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Submitted Parts

Below mentioned are the submitted parts. To explore sequencing, characterization etc. click the links and you will be directed to the parts registry page.

Standard Two-hybrid System Devices

BBa_K1638005
Plac-T18: The T18 domain of CyaA with a lac promoter. Intented for use in the bacterial two-hybrid system, where a protein of interest can be fused to the C-terminal. The bacterial two-hybrid system allows detection of protein-protein interactions.

BBa_K1638003
Plac-T25: The T25 domain of CyaA with a lac promoter. Intented for use in the bacterial two-hybrid system, where a protein of interest can be fused to the C-terminal. The bacterial two-hybrid system allows detection of protein-protein interactions.

BBa_K1638032
PcstA-RFP-term-Plac-T18: The T18 domain of CyaA with a lac promoter. Intented for use in the bacterial two-hybrid system, where a protein of interest can be fused to the C-terminal. The bacterial two-hybrid system allows detection of protein-protein interactions. The cAMP-induced promoter PcstA induce transcription of RFP when a protein-protein interaction occurs and the T18 and T25 domains associate and catalyse the formation of cAMP.

BBa_K1638033
PcstA-RFP-term-Plac-T25: The T25 domain of CyaA with a lac promoter. Intented for use in the bacterial two-hybrid system, where a protein of interest can be fused to the C-terminal. The bacterial two-hybrid system allows detection of protein-protein interactions. The cAMP-induced promoter PcstA induce transcription of RFP when a protein-protein interaction occurs and the T18 and T25 domains associate and catalyse the formation of cAMP.

Control Devices

BBa_K1638011
Plac-T18-Zip: Leucine Zipper fused to the T18 domain of CyaA. The expression of the gene is under control of a lac promoter. This part can be used to validate the bacterial two-hybrid system as leucine zippers is known to form a homodimer.

BBa_K1638013
Plac-T25-Zip: Leucine Zipper fused to the T25 domain of CyaA. The expression of the gene is under control of a lac promoter. This part can be used to validate the bacterial two-hybrid system as leucine zippers is known to form a homodimer.

BBa_K1638031
PcstA-RFP-term-Plac-T18-Zip: Leucine Zipper fused to the T18 domain of CyaA. The expression of the gene is under control of a lac promoter. This part can be used to validate the bacterial two-hybrid system as leucine zippers is known to form a homodimer. The cAMP-induced promoter PcstA induce transcription of RFP when a positive interaction occurs and the T18 and T25 domains associate and catalyse the formation of cAMP.

BBa_K1638030
PcstA-RFP-term-Plac-T25-Zip: Leucine Zipper fused to the T25 domain of CyaA. The expression of the gene is under control of a lac promoter. This part can be used to validate the bacterial two-hybrid system as leucine zippers is known to form a homodimer. The cAMP-induced promoter PcstA induce transcription of RFP when a positive interaction occurs and the T18 and T25 domains associate and catalyse the formation of cAMP.

Screening Devices

BBa_K1638018
PLlac-T18-linker-hTrx_scaffold-(3xFlag): The human thioredoxin scaffold fused to the T18 domain of CyaA through a flexible linker. The human thioredoxin scaffold is a scaffold used for presentation of peptide aptamers. When a random nucleotide library is inserted into the scaffold, one can use the bacterial two-hybrid system to screen against specific targets. An affinity 3xFlag-tag is fused to the C-terminal for detection purposes.

BBa_K1638035
Plac-T18-linker-Intein-hTrx_scaffold-(3xFlag): The human thioredoxin scaffold fused to the T18 domain of CyaA through intein and a flexible linker. The human thioredoxin scaffold is a scaffold used for presentation of peptide aptamers. When a random nucleotide library is inserted into the scaffold, one can use the bacterial two-hybrid system to screen against specific targets. The intein is added for affinity purification purposes. This allows us to only purify the pure hTrx-based peptide aptamer. An affinity 3xFlag-tag is fused to the C-terminal for detection purposes.

BBa_K1638037
PcstA-RFP-term-Plac-T25-linker-CsrA: The carbon storage regulator CsrA fused to the T25 domain of the adenylate cyclase, CyaA. Made for the purpose to be a 'bait' in a peptide-aptamer screening using the hTrx-based peptide aptamer fused to the T18 domain of CyaA. The cAMP-induced promoter PcstA induce transcription of RFP when a positive interaction occurs and the T18 and T25 domains associate and catalyse the formation of cAMP.

BBa_K1638038
PcstA-RFP-term-Plac-T25-linker-YhbJ: The RNase adapter protein YhbJ fused to the T25 domain of the adenylate cyclase, CyaA. Made for the purpose to be a 'bait' in a peptide-aptamer screening using the hTrx-based peptide aptamer fused to the T18 domain of CyaA. The cAMP-induced promoter PcstA induce transcription of RFP when a positive interaction occurs and the T18 and T25 domains associate and catalyse the formation of cAMP.

BBa_K1638039
PcstA-RFP-term-Plac-T25-linker-Hfq: The RNA-binding protein Hfq fused to the T25 domain of the adenylate cyclase, CyaA. Made for the purpose to be a 'bait' in a peptide-aptamer screening using the hTrx-based peptide aptamer fused to the T18 domain of CyaA. The cAMP-induced promoter PcstA induce transcription of RFP when a positive interaction occurs and the T18 and T25 domains associate and catalyse the formation of cAMP.

Reporter Fusions

BBa_K1638009
Plac-T18-linker-GFP: Green Fluorescent Protein (GFP) fused to the T18 domain of the adenylate cyclase CyaA through a flexible linker. This part is used to check gene expression and validate that T18-fusion proteins will fold correctly.

BBa_K1638010
Plac-T25-linker-GFP: Green Fluorescent Protein (GFP) fused to the T25 domain of the adenylate cyclase CyaA through a flexible linker. This part is used to check gene expression and validate that T25-fusion proteins will fold correctly.

BBa_K1638007
linker-GFP: A flexible 10 amino acid linker fused to the N-terminal part of GFP. Intended for use in protein-GFP fusions, where this part can be suffixed to various proteins as a reporter of gene expression.

Others

BBa_K1638036
PLlac-Intein: This part consist of an intein from Saccharomyces cerevisiae VMA1 gene including a CBD-tag. This intein is a thiol-induced self-cleavable protein that enables the release of a C-terminal fused protein.

Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <groupparts> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.

Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.

Note

Note that parts must be documented on the Registry. This page serves to showcase the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.

Adding parts to the registry

You can add parts to the Registry at our Add a Part to the Registry link.

We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you do need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)

What information do I need to start putting my parts on the Registry?

The information needed to initially create a part on the Registry is:

Part Name
Part type
Creator
Sequence
Short Description (60 characters on what the DNA does)
Long Description (Longer description of what the DNA does)
Design considerations

We encourage you to put up much more information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page.

Inspiration

We have a created a collection of well documented parts that can help you get started.