Difference between revisions of "Team:Penn/Safety"

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<tr class="tableizer-firstrow"><th>Species name (including strain)</th><th>Risk Group</th><th>Risk Group Source</th><th>Disease risk to humans?</th><th>Part number/name</th><th>Natural function of part</th><th>How did you acquire it?</th></tr>
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<tr><td>E. coli NEB Turbo</td><td>1</td><td>DSMZ </td><td>Strain derived from K-12. May be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves.</td><td>pACBB-eGFP</td><td>pACBB backbone with multiple cloning site; GFP is a naturally occurring protein made by the jellyfish Aequorea victoria.</td><td>Obtained from the Chow lab at University of Pennsylvania</td></tr>
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<tr><td>E. coli NEB Turbo</td><td>1</td><td>DSMZ </td><td>Strain derived from K-12. May be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves.</td><td>pDAWN</td><td>Blue-light photoreceptor for light-activated gene expression</td><td>Obtained from the Chow lab at University of Pennsylvania</td></tr>
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<tr><td>E. coli NEB Turbo</td><td>1</td><td>DSMZ </td><td>Strain derived from K-12. May be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves.</td><td>BBa_K325909 pBAD + lux operon BioBrick on PSB1C3</td><td>Vibrio fisheri, a bacterium that symbiotically lives in the light organ of certain squid species. The species produces a small autoinducer molecule (AHL) with expression of the LuxI gene. When the population density of the bacteria is high, the AHL molecule binds a transcriptional regulator, LuxR. The regulator then binds to the promoter pLux and activates the lux box, causing luminescence. </td><td>Obtained from the BioBrick Registry</td></tr>
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<tr><td>E. coli NEB Turbo</td><td>1</td><td>DSMZ </td><td>Strain derived from K-12. May be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves.</td><td>pDawn + lacZ transformed into NEB turbo</td><td>Blue-light photoreceptor for light-activated gene expression</td><td>Obtained from the Chow lab at University of Pennsylvania</td></tr>
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<tr><td>E.Coli BL21</td><td>1</td><td>DSMZ </td><td>Strain derived from K-12. May be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves.</td><td>BBa_K325909 pBAD + lux operon BioBrick on PSB1C3</td><td>Vibrio fisheri, a bacterium that symbiotically lives in the light organ of certain squid species. The species produces a small autoinducer molecule (AHL) with expression of the LuxI gene. When the population density of the bacteria is high, the AHL molecule binds a transcriptional regulator, LuxR. The regulator then binds to the promoter pLux and activates the lux box, causing luminescence. </td><td>Obtained from the BioBrick Registry</td></tr>
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<tr><td>E.Coli BL21</td><td>1</td><td>DSMZ </td><td>Strain derived from K-12. May be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves.</td><td>pDawn + lacZ transformed into BL21</td><td>Blue-light photoreceptor for light-activated gene expression</td><td>Obtained from the Chow lab at University of Pennsylvania</td></tr>
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<tr><td>E.Coli BL21</td><td>1</td><td>DSMZ </td><td>Strain derived from K-12. May be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves.</td><td>pACBB + BBa_K325909 lux operon ligated by Penn iGEM 2015 for testing if ligation successful by inducing pBAD in biobrick with arabinose</td><td>Vibrio fisheri, a bacterium that symbiotically lives in the light organ of certain squid species. The species produces a small autoinducer molecule (AHL) with expression of the LuxI gene. When the population density of the bacteria is high, the AHL molecule binds a transcriptional regulator, LuxR. The regulator then binds to the promoter pLux and activates the lux box, causing luminescence. </td><td>Insert obtained from the BioBrick Registry; Backbone obtained from the Chow lab at the Universtiy of Pennsylvania</td></tr>
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<tr><td>E.Coli DH5-alpha</td><td>1</td><td>DSMZ </td><td>Strain derived from K-12. May be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves.</td><td>uninduced  pDawn RFP</td><td>Blue-light photoreceptor for light-activated gene expression</td><td>Obtained from the Chow lab at University of Pennsylvania</td></tr>
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<tr><td>E. coli NEB10</td><td>1</td><td>DSMZ </td><td>Strain derived from K-12. May be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves.</td><td>pACBB + pBAD + lux operon & pDawn + mRFP</td><td>Cotransformed plasmids</td><td>Plasmid 1 obtained from the biobrick registry and Plasmid 2 obtained from the Chow lab at the University of Pennsylvania</td></tr>
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<tr><td>SY102</td><td>1</td><td>DSMZ </td><td>Strain derived from K-12. May be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves.</td><td>luxAB+sulA and lux CDE+ CP38 </td><td>Modified lux box. Split-lux" system in which gene expression of lux AB is under control of the sulA inducible promoter, but lux CDE expression is under a constitutive promoter (CP25/CP38).  </td><td>Grown up and innoculated from agar spear sample received from Dr. Shimshon Belkin at the Hebrew University, Jerusalem, Israel</td></tr>
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<tr><td>SY104</td><td>1</td><td>DSMZ </td><td>Strain derived from K-12. May be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves.</td><td>luxAB+sulA and luxCDE + CP25</td><td>Modified lux box. Split-lux" system in which gene expression of lux AB is under control of the sulA inducible promoter, but lux CDE expression is under a constitutive promoter (CP25/CP38).  </td><td>Grown up and innoculated from agar spear sample received from Dr. Shimshon Belkin at the Hebrew University, Jerusalem, Israel</td></tr>
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<tr><td>HNS BW25113 Dhns::kan strain</td><td>1</td><td>DSMZ </td><td>Strain derived from K-12. May be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves.</td><td>pSB1C3 + pBAD + lux operon</td><td>Blue-light photoreceptor for light-activated gene expression</td><td>pDawn + mRFP transformed into the HNS mutant strain obtained from Dr. Goulian at the University of Pennsylvania</td></tr>
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<tr><td>HNS BW25113 Dhns::kan strain</td><td>1</td><td>DSMZ </td><td>Strain derived from K-12. May be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves.</td><td>pDawn + mRFP</td><td>Blue-light photoreceptor for light-activated gene expression</td><td>pDawn + mRFP transformed into the HNS mutant strain obtained from Dr. Goulian at the University of Pennsylvania</td></tr>
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Revision as of 19:59, 16 September 2015

SAFETY

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Species name (including strain)Risk GroupRisk Group SourceDisease risk to humans?Part number/nameNatural function of partHow did you acquire it?
E. coli NEB Turbo1DSMZ Strain derived from K-12. May be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves.pACBB-eGFPpACBB backbone with multiple cloning site; GFP is a naturally occurring protein made by the jellyfish Aequorea victoria.Obtained from the Chow lab at University of Pennsylvania
E. coli NEB Turbo1DSMZ Strain derived from K-12. May be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves.pDAWNBlue-light photoreceptor for light-activated gene expressionObtained from the Chow lab at University of Pennsylvania
E. coli NEB Turbo1DSMZ Strain derived from K-12. May be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves.BBa_K325909 pBAD + lux operon BioBrick on PSB1C3Vibrio fisheri, a bacterium that symbiotically lives in the light organ of certain squid species. The species produces a small autoinducer molecule (AHL) with expression of the LuxI gene. When the population density of the bacteria is high, the AHL molecule binds a transcriptional regulator, LuxR. The regulator then binds to the promoter pLux and activates the lux box, causing luminescence. Obtained from the BioBrick Registry
E. coli NEB Turbo1DSMZ Strain derived from K-12. May be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves.pDawn + lacZ transformed into NEB turboBlue-light photoreceptor for light-activated gene expressionObtained from the Chow lab at University of Pennsylvania
E.Coli BL211DSMZ Strain derived from K-12. May be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves.BBa_K325909 pBAD + lux operon BioBrick on PSB1C3Vibrio fisheri, a bacterium that symbiotically lives in the light organ of certain squid species. The species produces a small autoinducer molecule (AHL) with expression of the LuxI gene. When the population density of the bacteria is high, the AHL molecule binds a transcriptional regulator, LuxR. The regulator then binds to the promoter pLux and activates the lux box, causing luminescence. Obtained from the BioBrick Registry
E.Coli BL211DSMZ Strain derived from K-12. May be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves.pDawn + lacZ transformed into BL21Blue-light photoreceptor for light-activated gene expressionObtained from the Chow lab at University of Pennsylvania
E.Coli BL211DSMZ Strain derived from K-12. May be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves.pACBB + BBa_K325909 lux operon ligated by Penn iGEM 2015 for testing if ligation successful by inducing pBAD in biobrick with arabinoseVibrio fisheri, a bacterium that symbiotically lives in the light organ of certain squid species. The species produces a small autoinducer molecule (AHL) with expression of the LuxI gene. When the population density of the bacteria is high, the AHL molecule binds a transcriptional regulator, LuxR. The regulator then binds to the promoter pLux and activates the lux box, causing luminescence. Insert obtained from the BioBrick Registry; Backbone obtained from the Chow lab at the Universtiy of Pennsylvania
E.Coli DH5-alpha1DSMZ Strain derived from K-12. May be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves.uninduced pDawn RFPBlue-light photoreceptor for light-activated gene expressionObtained from the Chow lab at University of Pennsylvania
E. coli NEB101DSMZ Strain derived from K-12. May be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves.pACBB + pBAD + lux operon & pDawn + mRFPCotransformed plasmidsPlasmid 1 obtained from the biobrick registry and Plasmid 2 obtained from the Chow lab at the University of Pennsylvania
SY1021DSMZ Strain derived from K-12. May be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves.luxAB+sulA and lux CDE+ CP38 Modified lux box. Split-lux" system in which gene expression of lux AB is under control of the sulA inducible promoter, but lux CDE expression is under a constitutive promoter (CP25/CP38). Grown up and innoculated from agar spear sample received from Dr. Shimshon Belkin at the Hebrew University, Jerusalem, Israel
SY1041DSMZ Strain derived from K-12. May be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves.luxAB+sulA and luxCDE + CP25Modified lux box. Split-lux" system in which gene expression of lux AB is under control of the sulA inducible promoter, but lux CDE expression is under a constitutive promoter (CP25/CP38). Grown up and innoculated from agar spear sample received from Dr. Shimshon Belkin at the Hebrew University, Jerusalem, Israel
HNS BW25113 Dhns::kan strain1DSMZ Strain derived from K-12. May be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves.pSB1C3 + pBAD + lux operonBlue-light photoreceptor for light-activated gene expressionpDawn + mRFP transformed into the HNS mutant strain obtained from Dr. Goulian at the University of Pennsylvania
HNS BW25113 Dhns::kan strain1DSMZ Strain derived from K-12. May be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves.pDawn + mRFPBlue-light photoreceptor for light-activated gene expressionpDawn + mRFP transformed into the HNS mutant strain obtained from Dr. Goulian at the University of Pennsylvania