Difference between revisions of "Team:SDU-Denmark/Tour53"
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<p><b>Below are our submitted parts.</b> If you want to dig deeper and explore sequencing, characterization etc. click the links and you will be directed to the parts registry page.</p> | <p><b>Below are our submitted parts.</b> If you want to dig deeper and explore sequencing, characterization etc. click the links and you will be directed to the parts registry page.</p> | ||
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Revision as of 20:27, 15 September 2015
Submitted Parts
Below are our submitted parts. If you want to dig deeper and explore sequencing, characterization etc. click the links and you will be directed to the parts registry page.
Standard Two-hybrid System Devices
BBa_K1638005
Plac-T18: The T18 domain of CyaA with a lac promoter. Intented for use in the bacterial two-hybrid system, where a protein of interest can be fused to the C-terminal. The bacterial two-hybrid system allows detection of protein-protein interactions.
BBa_K1638003
Plac-T25: The T25 domain of CyaA with a lac promoter. Intented for use in the bacterial two-hybrid system, where a protein of interest can be fused to the C-terminal. The bacterial two-hybrid system allows detection of protein-protein interactions.
BBa_K1638032
PcstA-RFP-term-Plac-T18: The T18 domain of CyaA with a lac promoter. Intented for use in the bacterial two-hybrid system, where a protein of interest can be fused to the C-terminal. The bacterial two-hybrid system allows detection of protein-protein interactions. The cAMP-induced promoter PcstA induce transcription of RFP when a protein-protein interaction occurs and the T18 and T25 domains associate and catalyse the formation of cAMP.
BBa_K1638033
PcstA-RFP-term-Plac-T25: The T25 domain of CyaA with a lac promoter. Intented for use in the bacterial two-hybrid system, where a protein of interest can be fused to the C-terminal. The bacterial two-hybrid system allows detection of protein-protein interactions. The cAMP-induced promoter PcstA induce transcription of RFP when a protein-protein interaction occurs and the T18 and T25 domains associate and catalyse the formation of cAMP.
Plac-T18: The T18 domain of CyaA with a lac promoter. Intented for use in the bacterial two-hybrid system, where a protein of interest can be fused to the C-terminal. The bacterial two-hybrid system allows detection of protein-protein interactions.
BBa_K1638003
Plac-T25: The T25 domain of CyaA with a lac promoter. Intented for use in the bacterial two-hybrid system, where a protein of interest can be fused to the C-terminal. The bacterial two-hybrid system allows detection of protein-protein interactions.
BBa_K1638032
PcstA-RFP-term-Plac-T18: The T18 domain of CyaA with a lac promoter. Intented for use in the bacterial two-hybrid system, where a protein of interest can be fused to the C-terminal. The bacterial two-hybrid system allows detection of protein-protein interactions. The cAMP-induced promoter PcstA induce transcription of RFP when a protein-protein interaction occurs and the T18 and T25 domains associate and catalyse the formation of cAMP.
BBa_K1638033
PcstA-RFP-term-Plac-T25: The T25 domain of CyaA with a lac promoter. Intented for use in the bacterial two-hybrid system, where a protein of interest can be fused to the C-terminal. The bacterial two-hybrid system allows detection of protein-protein interactions. The cAMP-induced promoter PcstA induce transcription of RFP when a protein-protein interaction occurs and the T18 and T25 domains associate and catalyse the formation of cAMP.
Control Devices
BBa_K1638011
Plac-T18-Zip: Leucine Zipper fused to the T18 domain of CyaA. The expression of the gene is under control of a lac promoter. This part can be used to validate the bacterial two-hybrid system as leucine zippers is known to form a homodimer.
BBa_K1638013
Plac-T25-Zip: Leucine Zipper fused to the T25 domain of CyaA. The expression of the gene is under control of a lac promoter. This part can be used to validate the bacterial two-hybrid system as leucine zippers is known to form a homodimer.
BBa_K1638031
PcstA-RFP-term-Plac-T18-Zip: Leucine Zipper fused to the T18 domain of CyaA. The expression of the gene is under control of a lac promoter. This part can be used to validate the bacterial two-hybrid system as leucine zippers is known to form a homodimer. The cAMP-induced promoter PcstA induce transcription of RFP when a positive interaction occurs and the T18 and T25 domains associate and catalyse the formation of cAMP.
BBa_K1638030
PcstA-RFP-term-Plac-T25-Zip: Leucine Zipper fused to the T25 domain of CyaA. The expression of the gene is under control of a lac promoter. This part can be used to validate the bacterial two-hybrid system as leucine zippers is known to form a homodimer. The cAMP-induced promoter PcstA induce transcription of RFP when a positive interaction occurs and the T18 and T25 domains associate and catalyse the formation of cAMP.
Plac-T18-Zip: Leucine Zipper fused to the T18 domain of CyaA. The expression of the gene is under control of a lac promoter. This part can be used to validate the bacterial two-hybrid system as leucine zippers is known to form a homodimer.
BBa_K1638013
Plac-T25-Zip: Leucine Zipper fused to the T25 domain of CyaA. The expression of the gene is under control of a lac promoter. This part can be used to validate the bacterial two-hybrid system as leucine zippers is known to form a homodimer.
BBa_K1638031
PcstA-RFP-term-Plac-T18-Zip: Leucine Zipper fused to the T18 domain of CyaA. The expression of the gene is under control of a lac promoter. This part can be used to validate the bacterial two-hybrid system as leucine zippers is known to form a homodimer. The cAMP-induced promoter PcstA induce transcription of RFP when a positive interaction occurs and the T18 and T25 domains associate and catalyse the formation of cAMP.
BBa_K1638030
PcstA-RFP-term-Plac-T25-Zip: Leucine Zipper fused to the T25 domain of CyaA. The expression of the gene is under control of a lac promoter. This part can be used to validate the bacterial two-hybrid system as leucine zippers is known to form a homodimer. The cAMP-induced promoter PcstA induce transcription of RFP when a positive interaction occurs and the T18 and T25 domains associate and catalyse the formation of cAMP.
Screening Devices
BBa_K1638018
PLlacO-1-T18-linker-hTrx_scaffold-(3xFlag): The human thioredoxin scaffold fused to the T18 domain of CyaA through a flexible linker. The human thioredoxin scaffold is a scaffold used for presentation of peptide aptamers. When a random nucleotide library is inserted into the scaffold, one can use the bacterial two-hybrid system to screen against specific targets. An affinity 3xFlag-tag is fused to the C-terminal for detection purposes. The part is under control of the hybrid PLlacO-1 promoter.
BBa_K1638035
Plac-T18-linker-Intein-hTrx_scaffold-(3xFlag): The human thioredoxin scaffold fused to the T18 domain of CyaA through intein and a flexible linker. The human thioredoxin scaffold is a scaffold used for presentation of peptide aptamers. When a random nucleotide library is inserted into the scaffold, one can use the bacterial two-hybrid system to screen against specific targets. The intein is added for affinity purification purposes. This allows us to only purify the pure hTrx-based peptide aptamer. An affinity 3xFlag-tag is fused to the C-terminal for detection purposes.
BBa_K1638037
PcstA-RFP-term-Plac-T25-linker-CsrA: The carbon storage regulator CsrA fused to the T25 domain of the adenylate cyclase, CyaA. Made for the purpose to be a 'bait' in a peptide-aptamer screening using the hTrx-based peptide aptamer fused to the T18 domain of CyaA. The cAMP-induced promoter PcstA induce transcription of RFP when a positive interaction occurs and the T18 and T25 domains associate and catalyse the formation of cAMP.
BBa_K1638038
PcstA-RFP-term-Plac-T25-linker-YhbJ: The RNase adapter protein YhbJ fused to the T25 domain of the adenylate cyclase, CyaA. Made for the purpose to be a 'bait' in a peptide-aptamer screening using the hTrx-based peptide aptamer fused to the T18 domain of CyaA. The cAMP-induced promoter PcstA induce transcription of RFP when a positive interaction occurs and the T18 and T25 domains associate and catalyse the formation of cAMP.
BBa_K1638039
PcstA-RFP-term-Plac-T25-linker-Hfq: The RNA-binding protein Hfq fused to the T25 domain of the adenylate cyclase, CyaA. Made for the purpose to be a 'bait' in a peptide-aptamer screening using the hTrx-based peptide aptamer fused to the T18 domain of CyaA. The cAMP-induced promoter PcstA induce transcription of RFP when a positive interaction occurs and the T18 and T25 domains associate and catalyse the formation of cAMP.
PLlacO-1-T18-linker-hTrx_scaffold-(3xFlag): The human thioredoxin scaffold fused to the T18 domain of CyaA through a flexible linker. The human thioredoxin scaffold is a scaffold used for presentation of peptide aptamers. When a random nucleotide library is inserted into the scaffold, one can use the bacterial two-hybrid system to screen against specific targets. An affinity 3xFlag-tag is fused to the C-terminal for detection purposes. The part is under control of the hybrid PLlacO-1 promoter.
BBa_K1638035
Plac-T18-linker-Intein-hTrx_scaffold-(3xFlag): The human thioredoxin scaffold fused to the T18 domain of CyaA through intein and a flexible linker. The human thioredoxin scaffold is a scaffold used for presentation of peptide aptamers. When a random nucleotide library is inserted into the scaffold, one can use the bacterial two-hybrid system to screen against specific targets. The intein is added for affinity purification purposes. This allows us to only purify the pure hTrx-based peptide aptamer. An affinity 3xFlag-tag is fused to the C-terminal for detection purposes.
BBa_K1638037
PcstA-RFP-term-Plac-T25-linker-CsrA: The carbon storage regulator CsrA fused to the T25 domain of the adenylate cyclase, CyaA. Made for the purpose to be a 'bait' in a peptide-aptamer screening using the hTrx-based peptide aptamer fused to the T18 domain of CyaA. The cAMP-induced promoter PcstA induce transcription of RFP when a positive interaction occurs and the T18 and T25 domains associate and catalyse the formation of cAMP.
BBa_K1638038
PcstA-RFP-term-Plac-T25-linker-YhbJ: The RNase adapter protein YhbJ fused to the T25 domain of the adenylate cyclase, CyaA. Made for the purpose to be a 'bait' in a peptide-aptamer screening using the hTrx-based peptide aptamer fused to the T18 domain of CyaA. The cAMP-induced promoter PcstA induce transcription of RFP when a positive interaction occurs and the T18 and T25 domains associate and catalyse the formation of cAMP.
BBa_K1638039
PcstA-RFP-term-Plac-T25-linker-Hfq: The RNA-binding protein Hfq fused to the T25 domain of the adenylate cyclase, CyaA. Made for the purpose to be a 'bait' in a peptide-aptamer screening using the hTrx-based peptide aptamer fused to the T18 domain of CyaA. The cAMP-induced promoter PcstA induce transcription of RFP when a positive interaction occurs and the T18 and T25 domains associate and catalyse the formation of cAMP.
Reporter Fusions
BBa_K1638009
Plac-T18-linker-GFP: Green Fluorescent Protein (GFP) fused to the T18 domain of the adenylate cyclase CyaA through a flexible linker. This part is used to check gene expression and validate that T18-fusion proteins will fold correctly.
BBa_K1638010
Plac-T25-linker-GFP: Green Fluorescent Protein (GFP) fused to the T25 domain of the adenylate cyclase CyaA through a flexible linker. This part is used to check gene expression and validate that T25-fusion proteins will fold correctly.
BBa_K1638007
linker-GFP: A flexible 10 amino acid linker fused to the N-terminal part of GFP. Intended for use in protein-GFP fusions, where this part can be suffixed to various proteins as a reporter of gene expression.
Plac-T18-linker-GFP: Green Fluorescent Protein (GFP) fused to the T18 domain of the adenylate cyclase CyaA through a flexible linker. This part is used to check gene expression and validate that T18-fusion proteins will fold correctly.
BBa_K1638010
Plac-T25-linker-GFP: Green Fluorescent Protein (GFP) fused to the T25 domain of the adenylate cyclase CyaA through a flexible linker. This part is used to check gene expression and validate that T25-fusion proteins will fold correctly.
BBa_K1638007
linker-GFP: A flexible 10 amino acid linker fused to the N-terminal part of GFP. Intended for use in protein-GFP fusions, where this part can be suffixed to various proteins as a reporter of gene expression.
Others
BBa_K1638036
PLlacO-1-Intein: This part consist of an intein from Saccharomyces cerevisiae VMA1 gene including a CBD-tag. This intein is a thiol-induced self-cleavable protein that enables the release of a C-terminal fused protein. The intein is under control of the hybrid PLlacO-1 promoter.
PLlacO-1-Intein: This part consist of an intein from Saccharomyces cerevisiae VMA1 gene including a CBD-tag. This intein is a thiol-induced self-cleavable protein that enables the release of a C-terminal fused protein. The intein is under control of the hybrid PLlacO-1 promoter.