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− | <!-- NO EXPORT START | + | <!-- NO EXPORT START |
| <html> | | <html> |
| <head> | | <head> |
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| ; (function(a){a.fn.rwdImageMaps = function(){var c = this; var b = function(){c.each(function(){if (typeof (a(this).attr("usemap")) == "undefined"){return}var e = this, d = a(e); a("<img />").load(function(){var g = "width", m = "height", n = d.attr(g), j = d.attr(m); if (!n || !j){var o = new Image(); o.src = d.attr("src"); if (!n){n = o.width}if (!j){j = o.height}}var f = d.width() / 100, k = d.height() / 100, i = d.attr("usemap").replace("#", ""), l = "coords"; a('map[name="' + i + '"]').find("area").each(function(){var r = a(this); if (!r.data(l)){r.data(l, r.attr(l))}var q = r.data(l).split(","), p = new Array(q.length); for (var h = 0; h < p.length; ++h){if (h % 2 === 0){p[h] = parseInt(((q[h] / n) * 100) * f)} else{p[h] = parseInt(((q[h] / j) * 100) * k)}}r.attr(l, p.toString())})}).attr("src", d.attr("src"))})}; a(window).resize(b).trigger("resize"); return this}})(jQuery); | | ; (function(a){a.fn.rwdImageMaps = function(){var c = this; var b = function(){c.each(function(){if (typeof (a(this).attr("usemap")) == "undefined"){return}var e = this, d = a(e); a("<img />").load(function(){var g = "width", m = "height", n = d.attr(g), j = d.attr(m); if (!n || !j){var o = new Image(); o.src = d.attr("src"); if (!n){n = o.width}if (!j){j = o.height}}var f = d.width() / 100, k = d.height() / 100, i = d.attr("usemap").replace("#", ""), l = "coords"; a('map[name="' + i + '"]').find("area").each(function(){var r = a(this); if (!r.data(l)){r.data(l, r.attr(l))}var q = r.data(l).split(","), p = new Array(q.length); for (var h = 0; h < p.length; ++h){if (h % 2 === 0){p[h] = parseInt(((q[h] / n) * 100) * f)} else{p[h] = parseInt(((q[h] / j) * 100) * k)}}r.attr(l, p.toString())})}).attr("src", d.attr("src"))})}; a(window).resize(b).trigger("resize"); return this}})(jQuery); |
| </script> | | </script> |
− | <img alt="Project overview" src="[[File:Description-overview.png|caption]]" usemap="#overview" /> | + | <img alt="Project overview" src="https://static.igem.org/mediawiki/2015/0/04/Description-overview.png" usemap="#overview" /> |
| <map name="overview"> | | <map name="overview"> |
| <area shape="circle" coords="120,178,101" alt="Overview" href="#background" /> | | <area shape="circle" coords="120,178,101" alt="Overview" href="#background" /> |
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| </div> | | </div> |
| <div class="half last"> | | <div class="half last"> |
− | <img class="" alt="Genetic module and parts in legos" src="https://static.igem.org/mediawiki/2015/8/83/Description-genetic_module_parts_legos.png" /> | + | <img class="" alt="Genetic module and parts in legos" src="https://static.igem.org/mediawiki/2015/f/f2/Description-genetic_module_parts_legos.png" /> |
| </div> | | </div> |
| </div> | | </div> |
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| <div class="half"> | | <div class="half"> |
| <h3>E. Coli 1 Poblation: “Lactadora”</h3> | | <h3>E. Coli 1 Poblation: “Lactadora”</h3> |
− | <img class="" alt="Lactadora" src="https://static.igem.org/mediawiki/2015/8/83/Lego_description-bacteria_lego.png" /> | + | <img class="" alt="Lactadora" src="https://static.igem.org/mediawiki/2015/0/0b/Lego_description-bacteria_lego.png" /> |
| <p>In our project we used two bacterias « Escherichia coli »: name and first name of the bacteria.<br>This population are responsible for lactate production.</p> | | <p>In our project we used two bacterias « Escherichia coli »: name and first name of the bacteria.<br>This population are responsible for lactate production.</p> |
| </div> | | </div> |
| <div class="half last"> | | <div class="half last"> |
| <h3>E. Coli 2 Poblation: “PLAdora”</h3> | | <h3>E. Coli 2 Poblation: “PLAdora”</h3> |
− | <img class="" alt="PLAdora" src="https://static.igem.org/mediawiki/2015/8/83/Lego_description-bacteria_lego.png" /> | + | <img class="" alt="PLAdora" src="https://static.igem.org/mediawiki/2015/0/0b/Lego_description-bacteria_lego.png" /> |
| <p>« Escherichia coli »: name and first name of the bacteria. « 1 » y « 2 » only mean that these bacteria, in this process, are responsible for different functions (but they belong the same bacteria strain).<br>This population are responsible for process the lactate into PLA.</p> | | <p>« Escherichia coli »: name and first name of the bacteria. « 1 » y « 2 » only mean that these bacteria, in this process, are responsible for different functions (but they belong the same bacteria strain).<br>This population are responsible for process the lactate into PLA.</p> |
| </div> | | </div> |
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| <div class="division"> | | <div class="division"> |
| <div class="two_third"> | | <div class="two_third"> |
− | <img class="" alt="LDH" src="https://static.igem.org/mediawiki/2015/8/83/Lego_description-LDH.png" /> | + | <img class="" alt="LDH" src="https://static.igem.org/mediawiki/2015/7/7f/Lego_description-LDH.png" /> |
| </div> | | </div> |
| <div class="one_third last"> | | <div class="one_third last"> |
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| </div> | | </div> |
| </div> | | </div> |
− | <img class="" alt="Scheme" src="https://static.igem.org/mediawiki/2015/8/83/Lego_description-esquema.png" /> | + | <img class="" alt="Scheme" src="https://static.igem.org/mediawiki/2015/6/64/Lego_description-esquema.png" /> |
| <div id="esquema_descripcion" class="division"> | | <div id="esquema_descripcion" class="division"> |
| <div class="one_third"> | | <div class="one_third"> |
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| <div class="division"> | | <div class="division"> |
| <div class="two_third"> | | <div class="two_third"> |
− | <img class="" alt="Lactate" src="https://static.igem.org/mediawiki/2015/8/83/Lego_description-lactate.png" /> | + | <img class="" alt="Lactate" src="https://static.igem.org/mediawiki/2015/9/90/Lego_description-lactate.png" /> |
| </div> | | </div> |
| <div class="one_third last"> | | <div class="one_third last"> |
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| </div> | | </div> |
| </div> | | </div> |
− | <img class="" alt="Lactate" src="https://static.igem.org/mediawiki/2015/8/83/Lego_description-lactate_populations.png" /> | + | <img class="" alt="Lactate" src="https://static.igem.org/mediawiki/2015/b/b6/Lego_description-lactate_populations.png" /> |
| <div class="division"> | | <div class="division"> |
| <div class="half"> | | <div class="half"> |
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| <div class="division"> | | <div class="division"> |
| <div class="two_third"> | | <div class="two_third"> |
− | <img class="" alt="PhaC" src="https://static.igem.org/mediawiki/2015/8/83/Lego_description-phac.png" /> | + | <img class="" alt="PhaC" src="https://static.igem.org/mediawiki/2015/3/3f/Lego_description-phac.png" /> |
| </div> | | </div> |
| <div class="one_third last"> | | <div class="one_third last"> |
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| </div> | | </div> |
| </div> | | </div> |
− | <img class="" alt="PLA" src="https://static.igem.org/mediawiki/2015/8/83/Lego_description-lactato_pla.png" /> | + | <img class="" alt="PLA" src="https://static.igem.org/mediawiki/2015/0/0d/Lego_description-lactato_pla.png" /> |
| <div id="esquema_descripcion" class="division"> | | <div id="esquema_descripcion" class="division"> |
| <div class="one_third"> | | <div class="one_third"> |
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| </article> | | </article> |
| <article> | | <article> |
− | <img class="" alt="Lactate to PLA" src="https://static.igem.org/mediawiki/2015/8/83/Lego_description-sumpup_lactate_to_pla.png" /> | + | <img class="" alt="Lactate to PLA" src="https://static.igem.org/mediawiki/2015/3/38/Lego_description-sumpup_lactate_to_pla.png" /> |
| <div class="division"> | | <div class="division"> |
| <div class="half"> | | <div class="half"> |
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| </article> | | </article> |
| <article> | | <article> |
− | <img class="" alt="PLA ready to export" src="https://static.igem.org/mediawiki/2015/8/83/Lego_description-pla_export.png" /> | + | <img class="" alt="PLA ready to export" src="https://static.igem.org/mediawiki/2015/f/f3/Lego_description-pla_export.png" /> |
| <p class="harto_margen">The hybrid protein sticks with PLA and the cell exports it into the extra-cellular medium, ready to be extracted and purified.</p> | | <p class="harto_margen">The hybrid protein sticks with PLA and the cell exports it into the extra-cellular medium, ready to be extracted and purified.</p> |
| </article> | | </article> |
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| </div> | | </div> |
| </div> | | </div> |
− | <img class="" alt="E1 E2 population" src="https://static.igem.org/mediawiki/2015/8/83/Lego_description-important_modules.png" /> | + | <img class="" alt="E1 E2 population" src="https://static.igem.org/mediawiki/2015/4/49/Lego_description-important_modules.png" /> |
| <div class="division"> | | <div class="division"> |
| <div class="half"> | | <div class="half"> |
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| <article> | | <article> |
| <h3>Regulation of lactate production in E.coli 1</h3> | | <h3>Regulation of lactate production in E.coli 1</h3> |
− | <img class="" alt="Regulation E1" src="https://static.igem.org/mediawiki/2015/8/83/Lego_description-regulation_E1.png" /> | + | <img class="" alt="Regulation E1" src="https://static.igem.org/mediawiki/2015/c/cb/Lego_description-regulation_E1.png" /> |
| <p>When being outside the cells, lactate acidifies the medium, which is dangerous for the bacteria. That’s why a pH-sensing module has been built to detect pH levels and produce the TetR protein when the pH is lower than 5,5.</p> | | <p>When being outside the cells, lactate acidifies the medium, which is dangerous for the bacteria. That’s why a pH-sensing module has been built to detect pH levels and produce the TetR protein when the pH is lower than 5,5.</p> |
| <div class="division"> | | <div class="division"> |
| <div class="two_third"> | | <div class="two_third"> |
− | <img class="" alt="Regulation E1" src="https://static.igem.org/mediawiki/2015/8/83/Lego_description-ph_low.png" /> | + | <img class="" alt="Regulation E1" src="https://static.igem.org/mediawiki/2015/9/92/Lego_description-ph_low.png" /> |
| </div> | | </div> |
| <div class="one_third last"> | | <div class="one_third last"> |
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| <div class="division"> | | <div class="division"> |
| <div class="two_third"> | | <div class="two_third"> |
− | <img class="" alt="Regulation E1" src="https://static.igem.org/mediawiki/2015/8/83/Lego_description-ph_increase.png" /> | + | <img class="" alt="Regulation E1" src="https://static.igem.org/mediawiki/2015/3/39/Lego_description-ph_increase.png" /> |
| </div> | | </div> |
| <div class="one_third last"> | | <div class="one_third last"> |
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| <article> | | <article> |
| <h3>Communication between E.coli 1 and E.coli 2</h3> | | <h3>Communication between E.coli 1 and E.coli 2</h3> |
− | <img class="" alt="Regulation E1" src="https://static.igem.org/mediawiki/2015/8/83/Lego_description-communication.png" /> | + | <img class="" alt="Regulation E1" src="https://static.igem.org/mediawiki/2015/a/af/Lego_description-communication.png" /> |
| <p>In fact, the module that produces lactate not only produces it but is also responsible for the synthesis of a small molecule called HSL (HomoSeryl-Lactone) . This molecule is exported into the medium and is able to enter the second bacteria, being responsible for communication between E.coli 1 and 2.</p> | | <p>In fact, the module that produces lactate not only produces it but is also responsible for the synthesis of a small molecule called HSL (HomoSeryl-Lactone) . This molecule is exported into the medium and is able to enter the second bacteria, being responsible for communication between E.coli 1 and 2.</p> |
| <div class="division"> | | <div class="division"> |
| <div class="two_third"> | | <div class="two_third"> |
− | <img class="" alt="Regulation E2" src="https://static.igem.org/mediawiki/2015/8/83/Lego_description-hsl.png" /> | + | <img class="" alt="Regulation E2" src="https://static.igem.org/mediawiki/2015/3/31/Lego_description-hsl.png" /> |
| </div> | | </div> |
| <div class="one_third last"> | | <div class="one_third last"> |
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| <div class="division"> | | <div class="division"> |
| <div class="two_third"> | | <div class="two_third"> |
− | <img class="" alt="Regulation E2" src="https://static.igem.org/mediawiki/2015/8/83/Lego_description-luxr.png" /> | + | <img class="" alt="Regulation E2" src="https://static.igem.org/mediawiki/2015/3/33/Lego_description-luxr.png" /> |
| </div> | | </div> |
| <div class="one_third last"> | | <div class="one_third last"> |
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| <article> | | <article> |
| <h3>Communication between E.coli 1 and E.coli 2</h3> | | <h3>Communication between E.coli 1 and E.coli 2</h3> |
− | <img class="" alt="Communication between" src="https://static.igem.org/mediawiki/2015/8/83/Lego_description-between.png" /> | + | <img class="" alt="Communication between" src="https://static.igem.org/mediawiki/2015/c/c8/Lego_description-between.png" /> |
| <p>The concerted action of LuxR with HSL activates the PLA production and makes possible its exportation. This activation is possible only if there is enough lactate in the medium (since it is the same module that produces lactate and HSL). This way, E.coli 1 leads the production, telling to E.coli 2 when the PLA synthesis can begin). That’s why it is said that HSL is a communication molecule.</p> | | <p>The concerted action of LuxR with HSL activates the PLA production and makes possible its exportation. This activation is possible only if there is enough lactate in the medium (since it is the same module that produces lactate and HSL). This way, E.coli 1 leads the production, telling to E.coli 2 when the PLA synthesis can begin). That’s why it is said that HSL is a communication molecule.</p> |
− | <img class="" alt="Communication global" src="https://static.igem.org/mediawiki/2015/8/83/Lego_description-global.png" /> | + | <img class="" alt="Communication global" src="https://static.igem.org/mediawiki/2015/a/a0/Lego_description-global.png" /> |
| <p>The concerted action of LuxR with HSL activates the PLA production and makes possible its exportation. This activation is possible only if there is enough lactate in the medium (since it is the same module that produces lactate and HSL). This way, E.coli 1 leads the production, telling to E.coli 2 when the PLA synthesis can begin). That’s why it is said that HSL is a communication molecule.</p> | | <p>The concerted action of LuxR with HSL activates the PLA production and makes possible its exportation. This activation is possible only if there is enough lactate in the medium (since it is the same module that produces lactate and HSL). This way, E.coli 1 leads the production, telling to E.coli 2 when the PLA synthesis can begin). That’s why it is said that HSL is a communication molecule.</p> |
| </article> | | </article> |
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| <div class="division"> | | <div class="division"> |
| <div class="half"> | | <div class="half"> |
− | <img class="" alt="Process" src="https://static.igem.org/mediawiki/2015/8/83/Main_goal-process.png" /> | + | <img class="" alt="Process" src="https://static.igem.org/mediawiki/2015/8/82/Main_goal-process.png" /> |
| </div> | | </div> |
| <div class="half last"> | | <div class="half last"> |