
<tr><td colspan="3"><div class = "well" style="margin-bottom:50px"><p> <strong><center>Producing a Novel Antimicrobial Surface-Binding Peptide Using an Improved T7 Expression System</center></strong><p>
 +
Biofilm formation on surfaces is an issue in the medical field, naval industry, and other areas. We developed an anti-fouling peptide with two modular components: a mussel adhesion protein (MAP) anchor and LL-37, an antimicrobial peptide. MAPs can selectively attach to metal and organic surfaces via L-3,5-dihydroxyphenylalanine (L-DOPA), a nonstandard amino acid that was incorporated using a genetically recoded organism (GRO).  Because this peptide is toxic to the GRO in which it is produced, we designed a better controlled inducible system that limits basal expression. This was achieved through a novel T7 riboregulation system that controls expression at both the transcriptional and translational levels.  This improved system is a precise synthetic switch for the expression of cytotoxic substances in the already robust T7 system. Lastly, the antimicrobial surface-binding peptide was assayed for functionality.
 +
</p></div></td>
 +
</tr>
 +
 
 +
 
 +
<tr><td colspan="3"><div class = "well" style="margin-bottom:100px"><a href="https://2014.igem.org/Team:Yale/Project"><img src="
 +
https://static.igem.org/mediawiki/2014/f/f8/Yale-Overview.png"  width="1100"></a></td></tr>
 +
 
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<tr><td colspan="3"><a href="https://2014.igem.org/Team:Yale/Team"><img src="https://static.igem.org/mediawiki/2014/5/50/Yale-Team.png"  width="1100"></a></td></tr>
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Revision as of 20:30, 9 September 2015


Producing a Novel Antimicrobial Surface-Binding Peptide Using an Improved T7 Expression System

Biofilm formation on surfaces is an issue in the medical field, naval industry, and other areas. We developed an anti-fouling peptide with two modular components: a mussel adhesion protein (MAP) anchor and LL-37, an antimicrobial peptide. MAPs can selectively attach to metal and organic surfaces via L-3,5-dihydroxyphenylalanine (L-DOPA), a nonstandard amino acid that was incorporated using a genetically recoded organism (GRO). Because this peptide is toxic to the GRO in which it is produced, we designed a better controlled inducible system that limits basal expression. This was achieved through a novel T7 riboregulation system that controls expression at both the transcriptional and translational levels. This improved system is a precise synthetic switch for the expression of cytotoxic substances in the already robust T7 system. Lastly, the antimicrobial surface-binding peptide was assayed for functionality.

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Copyright (c) 2014 Yale IGEM