Difference between revisions of "Team:Valencia UPV/Notebook"

Line 18: Line 18:
  
 
<!-- Main -->
 
<!-- Main -->
<div class="row">
+
<section class="box special">
<div class="12u">
+
<header class="major">
 +
<h2>Notebook
 +
<br />
 +
</h2>
 +
</header>
 +
<div class="row">
 +
<div class="12u">
 +
<section class="box">
 +
 +
<h3 style="color:green">5 June 2015</h3>
  
<!-- Text -->
+
<p>We had 2 cultures from the last day, corresponding to other 2 colonies of ligation. </p>
<section class="box">
+
<h3>Text</h3>
+
<p>This is <b>bold</b> and this is <strong>strong</strong>. This is <i>italic</i> and this is <em>emphasized</em>.
+
This is <sup>superscript</sup> text and this is <sub>subscript</sub> text.
+
This is <u>underlined</u> and this is code: <code>for (;;) { ... }</code>. Finally, <a href="#">this is a link</a>.</p>
+
  
<hr />
+
<p><i>Agrobacterium</i> culture of promoter less: Luciferase + Renilla </p>
  
<header>
+
<p>Minipreps</p>
<h3>Heading with a Subtitle</h3>
+
<p>Lorem ipsum dolor sit amet nullam id egestas urna aliquam</p>
+
</header>
+
<p>Nunc lacinia ante nunc ac lobortis. Interdum adipiscing gravida odio porttitor sem non mi integer non faucibus ornare mi ut ante amet placerat aliquet. Volutpat eu sed ante lacinia sapien lorem accumsan varius montes viverra nibh in adipiscing blandit tempus accumsan.</p>
+
<header>
+
<h4>Heading with a Subtitle</h4>
+
<p>Lorem ipsum dolor sit amet nullam id egestas urna aliquam</p>
+
</header>
+
<p>Nunc lacinia ante nunc ac lobortis. Interdum adipiscing gravida odio porttitor sem non mi integer non faucibus ornare mi ut ante amet placerat aliquet. Volutpat eu sed ante lacinia sapien lorem accumsan varius montes viverra nibh in adipiscing blandit tempus accumsan.</p>
+
  
<hr />
+
<p>Digestion with BamHI and EcoRV</p>
  
<h2>Heading Level 2</h2>
+
<div class="table-wrapper"><table class="alt">
<h3>Heading Level 3</h3>
+
<h4>Heading Level 4</h4>
+
<h5>Heading Level 5</h5>
+
<h6>Heading Level 6</h6>
+
  
<hr />
+
<tr><td>PIF6 + PhyB; &Omega;1</td><td>Etr8 CMV+Bxb1_T35S; &alpha;1</td></tr>
  
</section>
+
<tr><td>1µL (GB892) PIF; &alpha;1</td><td>1µL 1097 (Etr8 CMV) pUPD2</td></tr>
  
</div>
+
<tr><td>1µL (GB88E) PhyB; &alpha;2</td><td>1µL Bxb1; pUPD2</td></tr>
</div>
+
 
 +
<tr><td>1µL &Omega;1 </td><td>1µL Tnos PuPD</td></tr>
 +
 
 +
<tr><td>1.2µL Buffer ligase</td><td>1µL &alpha;1</td></tr>
 +
 
 +
<tr><td>1µL Bsmb1</td><td>5.8µL H<sub>2</sub>O</td></tr>
 +
 
 +
<tr><td>6.8µL H<sub>2</sub>O</td><td></td></tr>
 +
 
 +
</div></table>
 +
 
 +
</section>
 +
</div>
 +
</div>
 +
</section>
 
</html>
 
</html>
 
{{:Team:Valencia_UPV/Templates:footerUPV}}
 
{{:Team:Valencia_UPV/Templates:footerUPV}}

Revision as of 13:25, 10 September 2015

Valencia UPV iGEM 2015

Notebook

5 June 2015

We had 2 cultures from the last day, corresponding to other 2 colonies of ligation.

Agrobacterium culture of promoter less: Luciferase + Renilla

Minipreps

Digestion with BamHI and EcoRV

PIF6 + PhyB; Ω1Etr8 CMV+Bxb1_T35S; α1
1µL (GB892) PIF; α11µL 1097 (Etr8 CMV) pUPD2
1µL (GB88E) PhyB; α21µL Bxb1; pUPD2
1µL Ω1 1µL Tnos PuPD
1.2µL Buffer ligase1µL α1
1µL Bsmb15.8µL H2O
6.8µL H2O