Difference between revisions of "Team:KU Leuven/InterLabStudy/Results"

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Revision as of 14:38, 15 September 2015

Logo

Interlab Results

The results of our Interlab study are discussed in this section
The minipreped samples of the given devices were validated by restriction mapping using the enzymes NcoI and XhoI. These restriction enzymes left us with bands around 267, 364, 625 and 1724 basepairs. This was validated by a gel electrophoresis (figure 1). The numbers 101, 106 and 117 stand for the devices containing the promoters J23101, J23106 and J23117 respectively.


We made a Fluorescein standard graph and extrapolated the concentration of the GFP from the samples using the fluorescence and the absorbance values that were recorded. We went ahead with those values to calculate the mean and the standard deviation for our biological and technical replicates. We processed all the data in Microsoft excel.

Protein Kd (molecules) n Source
cI 20 2 2009 iGEM Aberdeen and Wang et al. (2009)
LuxR/AHL 3.7 1 2007 iGEM ETH Zurich and Basu et al. (2005)
PenI 17 2 Wittman et al. (1993)

Figure 1
Signaling cascade for motility in E.coli

Plasmid A Plasmid B

Figure 2
Left: plasmid A, Right: plasmid B. Click to enlarge

Results

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Contact

Address: Celestijnenlaan 200G room 00.08 - 3001 Heverlee
Telephone n°: +32(0)16 32 73 19
Mail: igem@chem.kuleuven.be