Difference between revisions of "Template:SJTU-BioX-Shanghai/Notebook/Transformation/w10"
(→first desalination test of pcpcG2-HR) |
|||
| Line 12: | Line 12: | ||
===first desalination test of pcpcG2-HR=== | ===first desalination test of pcpcG2-HR=== | ||
| − | + | ||
| + | =====Sep. 7===== | ||
Inoculated 15 wildtype & 15 pcpcG2-HR (use pcpcG2-HR from colony 2) at 10pm. | Inoculated 15 wildtype & 15 pcpcG2-HR (use pcpcG2-HR from colony 2) at 10pm. | ||
| − | + | =====Sep. 9===== | |
Induced with green light at 10pm, added 22g/L NaCl & retinene, then took the first 6 samples (3 parallel samples for each). | Induced with green light at 10pm, added 22g/L NaCl & retinene, then took the first 6 samples (3 parallel samples for each). | ||
| − | + | =====Sep. 10===== | |
Cultivated with red light at 10am, then took the second 6 samples, | Cultivated with red light at 10am, then took the second 6 samples, | ||
| Line 28: | Line 29: | ||
Took the fourth 6 samples at 10pm. | Took the fourth 6 samples at 10pm. | ||
| − | + | =====Sep. 11===== | |
Took the fifth 6 samples at 10am. | Took the fifth 6 samples at 10am. | ||
Revision as of 08:54, 16 September 2015
Contents
Transformation
pdark natural transformation
in the evening of 11th, Sep
6th liquid segregation of pdark-HR
pcpcG2 electro-transformation
in the evening of 11th Sep
5th liquid segregation of pcpcG2-HR
first desalination test of pcpcG2-HR
Sep. 7
Inoculated 15 wildtype & 15 pcpcG2-HR (use pcpcG2-HR from colony 2) at 10pm.
Sep. 9
Induced with green light at 10pm, added 22g/L NaCl & retinene, then took the first 6 samples (3 parallel samples for each).
Sep. 10
Cultivated with red light at 10am, then took the second 6 samples,
Took the third 6 samples at 2pm.
Took the fourth 6 samples at 10pm.
Sep. 11
Took the fifth 6 samples at 10am.
pdark-GFP electro-transformation
on 8th, Sep
We picked 1 colony (only 1 colony appeared) from the pdark-GFP plate and spread them on another BG11 plate in line to do solid segregation