Difference between revisions of "Team:NRP-UEA-Norwich/Parts"
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<tr><td>P-Lac(R0010)_RBS(B0034)_GlgX(K1618026)</td><td>pSB1C3</td><td>Debranching enzyme expression construct</td><td>Removes glycogen branches</td><td><a href="http://parts.igem.org/Part:BBa_K1618026">BBa_K1618026</a></td></tr> | <tr><td>P-Lac(R0010)_RBS(B0034)_GlgX(K1618026)</td><td>pSB1C3</td><td>Debranching enzyme expression construct</td><td>Removes glycogen branches</td><td><a href="http://parts.igem.org/Part:BBa_K1618026">BBa_K1618026</a></td></tr> | ||
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<tr><td>P-Lac(R0010)_RBS(B0034)_Buk</td><td>pSB1C3</td><td>Butyrate kinase expression contruct </td><td> Catalyzes the conversionbutyrate to/from butyryl</td><td><a href="http://parts.igem.org/Part:BBa_K1618012">BBa_K1618012</a></td></tr> | <tr><td>P-Lac(R0010)_RBS(B0034)_Buk</td><td>pSB1C3</td><td>Butyrate kinase expression contruct </td><td> Catalyzes the conversionbutyrate to/from butyryl</td><td><a href="http://parts.igem.org/Part:BBa_K1618012">BBa_K1618012</a></td></tr> | ||
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Revision as of 13:54, 16 September 2015
Parts
BASIC PARTS FOR ENGINEERING BACTERIAL GLYCOGEN
| Part Contents | Backbone | Description | Function | Name in registry |
|---|---|---|---|---|
| GlgX | pSB1C3 | Debranching enzyme (E.coli) | Coding sequence only | BBa_K1618025 |
| GlgB | pSB1C3 | Branching Enzyme (E.coli) | Coding sequence only | BBa_K1618000 |
| MAO | pSB1C3 | Acyl-transferase Candidate 1 | Coding sequence only | BBa_K1618001 |
| Rv3030 | pSB1C3 | Acylation candidate 2 | Coding sequence only | BBa_K1618002 |
| Rv3034c | pSB1C3 | Acylation candidate 3 | Coding sequence only | BBa_K1618003 |
| Rv3037c | pSB1C3 | Acylation candidate 4 | Coding sequence only | BBa_K1618004 |
COMPOUND PARTS FOR ENGINEERING BACTERIAL GLYCOGEN
| Part Contents | Backbone | Description | Function | Name in registry |
|---|---|---|---|---|
| P-Lac(R0010)_RBS(B0034)_GlgX(K1618026) | pSB1C3 | Debranching enzyme expression construct | Removes glycogen branches | BBa_K1618026 |
| P-Lac(R0010)_RBS(B0034)_GlgB(K1618000) | pSB1C3 | Branching enzyme expression construct | Add glycogen branches | BBa_K1618022 |
| P-Lac(R0010)_RBS(B0034)_MAO(K1618001) | pSB1C3 | Acyl-transferase expression construct | Transfers acyl groups to glycogen | BBa_K1618005 |
| P-Lac(R0010)_RBS(B0034)_Rv3030(K1618002) | pSB1C3 | Acyl-transferase expression construct | Transfers acyl groups to glycogen | BBa_K1618007 |
| P-Lac(R0010)_RBS(B0034)_Rv3034c(K1618003) | pSB1C3 | Acyl-transferase expression construct | Transfers acyl groups to glycogen | BBa_K1618008 |
| P-Lac(R0010)_RBS(B0034)_Rv3037c(K1618004) | pSB1C3 | Acyl-transferase expression construct | Transfers acyl groups to glycogen | BBa_K1618009 |
| P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_MAO(K1618001) | pSB1C3 | Branching enzyme + acyl-transferase expression construct | Transfers acyl groups to glycogen | BBa_K1618023 |
| P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3030(K1618002) | pSB1C3 | Branching enzyme + acyl-transferase expression construct | )Transfers acyl groups to glycogen | BBa_K1618023 |
| P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3034c(K1618003) | pSB1C3 | Branching enzyme + acyl-transferase expression construct | Transfers acyl groups to glycogen | BBa_K1618024 |
| P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3037c(K1618004) | pSB1C3 | BBranching enzyme + acyl-transferase expression construct | Transfers acyl groups to glycogen | BBa_K1618027 |
COMPOUND PARTS FROM THE BUTYRATE BIOSYNTHESIS PATHWAY (CODING SEQUNCES WERE FROM Coprococcus sp. L2-50 (DSM 16842)
| Part Contents | Backbone | Description | Function | Name in registry |
|---|---|---|---|---|
| P-Lac(R0010)_RBS(B0034)_Buk | pSB1C3 | Butyrate kinase expression contruct | Catalyzes the conversionbutyrate to/from butyryl | BBa_K1618012 |
| P-Lac(R0010)_RBS(B0034)_PTB | pSB1C3 | Phosphotransbutrylase expression construct | Catalyzes the conversion butyryl to/from butyryl CoA | BBa_K1618013 |
| P-Lac(R0010)_RBS(B0034)_Buk_PTB | pSB1C3 | Butyrate to butyryl CoA pathway | Catalyzes the conversion butyryl to/from butyryl CoA | BBa_K1618020 |
| P-Lac(R0010)_RBS(B0034)_THL | pSB1C3 | Thiolase expression construct | Catalyzes the conversion of Acetyl-CoA to Acetoacetyl-CoA | BBa_K1618014 |
| P-Lac(R0010)_RBS(B0034)_CRO | pSB1C3 | Crotonase expression construct | Catalyzes conversion of3-hydroxybutyryl-CoA to Crotonyl-CoA | BBa_K1618015 |
| P-Lac(R0010)_RBS(B0034)_BHBD | pSB1C3 | β-hydroxybutyryl-CoA dehydrogenase expression | Catalyzes conversion of Acetoacetyl-CoA to 3-hydroxybutyryl-CoA | BBa_K1618016 |
| P-Lac(R0010)_RBS(B0034)_BCD | pSB1C3 | Butyryl-CoA dehydrogenase expression construct | Catalyzes conversion of Crotonyl-CoA to Butyryl-CoA | BBa_K1618017 |
| P-Lac(R0010)_RBS(B0034)_EFTb | pSB1C3 | Electron transfer protein β expression construct | Catalyzes conversion of acetate to butyrate | BBa_K1618018 |
| P-Lac(R0010)_RBS(B0034)_EFTa | pSB1C3 | Electron transfer protein α expression construct | Catalyzes conversion of acetate to butyrate | BBa_K1618019 |
| P-Lac(R0010)_RBS(B0034)_THL-CRO-BHBD | pSB1C3 | Acetyl-CoA to 3-crotonyl-CoA pathway | Catalyzes conversion of Acetyl-CoA to 3-crotonyl-CoA | -------- |
| P-Lac(R0010)_RBS(B0034)_EFTa | pSB1C3 | Crotonyl-CoA to Butyryl-coA pathway | Catalyzes conversion of Crotonyl-CoA to Butyryl-coA | ----------- |
| P-Lac(R0010)_RBS(B0034)_THL-CRO-BHBD-BCD-EFTb-EFTa | pSB1C3 | Acetyl-coA to Butryl-CoA pathway | Catalyzes conversion of Acetyl-coA to Butryl-CoA | BBa_K1618021 |
| P-Lac(R0010)_RBS(B0034)_Buk-PTB-THL-CRO-BHBD-BCD-EFTb-EFTa | pSB1C3 | Acetyl coA to butyrate complete biosynthetic pathway | Catalyzes conversion of Acetyl coA to butyrate | ---------- |
COMPOUND PARTS FOR ENGINEERING PLANT STARCH
| Part Contents | Backbone | Description | Function | Name in registry |
|---|
