Difference between revisions of "Team:Aalto-Helsinki/Parts"
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<p><b>Validation:</b> Our GFP brick has been fully sequenced, and the sequencing results were as expected. We have also been able to express the GFP after fusing it with an amphiphilic brick. This construct functioned under <a href="http://parts.igem.org/Part:BBa_K608003">BBa_K608003</a>, a strong constitutive promoter and a medium RBS. HS Slovenia Team also helped us validate this brick. They gained positive results of their construct with the GFP through colony PCR and analytical restrictions, but were unable to detect the fluerescence under UV light or functionality of the fused protein.</p> | <p><b>Validation:</b> Our GFP brick has been fully sequenced, and the sequencing results were as expected. We have also been able to express the GFP after fusing it with an amphiphilic brick. This construct functioned under <a href="http://parts.igem.org/Part:BBa_K608003">BBa_K608003</a>, a strong constitutive promoter and a medium RBS. HS Slovenia Team also helped us validate this brick. They gained positive results of their construct with the GFP through colony PCR and analytical restrictions, but were unable to detect the fluerescence under UV light or functionality of the fused protein.</p> | ||
<p style="color:red">The colony which produced the product seen in figure 3, well 10 was sent to the registry under the name <b><a href="http://parts.igem.org/Part:BBa_K1655001">BBa_K1655001</a></b>.</p> | <p style="color:red">The colony which produced the product seen in figure 3, well 10 was sent to the registry under the name <b><a href="http://parts.igem.org/Part:BBa_K1655001">BBa_K1655001</a></b>.</p> | ||
− | <p>Click <a href="">here</a> to download the full sequence of our Fusable GFP in pSB1C3 backbone.</p> | + | <p>Click <a href="https://static.igem.org/mediawiki/2015/5/51/Aalto-Helsinki_gfp_sequence_ah009.gb">here</a> to download the full sequence of our Fusable GFP in pSB1C3 backbone.</p> |
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<p><b>Validation:</b> Our amphiphilic brick's sequencing results were unclear, as the brick is mainly built of short repeats. <span style="color:red">Microscope pictures!</span></p> | <p><b>Validation:</b> Our amphiphilic brick's sequencing results were unclear, as the brick is mainly built of short repeats. <span style="color:red">Microscope pictures!</span></p> | ||
<p style="color:red">The colony which produced the product seen in figure 3, well 10 was sent to the registry under the name <b><a href="http://parts.igem.org/Part:BBa_K1655002">BBa_K1655002</a></b>.</p> | <p style="color:red">The colony which produced the product seen in figure 3, well 10 was sent to the registry under the name <b><a href="http://parts.igem.org/Part:BBa_K1655002">BBa_K1655002</a></b>.</p> | ||
− | <p>Click <a href="">here</a> to download the full sequence of the Amphiphilic Brick in pSB1C3 backbone.</p> | + | <p>Click <a href="https://static.igem.org/mediawiki/2015/4/41/Aalto-Helsinki_amph_sequence_ah009.gb">here</a> to download the full sequence of the Amphiphilic Brick in pSB1C3 backbone.</p> |
Revision as of 08:17, 15 September 2015