Difference between revisions of "Team:KU Leuven/Research/Results"
| Line 84: | Line 84: | ||
<div class="part"> | <div class="part"> | ||
| − | <b>Double Knockouts</b> | + | |
| − | < | + | <p><b>Double Knockouts</b><br/> |
| + | The Centre of Microbial and Plant Genetics (KU Leuven) provided us with three E.coli K-12 strains, with each one representing the knockout for the genes tar, tsr or cheZ. The kanamycin cassette of the tar knock-out strain was removed by the enzyme flippase on pCP20. This excision was checked by PCR. Here the original knock-out strain of tar was used as a positive control and a band at 1232 base pairs is expected. If the cassette is removed, a band at 438 base pairs will be visible. Hereby, ten colonies were tested and all have lost their cassette (Figure 1).</p> | ||
</div> | </div> | ||
Revision as of 12:22, 16 September 2015
Results
Double Knockouts
The Centre of Microbial and Plant Genetics (KU Leuven) provided us with three E.coli K-12 strains, with each one representing the knockout for the genes tar, tsr or cheZ. The kanamycin cassette of the tar knock-out strain was removed by the enzyme flippase on pCP20. This excision was checked by PCR. Here the original knock-out strain of tar was used as a positive control and a band at 1232 base pairs is expected. If the cassette is removed, a band at 438 base pairs will be visible. Hereby, ten colonies were tested and all have lost their cassette (Figure 1).
Contact
Address: Celestijnenlaan 200G room 00.08 - 3001 Heverlee
Telephone: +32(0)16 32 73 19
Email: igem@chem.kuleuven.be
