Difference between revisions of "Team:Danzi Kesh 8"

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<h1 style="color: #00043a"> Danzi Kesh 8 </h1>
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<title>Safie by Technion-Israel</title>
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<h2> Welcome to iGEM 2015! </h2>
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<meta http-equiv="content-type" content="text/html; charset=utf-8" />
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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<h4>Before you start: </h4>
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<p> Please read the following pages:</p>
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<ul>
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<!--[if lte IE 8]><link rel="stylesheet" href="css/ie/v8.css" /><![endif]-->
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<li> <a href="https://2015.igem.org/Requirements">Requirements page </a> </li>
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<link href="//maxcdn.bootstrapcdn.com/font-awesome/4.2.0/css/font-awesome.min.css" rel="stylesheet">
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<li> <a href="https://2015.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
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<h4> Styling your wiki </h4>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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<h4> Editing your wiki </h4>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<p> <a href="https://2015.igem.org/wiki/index.php?title=Team:Danzi_Kesh_8&action=edit"> Click here to edit this page! </a></p>
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<h4>Templates </h4>
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#contentSub, #search-controls, .firstHeading, #footer-box, #catlinks, #p-logo {
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<p> This year we have created templates for teams to use freely. More information on how to use and edit the templates can be found on the
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<a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation </a> page.</p>
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<h4>Tips</h4>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2015.igem.org/Calendar_of_Events">iGEM 2015 calendar</a> </li>
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<h4>Inspiration</h4>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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</ul>
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<h4> Uploading pictures and files </h4>
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<p> You can upload your pictures and files to the iGEM 2015 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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When you upload, set the "Destination Filename" to <code>Team:YourOfficialTeamName/NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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<a href="https://2015.igem.org/Special:Upload">CLICK HERE TO UPLOAD FILES</a>
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<body class="homepage">
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<div id="header">           
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<!-- Nav -->
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<nav id="nav">
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<ul>
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<li  id="parent"><a href="https://2014.igem.org/Team:Technion-Israel">Home</a></li>
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<li id="parent">
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<a href="https://2014.igem.org/Team:Technion-Israel/Project">Project</a>
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<ul class="sub1">
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<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Project#idea">The idea</a></li>
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<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Project#howitworks">How it works</a></li>
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<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Project#alpha">Alpha System</a></li>
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<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Project#beta">Beta System</a></li>
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<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Project#rna">RNA splint</a></li>
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<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Project#azo">Azobenzene</a></li>
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<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Project#hk">Histidine Kinase</a></li>
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<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Project#newmethod">New Standard</a></li>
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<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Project#protocol">Protocols</a></li>
 +
<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Project#notebook">Lab Notebook</a></li>
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<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Project#safety">Safety</a></li>
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</ul>
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</li>
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<li id="parent"><a href="https://2014.igem.org/Team:Technion-Israel/Modeling">Modeling</a>
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  <ul class="sub1">
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    <li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Modeling#whyworks">Why it should work</a></li>
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    <li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Modeling#whyfail">Why it should fail</a></li>
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    <li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Modeling#splint">RNA Splint</a></li>
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    <li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Modeling#biofilm">Synthetic Biofilm<br>Formation</a></li>
 +
  </ul>
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</li>
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 +
<li id="parent"><a href="https://2014.igem.org/Team:Technion-Israel/Experiments">Experiments</a>
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  <ul class="sub1">
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    <li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Experiments#gate1">Gate 1</a></li>
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    <li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Experiments#gate2">Gate 2</a></li>
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    <li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Experiments#rna">RNA Splint</a></li>
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    <li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Experiments#pompc">Pompc-RFP</a></li>
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    <li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Experiments#taz">TaZ</a></li>
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    <li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Experiments#mcherry">mCherry</a></li>
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    <li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Experiments#amilcp">amilCP</a></li>
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    <li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Experiments#azo">Azobenzene</a></li>
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  </ul>
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</li>
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<li id="parent">
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<a href="https://2014.igem.org/Team:Technion-Israel/HP">Policy &amp; Practices</a>
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<ul class="sub1">
 +
<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/HP#3lang">SynBio in 3 Languages</a></li>
 +
<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/HP#highschool">iGEM High Schools</a></li>
 +
<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/HP#lectures">SynBio Lectures</a></li>
 +
<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/HP#collab">Collaborations</a></li>
 +
<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/HP#art">Art</a></li>
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</ul>                             
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                                </li>
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<li id="parent">
 +
                                <a href="https://2014.igem.org/Team:Technion-Israel/Team">The Team</a>
 +
                                <ul class="sub1">
 +
<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Team#members">Members</a></li>
 +
<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Team#mentors">Mentors</a></li>
 +
<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Team#gallery">Gallery</a></li>
 +
<li id="child1"><a href="https://igem.org/Team.cgi?id=1343" target="_blank">Official Team<br>Profile</a></li>
 +
<li id="child1"><a href="https://www.facebook.com/iGEM2014.Technion" target="_blank">Official Facebook<br>Page</a></li>
 +
                                    </ul>
 +
                                </li>
 +
<li id="parent">
 +
                                <a href="https://2014.igem.org/Team:Technion-Israel/Support">Special Thanks</a>
 +
                                    <ul class="sub1">
 +
<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Support#headstart">Headstart Supporters</a></li>
 +
<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Support#attributions">Attributions</a></li>
 +
<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Support#sponsors">Sponsors</a></li>
 +
                                    </ul>
 +
                                </li>
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                                <li id="parent">
 +
                                <a href="https://2014.igem.org/Team:Technion-Israel/Judging">Judging</a>
 +
                                    <ul class="sub1">
 +
<li id="child1"><a href="https://igem.org/2014_Judging_Form?id=1343" target="_blank">Judging Form</a></li>
 +
<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Judging#biobrick">BioBricks</a></li>
 +
<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Judging#results">Results</a></li>
 +
                                    </ul>
 +
                                </li>
 +
<li style="vertical-align: bottom; height=50px;"><a href="https://2014.igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/2014/8/8b/Technion-Israel-igem.png"></a></li>
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</ul>
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                        </nav>
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</div>
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</div>
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<!-- The Project -->
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<div id="intro-wrapper" class="wrapper style1">
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<div class="title">The Project</div>
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<section id="intro" class="container">
 +
<img src="https://static.igem.org/mediawiki/2014/0/09/Technion-Israel-pic00.jpg" alt="" / class="image featured">
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<p class="style2"><strong>We are developing a low concentration bio-detector.</strong><br></p>
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                    <p class="style3">The system is based on an intricate <strong>gate construct</strong>,
 +
                    utilizes <strong>quorum-sensing</strong> and an altered <strong>two-component signalling system</strong>.
 +
                    In addition, groundbreaking organic synthesis of a photo-switching molecule – <strong>Azobenzene</strong> –
 +
is used in a pioneering attempt to mimic biofilm formation.<br>
 +
                    We have planned and built a low concentration detector using these elements.</p>
 +
<p><br><br></p>
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 +
<div class="feature-list">
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<div class="row">
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<div class="6u">
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<section>
 +
<h3 class="icon fa-lightbulb-o">The Idea</h3>
 +
<p>Read about our vision for our project. Find out more about the idea and where some R&amp;D can take us</p>
 +
                                    <ul class="actions actions-centered">
 +
<li><a href="https://2014.igem.org/Team:Technion-Israel/Project#idea" class="button style1 big">Read more</a></li>
 +
</ul>
 +
</section>
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</div>
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                            <div class="6u">
 +
<section>
 +
<h3 class="icon fa-exclamation">How it Works</h3>
 +
<p>Find out how we planed to achieve our goal for low concentration detection of different materials</p>
 +
                                    <ul class="actions actions-centered">
 +
<li><a href="https://2014.igem.org/Team:Technion-Israel/Project#howitworks" class="button style1 big">Read more</a></li>
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</ul>
 +
</section>
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</div>
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</div>
 +
<div class="row">
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<div class="6u">
 +
<section>
 +
<h3 class="icon fa-gear">Alpha System</h3>
 +
<p>Explore our first genetic logic gates design in detail, what happens in the system and how it can detect low concentrations</p>
 +
<ul class="actions actions-centered">
 +
<li><a href="https://2014.igem.org/Team:Technion-Israel/Project#alpha" class="button style1 big">Read more</a></li>
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</ul>
 +
</section>
 +
</div>
 +
<div class="6u">
 +
<section>
 +
<h3 class="icon fa-cogs">Beta System</h3>
 +
<p>Read about our second design of logic gates meant to reduce noise and help with more accurate detection</p>
 +
<ul class="actions actions-centered">
 +
<li><a href="https://2014.igem.org/Team:Technion-Israel/Project#beta" class="button style1 big">Read more</a></li>
 +
</ul>
 +
</section>
 +
</div>
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</div>
 +
                       
 +
<div class="row">
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<div class="6u">
 +
<section>
 +
<h3 class="icon fa-expand">RNA Splint</h3>
 +
<p>Find out about our plan to split any desired gene sequence to produce it under 2 different promoters and have the full protein self-assemble</p>
 +
<ul class="actions actions-centered">
 +
<li><a href="https://2014.igem.org/Team:Technion-Israel/Project#rna" class="button style1 big">Read more</a></li>
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</ul>
 +
</section>
 +
</div>
 +
                        <div class="6u">
 +
<section>
 +
<h3 class="icon fa-flask">Azobenzene</h3>
 +
<p>Read about our pioneering method for bacterial aggregation using organically synthesized Azobenzene molecules</p>
 +
<ul class="actions actions-centered">
 +
<li><a href="https://2014.igem.org/Team:Technion-Israel/Project#azo" class="button style1 big">Read more</a></li>
 +
</ul>
 +
</section>
 +
</div>
 +
</div>                       
 +
                       
 +
<div class="row">
 +
<div class="6u">
 +
<section>
 +
<h3 class="icon fa-tencent-weibo">Histidine Kinase</h3>
 +
<p>Read how the Histidine Kinase system in E.coli can be used for different applications and substances</p>
 +
<ul class="actions actions-centered">
 +
<li><a href="https://2014.igem.org/Team:Technion-Israel/Project#hk" class="button style1 big">Read more</a></li>
 +
</ul>
 +
                                </section>
 +
</div>
 +
<div class="6u">
 +
<section>
 +
<h3 class="icon fa-barcode">New Standard</h3>
 +
<p>Explore a new standard for faster DNA assembly based on<br> Gibson assembly</p>
 +
<ul class="actions actions-centered">
 +
<li><a href="https://2014.igem.org/Team:Technion-Israel/Project#newmethod" class="button style1 big">Read more</a></li>
 +
</ul>
 +
</section>
 +
</div>
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</div>                       
 +
                           
 +
<div class="row">
 +
<div class="6u">
 +
<section>
 +
<h3 class="icon fa-laptop">Modeling</h3>
 +
<p>Check out our extensive modeling for low concentration detection and bacterial aggregation<br></p>
 +
<ul class="actions actions-centered">
 +
<li><a href="https://2014.igem.org/Team:Technion-Israel/Modeling" class="button style1 big">Read more</a></li>
 +
</ul>
 +
</section>
 +
</div>
 +
<div class="6u">
 +
<section>
 +
<h3 class="icon fa-bar-chart">Experiments</h3>
 +
<p>Find out about the experiments we preformed and see the results. Read more about future experiments we planned</p>
 +
<ul class="actions actions-centered">
 +
<li><a href="https://2014.igem.org/Team:Technion-Israel/Experiments" class="button style1 big">Read more</a></li>
 +
</ul>
 +
</section>
 +
</div>
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</div>
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</div>
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</section>
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<div class="title">Policy &amp; Practices</div>
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<h2>In our Policy &amp; Practices project we focus on 3 principles:</h2>
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<p>Encouraging independent thinking and creativity among teens and youth<br>
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Progressing Arab-Jewish relations through the medium of science<br>
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Representing Israel as ambassadors in the international iGEM competition</p>
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<div class="row">
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<section>
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<h3 class="icon fa-language">SynBio in 3 Languages</h3>
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<p><br>All of our Policy &amp; Practices activities are related to our main concept of SynBio in 3 languages. Each activity was designed to include all members of Israeli society.</p>
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                                        <ul class="actions actions-centered">
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<li><a href="https://2014.igem.org/Team:Technion-Israel/HP#3lang" class="button style1 big">Read more</a></li>
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<h3 class="icon fa-graduation-cap">iGEM High Schools</h3>
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<p>We have launched the first iGEM High School team in Israel! We designed detaild lesson plans and found facilities for the project. The first group will start working for the 2015 competition.<br></p>
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<li><a href="https://2014.igem.org/Team:Technion-Israel/HP#highschool" class="button style1 big">Read more</a></li>
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<section>
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<h3 class="icon fa-user">SynBio Lectures</h3>
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<p>We have created detailed lesson plans including slideshows for children of all ages about Synthetic Biology in space and in water.  We used these lesson plans for our activities and they are available for your free use.</p>
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    <ul class="actions actions-centered">
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<li><a href="https://2014.igem.org/Team:Technion-Israel/HP#lectures" class="button style1 big">Read more</a></li>
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                                    </section>
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<section>
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<h3 class="icon fa-users">Collaborations</h3>
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<p><br>In the iGEM spirit we collaborated with the Paris_Bettencourt team on a few projects including a weekly newsletter, and with BGU_Israel on an experiment.<br></p>
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    <ul class="actions actions-centered">
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<li><a href="https://2014.igem.org/Team:Technion-Israel/HP#collab" class="button style1 big">Read more</a></li>
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<div class="title">The Team</div>
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<div class="title">Special Thanks</div>
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<h2>We would like to thank the following sponsors:</h2>
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<a href="http://lokey.technion.ac.il/Lokey/index.asp" target="_blank" class="image featured"><img src="https://static.igem.org/mediawiki/2014/d/de/Technion-Israel-lokey.jpg" alt="" /></a>
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<h3><a href="http://lokey.technion.ac.il/Lokey/index.asp" target="_blank">The Lorry I. Lokey center</a></h3>
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<!--<p>Lokey Interdisciplinary Center for Life Sciences and Engineering</p>-->
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<a href="http://www.technion.ac.il/en/" target="_blank" class="image featured"><img src="https://static.igem.org/mediawiki/2014/6/6c/Technion-Israel-technion.jpg" alt="" /></a>
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<h3><a href="http://www.technion.ac.il/en/" target="_blank">Technion</a></h3>
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<!--<p>Technion - Israel Institute of Technology -  public research university in Haifa, Israel.</p>-->
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<a href="http://www.sigmaaldrich.com/israel.html" target="_blank" class="image featured"><img src="https://static.igem.org/mediawiki/2014/4/43/Technion-Israel-sigma.jpg" alt="" /></a>
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<h3><a href="http://www.sigmaaldrich.com/israel.html" target="_blank">Sigma-Aldrich, Israel</a></h3>
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<!--<p>Sigma-Aldrich is a leading life science and high technology company.</p>-->
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<h3><a href="http://www.labotal.co.il/" target="_blank">Labotal</a></h3>
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<a href="http://www.bioanalytics.co.il/" target="_blank" class="image featured"><img src="https://static.igem.org/mediawiki/2014/7/78/Technion-Israel-bioan.jpg" alt="" /></a>
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<h3><a href="http://www.bioanalytics.co.il/" target="_blank">BioAnalytics</a></h3>
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<a href="http://www.danyel.co.il/" target="_blank" class="image featured"><img src="https://static.igem.org/mediawiki/2014/f/f4/Technion-Israel-dani.jpg" alt="" /></a>
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<h3><a href="http://www.danyel.co.il/" target="_blank">DanyelBiotech</a></h3>
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Revision as of 01:33, 15 June 2015

Safie by Technion-Israel

The Project

We are developing a low concentration bio-detector.

The system is based on an intricate gate construct, utilizes quorum-sensing and an altered two-component signalling system. In addition, groundbreaking organic synthesis of a photo-switching molecule – Azobenzene – is used in a pioneering attempt to mimic biofilm formation.
We have planned and built a low concentration detector using these elements.



The Idea

Read about our vision for our project. Find out more about the idea and where some R&D can take us

How it Works

Find out how we planed to achieve our goal for low concentration detection of different materials

Alpha System

Explore our first genetic logic gates design in detail, what happens in the system and how it can detect low concentrations

Beta System

Read about our second design of logic gates meant to reduce noise and help with more accurate detection

RNA Splint

Find out about our plan to split any desired gene sequence to produce it under 2 different promoters and have the full protein self-assemble

Azobenzene

Read about our pioneering method for bacterial aggregation using organically synthesized Azobenzene molecules

Histidine Kinase

Read how the Histidine Kinase system in E.coli can be used for different applications and substances

New Standard

Explore a new standard for faster DNA assembly based on
Gibson assembly

Modeling

Check out our extensive modeling for low concentration detection and bacterial aggregation

Experiments

Find out about the experiments we preformed and see the results. Read more about future experiments we planned

Policy & Practices

In our Policy & Practices project we focus on 3 principles:

Encouraging independent thinking and creativity among teens and youth
Progressing Arab-Jewish relations through the medium of science
Representing Israel as ambassadors in the international iGEM competition

SynBio in 3 Languages


All of our Policy & Practices activities are related to our main concept of SynBio in 3 languages. Each activity was designed to include all members of Israeli society.

iGEM High Schools

We have launched the first iGEM High School team in Israel! We designed detaild lesson plans and found facilities for the project. The first group will start working for the 2015 competition.

SynBio Lectures

We have created detailed lesson plans including slideshows for children of all ages about Synthetic Biology in space and in water. We used these lesson plans for our activities and they are available for your free use.

Collaborations


In the iGEM spirit we collaborated with the Paris_Bettencourt team on a few projects including a weekly newsletter, and with BGU_Israel on an experiment.

The Team
Special Thanks