Difference between revisions of "Team:NRP-UEA-Norwich/Parts"
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<tr><td>Plac-GlgB-Rv3037c</td><td>pSB1C3</td><td>Branching enzyme + Acylation candidate 4 with IPTG inducible Promoter</td><td>Makes acetylated branched glycogen? (4)</td><td><a href="http://parts.igem.org/Part:BBa_K1618027">BBa_K1618027</a></td></tr> | <tr><td>Plac-GlgB-Rv3037c</td><td>pSB1C3</td><td>Branching enzyme + Acylation candidate 4 with IPTG inducible Promoter</td><td>Makes acetylated branched glycogen? (4)</td><td><a href="http://parts.igem.org/Part:BBa_K1618027">BBa_K1618027</a></td></tr> | ||
| − | <tr><td>Plac-Buk</td><td>pSB1C3</td><td>Butyrate kinase with IPTG inducible Promoter</td><td> | + | <tr><td>Plac-Buk</td><td>pSB1C3</td><td>Butyrate kinase with IPTG inducible Promoter</td><td>Reversible reaction that makes butyrate from butyryl phosphate</td><td><a href="http://parts.igem.org/Part:BBa_K1618012">BBa_K1618012</a></td></tr> |
| − | <tr><td>Plac-PTB</td><td>pSB1C3</td><td>Phosphotransbutrylase with IPTG inducible Promoter</td><td> | + | <tr><td>Plac-PTB</td><td>pSB1C3</td><td>Phosphotransbutrylase with IPTG inducible Promoter</td><td>Reversible reaction that makes butyryl-phosphate from butyryl-CoA</td><td><a href="http://parts.igem.org/Part:BBa_K1618013">BBa_K1618013</a></td></tr> |
| − | <tr><td>Plac-THL</td><td>pSB1C3</td><td>Thiolase with IPTG inducible Promoter</td><td>Makes | + | <tr><td>Plac-THL</td><td>pSB1C3</td><td>Thiolase with IPTG inducible Promoter</td><td>Makes Acetoacetyl-CoA from Acetyl-CoA</td><td><a href="http://parts.igem.org/Part:BBa_K1618014">BBa_K1618014</a></td></tr> |
| − | <tr><td>Plac-CRO</td><td>pSB1C3</td><td>Crotonase with IPTG inducible Promoter</td><td>Makes | + | <tr><td>Plac-CRO</td><td>pSB1C3</td><td>Crotonase with IPTG inducible Promoter</td><td>Makes Crotonyl-CoA from 3-hydroxybutyryl-CoA</td><td><a href="http://parts.igem.org/Part:BBa_K1618015">BBa_K1618015</a></td></tr> |
| − | <tr><td>Plac-BHBD</td><td>pSB1C3</td><td>β-hydroxybutyryl-CoA dehydrogenase with IPTG inducible Promoter</td><td>Makes | + | <tr><td>Plac-BHBD</td><td>pSB1C3</td><td>β-hydroxybutyryl-CoA dehydrogenase with IPTG inducible Promoter</td><td>Makes 3-hydroxybutyryl-CoA from Acetoacetyl-CoA </td><td><a href="http://parts.igem.org/Part:BBa_K1618016">BBa_K1618016</a></td></tr> |
| − | <tr><td>Plac-BCD</td><td>pSB1C3</td><td>Butyryl-CoA dehydrogenase with IPTG inducible Promoter</td><td>Makes | + | <tr><td>Plac-BCD</td><td>pSB1C3</td><td>Butyryl-CoA dehydrogenase with IPTG inducible Promoter</td><td>Makes Butyryl-CoA from Crotonyl-CoA</td><td><a href="http://parts.igem.org/Part:BBa_K1618017">BBa_K1618017</a></td></tr> |
<tr><td>Plac-EFTb</td><td>pSB1C3</td><td>Electron transfer protein β with IPTG inducible Promoter</td><td>Makes butyrate from acetate part 5</td><td><a href="http://parts.igem.org/Part:BBa_K1618018">BBa_K1618018</a></td></tr> | <tr><td>Plac-EFTb</td><td>pSB1C3</td><td>Electron transfer protein β with IPTG inducible Promoter</td><td>Makes butyrate from acetate part 5</td><td><a href="http://parts.igem.org/Part:BBa_K1618018">BBa_K1618018</a></td></tr> | ||
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<tr><td>Plac-EFTa</td><td>pSB1C3</td><td>Electron transfer protein α with IPTG inducible Promoter</td><td>Makes butyrate from acetate part 6</td><td>BBa_K1618019</td></tr> | <tr><td>Plac-EFTa</td><td>pSB1C3</td><td>Electron transfer protein α with IPTG inducible Promoter</td><td>Makes butyrate from acetate part 6</td><td>BBa_K1618019</td></tr> | ||
| − | <tr><td>Plac-Buk-PTB</td><td>pSB1C3</td><td>Butyrate to butyryl CoA pathway with IPTG inducible Promoter</td><td> | + | <tr><td>Plac-Buk-PTB</td><td>pSB1C3</td><td>Butyrate to butyryl CoA pathway with IPTG inducible Promoter</td><td> Reversible reaction that makes Butyrate from Butyryl-CoA</td><td><a href="http://parts.igem.org/Part:BBa_K1618020">BBa_K1618020</a></td></tr> |
| − | <tr><td>Plac-THL-CRO-BHBD-BCD-EFTb-EFTa</td><td>pSB1C3</td><td> | + | <tr><td>Plac-THL-CRO-BHBD-BCD-EFTb-EFTa</td><td>pSB1C3</td><td>Acetyl-CoA to Butyrate pathway with IPTG inducible Promoter </td><td>Makes butyrate from Acetyl-CoA full</td><td><a href="http://parts.igem.org/Part:BBa_K1618021">BBa_K1618021</a></td></tr> |
<tr><td>35S-MAO</td><td>?</td><td>Acylation candidate 1 with 35S Promoter</td><td>Acetylates starch? (1)</td><td><a href="http://parts.igem.org/Part:BBa_K1618033">BBa_K1618033</a></td></tr> | <tr><td>35S-MAO</td><td>?</td><td>Acylation candidate 1 with 35S Promoter</td><td>Acetylates starch? (1)</td><td><a href="http://parts.igem.org/Part:BBa_K1618033">BBa_K1618033</a></td></tr> | ||
Revision as of 10:16, 15 September 2015
Parts
| Part name | Backbone | Description | Function | Part number |
|---|---|---|---|---|
| Plac-GlgX | pSB1C3 | Debranching enzyme with IPTG inducible Promoter | Removes E. coli glycogen branches | BBa_K1618025 |
| GlgX | pSB1C3 | Debranching enzyme | BBa_K1618026 | |
| Plac-GlgB | pSB1C3 | Branching enzyme with IPTG inducible Promoter | Add E. coli glycogen branches | BBa_K1618022 |
| GlgB | pSB1C3 | Branching Enzyme | BBa_K1618000 | |
| Plac-MAO | pSB1C3 | Acylation candidate 1 with IPTG inducible Promoter | Acylates glycogen? (1) | BBa_K1618005 |
| MAO | pSB1C3 | Acylation Candidate 1 | BBa_K1618001 | |
| Plac-Rv3030 | pSB1C3 | Acylation candidate 2 with IPTG inducible Promoter | Acylates glycogen? (2) | BBa_K1618007 |
| Rv3030 | pSB1C3 | Acylation candidate 2 | BBa_K1618002 | |
| Plac-Rv3034c | pSB1C3 | Acylation candidate 3 with IPTG inducible Promoter | Acylates glycogen? (3) | BBa_K1618008 |
| Rv3034c | pSB1C3 | Acylation candidate 3 | BBa_K1618003 | |
| Plac-Rv3037c | pSB1C3 | Acylation candidate 4 with IPTG inducible Promoter | Acylates glycogen? (4) | BBa_K1618009 |
| Rv3037c | pSB1C3 | Acylation candidate 4 | BBa_K1618004 | |
| Plac-GlgB-Rv3030 | pSB1C3 | Branching enzyme + Acylation candidate 2 with IPTG inducible Promoter | Makes acetylated branched glycogen? (2) | BBa_K1618023 |
| Plac-GlgB-Rv3034c | pSB1C3 | Branching enzyme + Acylation candidate 3 with IPTG inducible Promoter | Makes acetylated branched glycogen? (3) | BBa_K1618024 |
| Plac-GlgB-Rv3037c | pSB1C3 | Branching enzyme + Acylation candidate 4 with IPTG inducible Promoter | Makes acetylated branched glycogen? (4) | BBa_K1618027 |
| Plac-Buk | pSB1C3 | Butyrate kinase with IPTG inducible Promoter | Reversible reaction that makes butyrate from butyryl phosphate | BBa_K1618012 |
| Plac-PTB | pSB1C3 | Phosphotransbutrylase with IPTG inducible Promoter | Reversible reaction that makes butyryl-phosphate from butyryl-CoA | BBa_K1618013 |
| Plac-THL | pSB1C3 | Thiolase with IPTG inducible Promoter | Makes Acetoacetyl-CoA from Acetyl-CoA | BBa_K1618014 |
| Plac-CRO | pSB1C3 | Crotonase with IPTG inducible Promoter | Makes Crotonyl-CoA from 3-hydroxybutyryl-CoA | BBa_K1618015 |
| Plac-BHBD | pSB1C3 | β-hydroxybutyryl-CoA dehydrogenase with IPTG inducible Promoter | Makes 3-hydroxybutyryl-CoA from Acetoacetyl-CoA | BBa_K1618016 |
| Plac-BCD | pSB1C3 | Butyryl-CoA dehydrogenase with IPTG inducible Promoter | Makes Butyryl-CoA from Crotonyl-CoA | BBa_K1618017 |
| Plac-EFTb | pSB1C3 | Electron transfer protein β with IPTG inducible Promoter | Makes butyrate from acetate part 5 | BBa_K1618018 |
| Plac-EFTa | pSB1C3 | Electron transfer protein α with IPTG inducible Promoter | Makes butyrate from acetate part 6 | BBa_K1618019 |
| Plac-Buk-PTB | pSB1C3 | Butyrate to butyryl CoA pathway with IPTG inducible Promoter | Reversible reaction that makes Butyrate from Butyryl-CoA | BBa_K1618020 |
| Plac-THL-CRO-BHBD-BCD-EFTb-EFTa | pSB1C3 | Acetyl-CoA to Butyrate pathway with IPTG inducible Promoter | Makes butyrate from Acetyl-CoA full | BBa_K1618021 |
| 35S-MAO | ? | Acylation candidate 1 with 35S Promoter | Acetylates starch? (1) | BBa_K1618033 |
| 35S-Rv3030 | ? | Acylation candidate 2 with 35S Promoter | Acetylates starch? (2) | BBa_K1618034 |
| 35S-Rv3034c | ? | Acylation candidate 3 with 35S Promoter | Acetylates starch? (3) | BBa_K1618035 |
| 35S-Rv3037c | ? | Acylation candidate 4 with 35S Promoter | Acetylates starch? (4) | BBa_K1618036 |
| 35S-GFP-MAO | ? | Acylation candidate 1 with 35S Promoter + GFP | Acetylates starch? (1) + GFP tag | BBa_K1618029 |
| 35S-GFP-Rv3030 | ? | Acylation candidate 2 with 35S Promoter + GFP | Acetylates starch? (2) + GFP tag | BBa_K1618030 |
| 35S-GFP-Rv3034c | ? | Acylation candidate 3 with 35S Promoter + GFP | Acetylates starch? (3) + GFP tag | BBa_K1618031 |
| 35S-GFP-Rv3037c | ? | Acylation candidate 4 with 35S Promoter + GFP | Acetylates starch? (4) + GFP tag | BBa_K1618032 |
| Chloroplast Transit Peptide | ? | Chloroplast Transit Peptide | BBa_K1618028 | |
| 35s Terminator | ? | Terminator | BBa_K1618037 |
