Difference between revisions of "Team:Technion Israel/Lab Notebook/Protocols"
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<div class="four_columns"> | <div class="four_columns"> | ||
<div id="pic_cloning"> | <div id="pic_cloning"> | ||
| − | + | <img src="https://static.igem.org/mediawiki/2015/4/47/DNA.png" alt="DNA and Cloning" width="900px" height="100px"> | |
| − | + | </div> | |
| − | + | <div class="wrapper"> | |
| − | + | <div class="cloning_left"> | |
<ul> | <ul> | ||
| − | <li> | + | <li style="list-style-type: none;"> |
<h2>Gel Extraction</h2> | <h2>Gel Extraction</h2> | ||
</li> | </li> | ||
| − | <li> | + | <li style="list-style-type: none;"> |
<h2>Sigma-Aldrich Miniprep</h2> | <h2>Sigma-Aldrich Miniprep</h2> | ||
</li> | </li> | ||
| − | <li> | + | <li style="list-style-type: none;"> |
<h2>Phusion PCR</h2> | <h2>Phusion PCR</h2> | ||
</li> | </li> | ||
| − | <li> | + | <li style="list-style-type: none;"> |
<h2>Electroporation</h2> | <h2>Electroporation</h2> | ||
<ol> | <ol> | ||
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<div class="cloning_right"> | <div class="cloning_right"> | ||
<ul> | <ul> | ||
| − | <li> | + | <li style="list-style-type: none;"> |
<h2>Transformation</h2> | <h2>Transformation</h2> | ||
<ol> | <ol> | ||
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</ol> | </ol> | ||
</li> | </li> | ||
| − | <li> | + | <li style="list-style-type: none;"> |
<h2>Colony PCR</h2> | <h2>Colony PCR</h2> | ||
<ol><a href="https://static.igem.org/mediawiki/2015/b/bf/Technion_Israel_2015_Colony_PCR_for_B.Subtilis_.pdf"><h3><i>B. Subtilis</i></h3></a> | <ol><a href="https://static.igem.org/mediawiki/2015/b/bf/Technion_Israel_2015_Colony_PCR_for_B.Subtilis_.pdf"><h3><i>B. Subtilis</i></h3></a> | ||
</ol> | </ol> | ||
</li> | </li> | ||
| − | <li> | + | <li style="list-style-type: none;"> |
<h2>Preparing Competent <i>E.coli</i></h2> | <h2>Preparing Competent <i>E.coli</i></h2> | ||
</li> | </li> | ||
| − | <li> | + | <li style="list-style-type: none;"> |
<h2>DNA Gel Electrophoresis</h2> | <h2>DNA Gel Electrophoresis</h2> | ||
</li> | </li> | ||
| − | <li> | + | <li style="list-style-type: none;"> |
<h2>Preparation of DNA for Sequencing</h2> | <h2>Preparation of DNA for Sequencing</h2> | ||
</li> | </li> | ||
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<div class="cloning_left"> | <div class="cloning_left"> | ||
<ul> | <ul> | ||
| − | <li><a href="https://static.igem.org/mediawiki/2015/4/4b/Technion_Israel_2015_LysisMethodComparison.pdf" target="_blank"><h2>Comparison of Lysis Procedures</h2></a> | + | <li style="list-style-type: none;"><a href="https://static.igem.org/mediawiki/2015/4/4b/Technion_Israel_2015_LysisMethodComparison.pdf" target="_blank"><h2>Comparison of Lysis Procedures</h2></a> |
| − | </li><li><a href="https://static.igem.org/mediawiki/2015/e/e2/Technion_Israel_2015_protocols_B.subtilis-mCherry-secretion.pdf" target="_blank"><h2>Bacillus Secretion Verification</h2></a></li> | + | </li><li style="list-style-type: none;"><a href="https://static.igem.org/mediawiki/2015/e/e2/Technion_Israel_2015_protocols_B.subtilis-mCherry-secretion.pdf" target="_blank"><h2>Bacillus Secretion Verification</h2></a></li> |
| + | </ul> | ||
</div> | </div> | ||
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</div> | </div> | ||
</div> | </div> | ||
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<li> | <li> | ||
<div class="slides"> | <div class="slides"> | ||
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<div id="pic_misc"> | <div id="pic_misc"> | ||
<img style="border-top: 5px solid black; border-bottom: 5px solid black" src="https://static.igem.org/mediawiki/2015/7/7c/Technion_Israel_2015_Protocols_Misc.jpg" alt="Miscellaneous" width="900px" height="100px"></a> | <img style="border-top: 5px solid black; border-bottom: 5px solid black" src="https://static.igem.org/mediawiki/2015/7/7c/Technion_Israel_2015_Protocols_Misc.jpg" alt="Miscellaneous" width="900px" height="100px"></a> | ||
</div> | </div> | ||
<div class="cloning_left"> | <div class="cloning_left"> | ||
| − | <ul> | + | <ul style="list-style-type: none;"> |
| − | <li><a href="https://static.igem.org/mediawiki/2015/b/bb/Technion_iGEM_2015_Primer_dilution_protocol.pdf" target="_blank"><h2>Primer Dilution</h2></a></li> | + | <li style="list-style-type: none;"><a href="https://static.igem.org/mediawiki/2015/b/bb/Technion_iGEM_2015_Primer_dilution_protocol.pdf" target="_blank"><h2>Primer Dilution</h2></a></li> |
</ul> | </ul> | ||
| + | </div> | ||
| + | <div class="cloning_right"></div> | ||
</div> | </div> | ||
</div> | </div> | ||
Revision as of 15:41, 11 September 2015
Protocols
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-
Gel Extraction
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Sigma-Aldrich Miniprep
-
Phusion PCR
-
Electroporation
E.coli
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Transformation
E.coli
B.Subtilis
-
Colony PCR
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Preparing Competent E.coli
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DNA Gel Electrophoresis
-
Preparation of DNA for Sequencing
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