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| <h2 style="color:green;font-size:4em;">Notebook</h2> | | <h2 style="color:green;font-size:4em;">Notebook</h2> |
− | </article>
| + | |
| <div> | | <div> |
− | <p>We listed 23 pieces of Breaking News. Here are the links:</p> | + | <p class="p1" style="font-size:20px;">We listed 23 pieces of Breaking News. Here are the links: |
| <ul> | | <ul> |
− | <li>Successfully got the solution of CNC!! And it appeared Tyndall effect. | + | <a href="#event1 "><li> 15 members was picked out. ZJU-China 2015 team was set up~ Our story began. |
− | </li><li>Successfully checked in termites and tc protein family. | + | </li></a> <a href="#e1 " ><li> 1st Group meeting. Everyone was assigned with his/her own mission. Brainstorm began. |
− | </li><li>Got the genome of tcdA1 and tcdB1 in NCBI. Got TT01(a species of Photorhabdus luminescens) which could produce toxic protein. It would play an important role in our plan B. | + | </li></a> <a href="#e2 " ><li> Team registration completed and handed in team registration fee. |
− | </li><li>Did experiments(rainbow kit)related to synthetic biology in Zhejiang Science&Technology Museum with high school students and by this way promoted syn-bio. | + | </li></a> <a href="#e3 " ><li> 11th Group meeting. Decided our project: termite terminator. Allocated all members into 4 groups. Each group was responsible for a part of our project. |
− | </li><li>Got the E.coli strain ET12567 from Professor Li's Lab. Began the experiment about Streptomyces avermitilis. | + | </li></a> <a href="#e4 " ><li> Successfully got the solution of CNC!! And it appeared Tyndall effect. |
− | </li><li>iGEM Shanghai Tour. Took part in the conference held in NYU. Visited FDU and communicated with them. | + | </li></a> <a href="#e5 " ><li> Successfully checked in termites and tc protein family. |
− | </li><li>Got the solid CNC with lyophilization. Built the framework of the first web page. | + | </li></a> <a href="#e6 " ><li> Got the genome of tcdA1 and tcdB1 in NCBI. Got TT01(a species of Photorhabdus luminescens) which could produce toxic protein. It would play an important role in our plan B. |
− | </li><li>Our proposal had been accepted and our team would be awarded financial support from SYNENERGENE! Cheers~ | + | </li></a> <a href="#e7 " ><li> Did experiments(rainbow kit)related to synthetic biology in Zhejiang Science&Technology Museum with high school students and by this way promoted syn-bio. |
− | </li><li>Judge Haoqian Zhang from Peking University came to our lab. Frozen the spores of Streptomyces avermitilis. | + | </li></a> <a href="#e8 " ><li> Got the E.coli strain ET12567 from Professor Li's Lab. Began the experiment about Streptomyces avermitilis. |
− | </li><li>Went to shanghai for the interview of visa. 12 members successfully passed the interview. | + | </li></a> <a href="#e9 " ><li> iGEM Shanghai Tour. Took part in the conference held in NYU. Visited FDU and communicated with them. |
− | </li><li>Solved the diffusion equation. | + | </li></a> <a href="#e10 " ><li> Got the solid CNC with lyophilization. Built the framework of the first web page. |
− | </li><li>Attended NCTU meetup in Taiwan. Present our half-done project and communicated with other teams. Decided to help BNU and HUST. | + | </li></a> <a href="#e11 " ><li> Our proposal had been accepted and our team would be awarded financial support from SYNENERGENE! Cheers~ |
− | </li><li>Discussed helping BNU and HUST. Designed and alpha tested the Synbio-cards. Improved the modeling. | + | </li></a> <a href="#e12 " ><li> Judge Haoqian Zhang from Peking University came to our lab. Frozen the spores of Streptomyces avermitilis. |
− | </li><li>Giant Jamboree registration completed. | + | </li></a> <a href="#e13 " ><li> Went to shanghai for the interview of visa. 12 members successfully passed the interview. |
− | </li><li>34th Group meeting. Communicated with 2 members of SJTU in our lab. Began to write wiki. Attached more importance to safety. | + | </li></a> <a href="#e14 " ><li> Solved the diffusion equation. |
− | </li><li>Visited Shanghai Science&Technology Museum and promoted our Synbio-cards(Polypoly card). | + | </li></a> <a href="#e15 " ><li> Attended NCTU meetup in Taiwan. Present our half-done project and communicated with other teams. Decided to help BNU and HUST. |
− | </li><li>Completed our framework and philosophy of safety. | + | </li></a> <a href="#e16 " ><li> Discussed helping BNU and HUST. Designed and alpha tested the Synbio-cards. Improved the modeling. |
− | </li><li>Bacteria solution PCR: device-plu1537 and CDS-plu1537. The sequence of ermE+plasmid, Frr+plasmid and plu1537+plasmid were confirmed, which marked the success in standardization of 3 parts!! | + | </li></a> <a href="#e17 " ><li> Giant Jamboree registration completed. |
− | </li><li>Got our device for detecting and killing termites. | + | </li></a> <a href="#e18 " ><li> 34th Group meeting. Communicated with 2 members of SJTU in our lab. Began to write wiki. Attached more importance to safety. |
− | </li> | + | </li></a> <a href="#e19 " ><li> Visited Shanghai Science&Technology Museum and promoted our Synbio-cards(Polypoly card). |
| + | </li></a> <a href="#e20 " ><li> Completed our framework and philosophy of safety. |
| + | </li></a> <a href="#e21 " ><li> Bacteria solution PCR: device-plu1537 and CDS-plu1537. The sequence of ermE+plasmid, Frr+plasmid and plu1537+plasmid were confirmed, which marked the success in standardization of 3 parts!! |
| + | </li></a> <a href="#e22 " ><li> Got our device for detecting and killing termites. |
| + | </li></a> <a href="#e23 " ><li> |
| + | </li></a> </ul> |
| + | Croll down to see more. |
| + | </p> |
| | | |
− |
| |
− | </ul>
| |
| </div> | | </div> |
− | | + | </article> |
| <ul class="timeline"> | | <ul class="timeline"> |
| | | |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
− | <p>Seamless assembly: plasmid+OrfX, plasmid+metK. PCR: CDS-tcdA1. Double enzyme digestion: RFP+plasmid. Seamless assembly: CDS-plu0840. PCR and DNA gel extraction: CDS-tcdA1. Changed pSB1A3 to pSB1C3: plu0840, plu1537, tcdA1. Transformed them into E.coli DH5α, selected from single colonies and used single enzyme digestion to verify the got plasmids.</p> | + | <p id="e23">Seamless assembly: plasmid+OrfX, plasmid+metK. PCR: CDS-tcdA1. Double enzyme digestion: RFP+plasmid. Seamless assembly: CDS-plu0840. PCR and DNA gel extraction: CDS-tcdA1. Changed pSB1A3 to pSB1C3: plu0840, plu1537, tcdA1. Transformed them into E.coli DH5α, selected from single colonies and used single enzyme digestion to verify the got plasmids.</p> |
| </div> | | </div> |
| </div> | | </div> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
− | <p>The sequence of ermE+plasmid, Frr+plasmid and plu1537+plasmid were confirmed, which marked the success in standardization of 3 parts!!</p> | + | <p id="e22">The sequence of ermE+plasmid, Frr+plasmid and plu1537+plasmid were confirmed, which marked the success in standardization of 3 parts!!</p> |
| </div> | | </div> |
| </div> | | </div> |
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| <ul> | | <ul> |
| <li><p class="p1">PCR: tcdA1-M, tcdA1-R, tcdB1, plu0840L, plu0840R and plu1537.</p></li> | | <li><p class="p1">PCR: tcdA1-M, tcdA1-R, tcdB1, plu0840L, plu0840R and plu1537.</p></li> |
− | <li><p class="p1">Completed our framework and philosophy of safety.</p></li> | + | <li><p class="p1" id = "e21">Completed our framework and philosophy of safety.</p></li> |
| <li><p class="p1">Extracted the plasmid containing RFP from DH5α. PCR: RFP and confirmed by gel electrophoresis.</p></li> | | <li><p class="p1">Extracted the plasmid containing RFP from DH5α. PCR: RFP and confirmed by gel electrophoresis.</p></li> |
| </ul> | | </ul> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
− | <p>Visited Shanghai Science&Technology Museum and promoted our Synbio-cards(Polypoly card).</p> | + | <p id="e20">Visited Shanghai Science&Technology Museum and promoted our Synbio-cards(Polypoly card).</p> |
| </div> | | </div> |
| <img src="https://static.igem.org/mediawiki/2015/thumb/b/bb/8-8-3.jpg/800px-8-8-3.jpg" class="img-responsive"> | | <img src="https://static.igem.org/mediawiki/2015/thumb/b/bb/8-8-3.jpg/800px-8-8-3.jpg" class="img-responsive"> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
− | <p>34th Group meeting. Communicated with 2 members of SJTU in our lab. Began to write wiki. Attached more importance to safety.</p> | + | <p id="e19">34th Group meeting. Communicated with 2 members of SJTU in our lab. Began to write wiki. Attached more importance to safety.</p> |
| </div> | | </div> |
| </div> | | </div> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
− | <p>Giant Jamboree registration completed.</p> | + | <p id = "e18">Giant Jamboree registration completed.</p> |
| </div> | | </div> |
| <img src="https://static.igem.org/mediawiki/2015/thumb/e/e5/7-28.png/420px-7-28.png" class="img-responsive"> | | <img src="https://static.igem.org/mediawiki/2015/thumb/e/e5/7-28.png/420px-7-28.png" class="img-responsive"> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
− | <p>Discussed helping BNU and HUST. Designed and alpha tested the Synbio-cards. Improved the modeling.</p> | + | <p id="e17">Discussed helping BNU and HUST. Designed and alpha tested the Synbio-cards. Improved the modeling.</p> |
| </div> | | </div> |
| </div> | | </div> |
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| <p><small class="text-muted">7.19.2015-7.24.2015</small></p> | | <p><small class="text-muted">7.19.2015-7.24.2015</small></p> |
| </div> | | </div> |
− | <div class="tl-body"> | + | <div class="tl-body" id = "e16"> |
| <p>Attended NCTU meetup in Taiwan. Present our half-done project and communicated with other teams. Decided to help BNU and HUST. </p> | | <p>Attended NCTU meetup in Taiwan. Present our half-done project and communicated with other teams. Decided to help BNU and HUST. </p> |
| </div> | | </div> |
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| <p><small class="text-muted">6.14.2015</small></p> | | <p><small class="text-muted">6.14.2015</small></p> |
| </div> | | </div> |
− | <div class="tl-body">Solved the diffusion equation. | + | <div class="tl-body" id="e15">Solved the diffusion equation. |
| </div> | | </div> |
| <img src="https://static.igem.org/mediawiki/2015/3/3d/6-14-2.jpg" class="img-responsive"> | | <img src="https://static.igem.org/mediawiki/2015/3/3d/6-14-2.jpg" class="img-responsive"> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
− | <p>Went to shanghai for the interview of visa. 12 members successfully passed the interview. </p> | + | <p id="e14">Went to shanghai for the interview of visa. 12 members successfully passed the interview. </p> |
| </div> | | </div> |
| </div> | | </div> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
− | <p>Frozen the spores of Streptomyces avermitilis.</p> | + | <p id="e13">Frozen the spores of Streptomyces avermitilis.</p> |
| </div> | | </div> |
| <img src="https://static.igem.org/mediawiki/2015/thumb/0/03/6-6.jpeg/800px-6-6.jpeg" class="img-responsive"> | | <img src="https://static.igem.org/mediawiki/2015/thumb/0/03/6-6.jpeg/800px-6-6.jpeg" class="img-responsive"> |
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| <ul> | | <ul> |
| <li><p class="p1">Discussed the characterization of E.coli embedded in CNC, including TEM, SEM, confocal, FTIR and flow cytometry.</p></li> | | <li><p class="p1">Discussed the characterization of E.coli embedded in CNC, including TEM, SEM, confocal, FTIR and flow cytometry.</p></li> |
− | <li><p class="p1">Our proposal had been accepted and our team would be awarded financial support from SYNENERGENE! Cheers~</p></li> | + | <li><p class="p1" id ="e12">Our proposal had been accepted and our team would be awarded financial support from SYNENERGENE! Cheers~</p></li> |
| <li><p class="p1">Brainstormed how to do human practice and use the €5000. We came up with the Synbio-cards and Synbio-workshops.</p></li> | | <li><p class="p1">Brainstormed how to do human practice and use the €5000. We came up with the Synbio-cards and Synbio-workshops.</p></li> |
| </ul> | | </ul> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
− | <p>Got the solid CNC with lyophilization. Built the framework of the first web page.</p> | + | <p id ="e11">Got the solid CNC with lyophilization. Built the framework of the first web page.</p> |
| </div> | | </div> |
| <img src="https://static.igem.org/mediawiki/2015/thumb/0/0b/5-26.png/800px-5-26.png" class="img-responsive"> | | <img src="https://static.igem.org/mediawiki/2015/thumb/0/0b/5-26.png/800px-5-26.png" class="img-responsive"> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
− | <p>iGEM Shanghai Tour. Took part in the conference held in NYU. Visited FDU and communicated with them.</p> | + | <p id = e10>iGEM Shanghai Tour. Took part in the conference held in NYU. Visited FDU and communicated with them.</p> |
| </div> | | </div> |
| </div> | | </div> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
− | <p>Got the E.coli strain ET12567 from Professor Li's Lab. Began the experiment about Streptomyces avermitilis.</p> | + | <p id="e9">Got the E.coli strain ET12567 from Professor Li's Lab. Began the experiment about Streptomyces avermitilis.</p> |
| </div> | | </div> |
| </div> | | </div> |
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| <p><small class="text-muted">5.17.2015</small></p> | | <p><small class="text-muted">5.17.2015</small></p> |
| </div> | | </div> |
− | <div class="tl-body">Did experiments(rainbow kit)related to synthetic biology in Zhejiang Science&Technology Museum with high school students and by this way promoted syn-bio. | + | <div class="tl-body" id ="e8">Did experiments(rainbow kit)related to synthetic biology in Zhejiang Science&Technology Museum with high school students and by this way promoted syn-bio. |
| </div> | | </div> |
| <img src="https://static.igem.org/mediawiki/2015/thumb/a/a3/5-17.jpg/800px-5-17.jpg" class="img-responsive"> | | <img src="https://static.igem.org/mediawiki/2015/thumb/a/a3/5-17.jpg/800px-5-17.jpg" class="img-responsive"> |
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| <div class="tl-body"> | | <div class="tl-body"> |
| <ul> | | <ul> |
− | <li><p class="p1">Got the genome of tcdA1 and tcdB1 in NCBI.</p></li> | + | <li><p class="p1" id = "e7">Got the genome of tcdA1 and tcdB1 in NCBI.</p></li> |
| <li><p class="p1">Got TT01(a species of Photorhabdus luminescens) which could produce toxic protein. It would play an important role in our plan B.</p></li> | | <li><p class="p1">Got TT01(a species of Photorhabdus luminescens) which could produce toxic protein. It would play an important role in our plan B.</p></li> |
| </ul> | | </ul> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
− | <p>Successfully checked in termites and tc protein family.</p> | + | <p id = "e6">Successfully checked in termites and tc protein family.</p> |
| </div> | | </div> |
| <img src="https://static.igem.org/mediawiki/2015/0/02/5-12.png" class="img-responsive"> | | <img src="https://static.igem.org/mediawiki/2015/0/02/5-12.png" class="img-responsive"> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
− | <p class="p1">Successfully got the solution of CNC!! And it appeared Tyndall effect.</p> | + | <p class="p1" id = "e5">Successfully got the solution of CNC!! And it appeared Tyndall effect.</p> |
| </div> | | </div> |
| <img src="https://static.igem.org/mediawiki/2015/thumb/c/c1/5-6.jpg/800px-5-6.jpg" class="img-responsive"> | | <img src="https://static.igem.org/mediawiki/2015/thumb/c/c1/5-6.jpg/800px-5-6.jpg" class="img-responsive"> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
− | <p>11th Group meeting. Decided our project: termite terminator. Allocated all members into 4 groups. Each group was responsible for a part of our project. </p> | + | <p id = "e4">11th Group meeting. Decided our project: termite terminator. Allocated all members into 4 groups. Each group was responsible for a part of our project. </p> |
| </div> | | </div> |
| <img src="https://static.igem.org/mediawiki/2015/thumb/c/c0/4-19.jpg/800px-4-19.jpg" class="img-responsive"> | | <img src="https://static.igem.org/mediawiki/2015/thumb/c/c0/4-19.jpg/800px-4-19.jpg" class="img-responsive"> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
− | <p>Team application completed.</p> | + | <p id = e3>Team application completed.</p> |
| </div> | | </div> |
| <img src="https://static.igem.org/mediawiki/2015/thumb/0/04/3-25.png/431px-3-25.png" class="img-responsive"> | | <img src="https://static.igem.org/mediawiki/2015/thumb/0/04/3-25.png/431px-3-25.png" class="img-responsive"> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
− | <p>1st Group meeting. Everyone was assigned with his/her own mission. Brainstorm began.</p> | + | <p id="e2">1st Group meeting. Everyone was assigned with his/her own mission. Brainstorm began.</p> |
| </div> | | </div> |
| </div> | | </div> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
− | <p>15 members was picked out. ZJU-China 2015 team was set up~ Our story began.</p> | + | <p id ="event1">15 members was picked out. ZJU-China 2015 team was set up~ Our story began.</p> |
| </div> | | </div> |
| <img src="https://static.igem.org/mediawiki/2015/c/c8/1-30-2.jpg" class="img-responsive"> | | <img src="https://static.igem.org/mediawiki/2015/c/c8/1-30-2.jpg" class="img-responsive"> |