Difference between revisions of "Team:WHU-China/DentalCP"
Line 345: | Line 345: | ||
<h2>How to control the population of S.mutans</h2> | <h2>How to control the population of S.mutans</h2> | ||
− | <span style="margin-bottom: | + | <span style="margin-bottom:35%">Antimicrobial peptides are recently regarded as a promising choice to kill microbes due to their strong microbicidal effects and their unlikeliness of triggering resistance in microorganisms. Since there are also various kinds of probiotics living in our mouth besides oral pathogens, and since antimicrobial peptides have effect on general kinds of microorganisms, it’s more wise to modify the antimicrobial peptide into a targeted one by adding a S.mutans targeted sequence on the N-terminal, so that only S.mutans can be killed. Thus we design two fused peptides, CAP-glyglygly-Bac8c and CAP-glyglygly-APP2, to targetedly inhibit the growth of S.mutans in oral cavity, where CAP is an optimized peptide that can specifically bind to the comD receptor on the surface of S.mutans, while Bac8c and APP2 are two kinds of peptides that assume strong bactericidal effect in acidic environment. |
<br /> | <br /> | ||
<img src="https://static.igem.org/mediawiki/2015/6/63/WHU-China_KJT.png" width="400"></img> | <img src="https://static.igem.org/mediawiki/2015/6/63/WHU-China_KJT.png" width="400"></img> | ||
Line 356: | Line 356: | ||
<h2>How does the whole system work</h2> | <h2>How does the whole system work</h2> | ||
− | <span style="margin-bottom: | + | <span style="margin-bottom:50%">When the concentration of S.mutans in mouth increases, the pH of the micro-environment decreases as well, and reaching to certain level it will activate promoter AsR and induce the reaction downstream in different intensity. The signal intensity will be processed by our criticality detector and an final output signal containing the information whether the antimicrobial peptide gene should or should not be expressed will transfer to the peptide expression circuit to control the expression of antimicrobial peptide. Only when the concentration of S.mutans reaches certain level, can the peptide expression circuit be activated, so that the targeted antimicrobial peptide can inhibit the growth of S.mutans, reducing its population to a lower and healthy level. |
<br /> | <br /> | ||
<img src="https://static.igem.org/mediawiki/2015/b/b3/WHU-China_KJT3.png" width="600"></img></span> | <img src="https://static.igem.org/mediawiki/2015/b/b3/WHU-China_KJT3.png" width="600"></img></span> |
Revision as of 17:33, 11 September 2015