Difference between revisions of "Template:SJTU-BioX-Shanghai/Notebook/Transformation/w10"
(→pdark-GFP electro-transformation) |
(→first desalination test of pcpcG2-HR) |
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===first desalination test of pcpcG2-HR=== | ===first desalination test of pcpcG2-HR=== | ||
| + | '''on 7th, Sep''' | ||
| + | |||
| + | Inoculated 15 wildtype & 15 pcpcG2-HR (use pcpcG2-HR from colony 2) at 10pm | ||
| + | |||
| + | '''on 9th, Sep''' | ||
| + | |||
| + | Induced with green light at 10pm, added 22g/L NaCl & retinene, then took the first 6 samples (3 parallel samples for each) | ||
| + | |||
| + | '''on 10th, Sep''' | ||
| + | |||
| + | cultivated with red light at 10am, then took the second 6 samples, | ||
| + | |||
| + | took the third 6 samples at 2pm | ||
| + | |||
| + | took the fourth 6 samples at 10pm | ||
| + | |||
| + | '''on 11th, Sep''' | ||
| + | |||
| + | took the fifth 6 samples at 10am | ||
| + | |||
===pdark-GFP electro-transformation=== | ===pdark-GFP electro-transformation=== | ||
'''on 8th, Sep''' | '''on 8th, Sep''' | ||
Revision as of 08:44, 14 September 2015
Contents
Transformation
pdark natural transformation
in the evening of 11th, Sep
6th liquid segregation of pdark-HR
pcpcG2 electro-transformation
in the evening of 11th Sep
5th liquid segregation of pcpcG2-HR
first desalination test of pcpcG2-HR
on 7th, Sep
Inoculated 15 wildtype & 15 pcpcG2-HR (use pcpcG2-HR from colony 2) at 10pm
on 9th, Sep
Induced with green light at 10pm, added 22g/L NaCl & retinene, then took the first 6 samples (3 parallel samples for each)
on 10th, Sep
cultivated with red light at 10am, then took the second 6 samples,
took the third 6 samples at 2pm
took the fourth 6 samples at 10pm
on 11th, Sep
took the fifth 6 samples at 10am
pdark-GFP electro-transformation
on 8th, Sep
We picked 1 colony (only 1 colony appeared) from the pdark-GFP plate and spread them on another BG11 plate in line to do solid segregation