Difference between revisions of "Team:Toulouse/Design"

Line 81: Line 81:
 
genetically modified organisms in a field and more, associated with  
 
genetically modified organisms in a field and more, associated with  
 
edible products underlies both law enforcement and applying of
 
edible products underlies both law enforcement and applying of
  regulations and public perception account about it. In this context,  
+
  regulations and public perception account about it.  
we searched a device being able to isolate our engineered bacteria from  
+
In this context, we designed a device being able to isolate our engineered bacteria from  
 
  the environment, but allowing its growth and metabolism. We found the  
 
  the environment, but allowing its growth and metabolism. We found the  
  project of the team iGEM Groningen 2012 which used the polymer TPX® in  
+
  project of the iGEM Groningen 2012 team which used the polymer TPX® in  
 
  order to contain their bacteria separated of the meat [1]. Thus, we  
 
  order to contain their bacteria separated of the meat [1]. Thus, we  
 
  decided to ask them TPX® characteristics and their supplier. We obtained
 
  decided to ask them TPX® characteristics and their supplier. We obtained
 
  some samples of TPX®, enough to perform tests.  
 
  some samples of TPX®, enough to perform tests.  
 +
To check if our device can be safe and feasible, several tests have been performed:
 
<br><br>
 
<br><br>
 
The impermeability of bacteria through the bag of TPX® has been tested.   
 
The impermeability of bacteria through the bag of TPX® has been tested.   

Revision as of 10:03, 16 September 2015

iGEM Toulouse 2015

Device


Trap Construction

TPX Bag

Safety tests

TrApiColi has been designed in order to take into account ethical reflection, safety and ease of use for beekeepers. The use of genetically modified organisms in a field and more, associated with edible products underlies both law enforcement and applying of regulations and public perception account about it. In this context, we designed a device being able to isolate our engineered bacteria from the environment, but allowing its growth and metabolism. We found the project of the iGEM Groningen 2012 team which used the polymer TPX® in order to contain their bacteria separated of the meat [1]. Thus, we decided to ask them TPX® characteristics and their supplier. We obtained some samples of TPX®, enough to perform tests. To check if our device can be safe and feasible, several tests have been performed:

The impermeability of bacteria through the bag of TPX® has been tested.

Growth culture tests in TPX®

It has been performed in order to test if the strain E. coli BW25113 can grow in a TPX® bag (ie. in microaerobic conditions, without agitation and miming batch culture condition), as it would be in the field.

References

  • [1] REFERENCE 1 Vers la page Groningen 2012
  • [2] REFERENCE 2 AVEC UN LIEN See more
  • [3] REFERENCE 2 AVEC UN LIEN qui ouvre dans une nouvelle fenêtre See more