Toxin-mediated delivery toxins

In order to kill our bacteria which would imply the end of the game, we thought to use liposomes as toxin-mediated delivery. The idea is to create a bubble into the microfluidic chip containing the bacteria and the liposomes. As a result, when the bubble would touch the lasers, the liposomes would free up toxins that would kill the bacteria afterwards.

For this to be possible, we wanted to use photopolymerizable liposomes. The latter composed of photopolymerizable phospholipid (DC8,9PC) would release entrapped toxins upon visible light treatment (The photopolymerization occurs at 514 nm). Liposomes prepared from DC8,9PC alone form tube like structures that are not suitable for molecule entrapment, therefore, we thought to make a formulation containing a mixture of lipids (VIEW PROTOCOLE) .
In order to ensure a functional liposomes, we took as reference the article written by Yavlovich et al. (2011) entitled “A Novel class of Photo-triggerable liposomes containing DPPC:DC8,9PC as Vehicles for Delivery of Doxorubcin to Cells”.