Difference between revisions of "Team:Aalto-Helsinki/Results"
(+mentioned violacein idea in amphiphilic) |
(sidenav working) |
||
Line 51: | Line 51: | ||
<ul id="sidenav" class="nav nav-stacked"><!-- nav-pills if we want rounded corners --> | <ul id="sidenav" class="nav nav-stacked"><!-- nav-pills if we want rounded corners --> | ||
− | <li><a href="# | + | <li><a href="#" data-scroll="overview"><h3>Overview</h3></a></li> |
− | <li><a href="# | + | <li><a href="#" data-scroll="propanepathway"><h3>Propane pathway</h3></a></li> |
− | <li><a href="# | + | <li><a href="#" data-scroll="continuous"><h3>Continuous production</h3></a></li> |
− | <li><a href="# | + | <li><a href="#" data-scroll="amphiphilic"><h3>Amphiphilic protein</h3></a></li> |
− | <li><a href="# | + | <li><a href="#" data-scroll="gfp"><h3>N-terminally fusable GFP</h3></a></li> |
− | <li><a href="# | + | <li><a href="#" data-scroll="hp"><h3>Combining modeling and experimental work in iGEM</h3></a></li> |
− | <li><a href="# | + | <li><a href="#" data-scroll="software"><h3>The HumHub report and Collab Seeker</h3></a></li> |
<li><a href="#"><h3 style="border-top:solid;">To the top</h3></a></li> | <li><a href="#"><h3 style="border-top:solid;">To the top</h3></a></li> | ||
</ul> | </ul> | ||
Line 66: | Line 66: | ||
<h1>Results</h1> | <h1>Results</h1> | ||
+ | |||
+ | |||
<!-- Overview below --> | <!-- Overview below --> | ||
+ | <section id="overview" class="active" data-anchor="overview"> | ||
− | <h2 | + | <h2 >Overview</h2> |
needs to be written | needs to be written | ||
+ | |||
+ | </p> | ||
<!-- Overview above --> | <!-- Overview above --> | ||
− | |||
+ | |||
+ | <!-- Propane pathway below --> | ||
+ | <section id="propanepathway" data-anchor="propanepathway"> | ||
<h2 id="propane pathway">Propane pathway</h2> | <h2 id="propane pathway">Propane pathway</h2> | ||
Line 80: | Line 87: | ||
<p>Microbially produced propane holds enormous promise as a potential replacement of portable fossil fuels, but the propane yields with current biological pathways are low. The pathway is complex, and to help concentrate engineering efforts on its critical parts, better quantitative understanding of the pathway is required. Our goals were to build a mathematical model of the pathway to better understand it and create biobricks of the propane pathway to help future teams and researchers to continue improving it.</p> | <p>Microbially produced propane holds enormous promise as a potential replacement of portable fossil fuels, but the propane yields with current biological pathways are low. The pathway is complex, and to help concentrate engineering efforts on its critical parts, better quantitative understanding of the pathway is required. Our goals were to build a mathematical model of the pathway to better understand it and create biobricks of the propane pathway to help future teams and researchers to continue improving it.</p> | ||
− | <figure style="float:right;margin-left:20px;"> | + | |
+ | <h3>Outcome</h3> | ||
+ | |||
+ | <figure style="float:right;margin-left:20px;margin-bottom:3%;"> | ||
<img src="https://static.igem.org/mediawiki/2015/a/aa/Aalto-Helsinki_bottleneck_both.png" style="width:400px;"/> | <img src="https://static.igem.org/mediawiki/2015/a/aa/Aalto-Helsinki_bottleneck_both.png" style="width:400px;"/> | ||
<figcaption><b>Figure 1:</b> Illustrative figure of the bottleneck results of our pathway.</figcaption> | <figcaption><b>Figure 1:</b> Illustrative figure of the bottleneck results of our pathway.</figcaption> | ||
</figure> | </figure> | ||
− | |||
<ul style="list-style-type:disc"> | <ul style="list-style-type:disc"> | ||
<li><p>Built <a href="https://2015.igem.org/Team:Aalto-Helsinki/Modeling_propane">a model of the pathway</a> based on known kinetic properties of the enzymes</p></li> | <li><p>Built <a href="https://2015.igem.org/Team:Aalto-Helsinki/Modeling_propane">a model of the pathway</a> based on known kinetic properties of the enzymes</p></li> | ||
Line 95: | Line 104: | ||
</ul> | </ul> | ||
+ | |||
+ | </section> | ||
<!-- Propane pathway above --> | <!-- Propane pathway above --> | ||
+ | |||
+ | |||
<!-- Continuous production below --> | <!-- Continuous production below --> | ||
+ | <section id="continuous" data-anchor="continuous"> | ||
− | <h2 | + | <h2 >Continuous production</h2> |
<figure style="float:right;margin-left:20px;"> | <figure style="float:right;margin-left:20px;"> | ||
Line 117: | Line 131: | ||
</ul> | </ul> | ||
+ | </section> | ||
<!-- Continuous production above --> | <!-- Continuous production above --> | ||
<!-- Amphiphilic protein below --> | <!-- Amphiphilic protein below --> | ||
+ | <section id="amphiphilic" data-anchor="amphiphilic"> | ||
− | <h2 | + | <h2 >Amphiphilic protein</h2> |
<h3>Background</h3> | <h3>Background</h3> | ||
<p>Amphiphilic proteins are synthetic proteins consisting of a hydrophilic and a hydrophobic domain that have been shown to spontaneously form micellar and vesicular structures. We were interested whether these structures could be used as scaffolds to have subsequent enzymes of the propane pathway in close proximity. To study if fusing enzymes to the proteins would disrupt micelle formation and whether our idea could enhance propane output we wanted to build models.</p> | <p>Amphiphilic proteins are synthetic proteins consisting of a hydrophilic and a hydrophobic domain that have been shown to spontaneously form micellar and vesicular structures. We were interested whether these structures could be used as scaffolds to have subsequent enzymes of the propane pathway in close proximity. To study if fusing enzymes to the proteins would disrupt micelle formation and whether our idea could enhance propane output we wanted to build models.</p> | ||
− | <figure style="float:right;margin-left:20px;"> | + | |
+ | <h3>Outcome</h3> | ||
+ | |||
+ | <figure style="float:right;margin-left:20px;margin-bottom:3%;"> | ||
<img src="https://static.igem.org/mediawiki/2015/e/e4/Aalto-Helsinki_micelle_circle_approach_2.png" style="width:300px;"/> | <img src="https://static.igem.org/mediawiki/2015/e/e4/Aalto-Helsinki_micelle_circle_approach_2.png" style="width:300px;"/> | ||
<figcaption><b>Figure 2:</b> 2d simplification of the micelle structure</figcaption> | <figcaption><b>Figure 2:</b> 2d simplification of the micelle structure</figcaption> | ||
</figure> | </figure> | ||
− | |||
<ul style="list-style-type:disc"> | <ul style="list-style-type:disc"> | ||
<li><p><a href="https://2015.igem.org/Team:Aalto-Helsinki/Modeling_synergy">Built a stochastic synergy model</a> in Python, simulating enzyme function in cases where two subsequent enzymes stay in close proximity to each other as opposed to moving around freely in a cell</p></li> | <li><p><a href="https://2015.igem.org/Team:Aalto-Helsinki/Modeling_synergy">Built a stochastic synergy model</a> in Python, simulating enzyme function in cases where two subsequent enzymes stay in close proximity to each other as opposed to moving around freely in a cell</p></li> | ||
Line 141: | Line 159: | ||
</ul> | </ul> | ||
+ | </section> | ||
<!-- Amphiphilic protein above --> | <!-- Amphiphilic protein above --> | ||
+ | |||
<!-- N-terminally fusable GFP below --> | <!-- N-terminally fusable GFP below --> | ||
+ | <section id="gfp" data-anchor="gfp"> | ||
− | <h2 | + | <h2>N-terminally fusable GFP</h2> |
<h3>Background</h3> | <h3>Background</h3> | ||
Line 158: | Line 179: | ||
GFP microscope pic | GFP microscope pic | ||
+ | </section> | ||
<!-- N-terminally fusable GFP above --> | <!-- N-terminally fusable GFP above --> | ||
<!-- Combining modeling and experimental work in iGEM below --> | <!-- Combining modeling and experimental work in iGEM below --> | ||
+ | <section id="hp" data-anchor="hp"> | ||
− | <h2 | + | <h2>Combining modeling and experimental work in iGEM</h2> |
<h3>Background</h3> | <h3>Background</h3> | ||
<p>Mathematical modeling is a key component of synthetic biology and also played a central role in our project. Collaboration between modelers and biologists can however be challenging, something we also noticed in our project. We wanted to study how iGEM teams tackle these challenges and are able to integrate modeling to their experimentation.</p> | <p>Mathematical modeling is a key component of synthetic biology and also played a central role in our project. Collaboration between modelers and biologists can however be challenging, something we also noticed in our project. We wanted to study how iGEM teams tackle these challenges and are able to integrate modeling to their experimentation.</p> | ||
− | <figure style="float:right;margin-left:20px;"> | + | <figure style="float:right;margin-left:20px;margin-top:2%;margin-bottom:2%;"> |
<img src="https://static.igem.org/mediawiki/2015/9/9d/Aalto-Helsinki_study_fields.jpg" style="width:400px;"/> | <img src="https://static.igem.org/mediawiki/2015/9/9d/Aalto-Helsinki_study_fields.jpg" style="width:400px;"/> | ||
<figcaption><b>Figure 3:</b> Educational background of questionnaire respondents, 2014 iGEM </br>participants and professional synthetic biology researchers categorized.</br> "Mathematical" includes mathematics, computer science and physics.</figcaption> | <figcaption><b>Figure 3:</b> Educational background of questionnaire respondents, 2014 iGEM </br>participants and professional synthetic biology researchers categorized.</br> "Mathematical" includes mathematics, computer science and physics.</figcaption> | ||
Line 184: | Line 207: | ||
</ul> | </ul> | ||
+ | </section> | ||
<!-- Combining modeling and experimental work in iGEM above --> | <!-- Combining modeling and experimental work in iGEM above --> | ||
+ | |||
<div style="clear:both;"></div> | <div style="clear:both;"></div> | ||
+ | |||
<!-- HumHub report and Collab Seeker below --> | <!-- HumHub report and Collab Seeker below --> | ||
+ | <section id="software" data-anchor="software"> | ||
− | <h2 | + | <h2>HumHub report and Collab Seeker</h2> |
<h3>Background</h3> | <h3>Background</h3> | ||
Line 203: | Line 230: | ||
pic of collab seeker? | pic of collab seeker? | ||
+ | |||
+ | </section> | ||
<!-- HumHub report and Collab Seeker above --> | <!-- HumHub report and Collab Seeker above --> | ||
Line 242: | Line 271: | ||
<p>To read our thoughts on future prospects and on how to carry on from where we left, please see our <a href="https://2015.igem.org/Team:Aalto-Helsinki/Future">Future page</a>.</p> | <p>To read our thoughts on future prospects and on how to carry on from where we left, please see our <a href="https://2015.igem.org/Team:Aalto-Helsinki/Future">Future page</a>.</p> | ||
+ | |||
+ | |||
+ | |||
+ | <p style="margin-bottom:0;padding-bottom:10%;"></p> | ||
</div> | </div> |
Revision as of 05:16, 16 September 2015