Difference between revisions of "Team:NRP-UEA-Norwich/Parts"
| Line 119: | Line 119: | ||
</tr> | </tr> | ||
| − | <tr><td>GlgX</td><td>pSB1C3</td><td>Debranching enzyme <i>(E.coli)</i></td><td>Coding sequence only</td> <td><a href="http://parts.igem.org/Part:BBa_K1618025">BBa_K1618025</a></td></tr> | + | <tr><td>GlgX</td><td>pSB1C3</td><td>Debranching enzyme <i>(E.coli)</i></td><td>Coding sequence only</td> <td><a href="http://parts.igem.org/Part:BBa_K1618025" style = "color: #002bb8;">BBa_K1618025</a></td></tr> |
| − | <tr><td>GlgB</td><td>pSB1C3</td><td>Branching Enzyme <i>(E.coli)</i></td><td>Coding sequence only</td> <td><a href="http://parts.igem.org/Part:BBa_K1618000">BBa_K1618000</a></td></tr> | + | <tr><td>GlgB</td><td>pSB1C3</td><td>Branching Enzyme <i>(E.coli)</i></td><td>Coding sequence only</td> <td><a href="http://parts.igem.org/Part:BBa_K1618000" style = "color: #002bb8;">BBa_K1618000</a></td></tr> |
| − | <tr><td>MAO</td><td>pSB1C3</td><td>Acyl-transferase Candidate 1 </td><td>Coding sequence only</td> <td><a href="http://parts.igem.org/Part:BBa_K1618001">BBa_K1618001</a></td></tr> | + | <tr><td>MAO</td><td>pSB1C3</td><td>Acyl-transferase Candidate 1 </td><td>Coding sequence only</td> <td><a href="http://parts.igem.org/Part:BBa_K1618001" style = "color: #002bb8;">BBa_K1618001</a></td></tr> |
| − | <tr><td>Rv3030</td><td>pSB1C3</td><td>Acylation candidate 2</td><td>Coding sequence only</td><td><a href="http://parts.igem.org/Part:BBa_K1618002">BBa_K1618002</a></td></tr> | + | <tr><td>Rv3030</td><td>pSB1C3</td><td>Acylation candidate 2</td><td>Coding sequence only</td><td><a href="http://parts.igem.org/Part:BBa_K1618002" style = "color: #002bb8;">BBa_K1618002</a></td></tr> |
| − | <tr><td>Rv3034c</td><td>pSB1C3</td><td>Acylation candidate 3</td><td>Coding sequence only</td> <td><a href="http://parts.igem.org/Part:BBa_K1618003">BBa_K1618003</a></td></tr> | + | <tr><td>Rv3034c</td><td>pSB1C3</td><td>Acylation candidate 3</td><td>Coding sequence only</td> <td><a href="http://parts.igem.org/Part:BBa_K1618003" style = "color: #002bb8;">BBa_K1618003</a></td></tr> |
| Line 190: | Line 190: | ||
<tr><td>P-Lac(R0010)_RBS(B0034)_Buk_PTB</td><td>pSB1C3</td><td>Butyrate to butyryl CoA pathway</td><td> Catalyzes the conversion butyryl to/from butyryl CoA</td><td><a href="http://parts.igem.org/Part:BBa_K1618020" style = "color: #002bb8;">BBa_K1618020</a></td></tr> | <tr><td>P-Lac(R0010)_RBS(B0034)_Buk_PTB</td><td>pSB1C3</td><td>Butyrate to butyryl CoA pathway</td><td> Catalyzes the conversion butyryl to/from butyryl CoA</td><td><a href="http://parts.igem.org/Part:BBa_K1618020" style = "color: #002bb8;">BBa_K1618020</a></td></tr> | ||
| − | <tr><td>P-Lac(R0010)_RBS(B0034)_THL</td><td>pSB1C3</td><td>Thiolase expression construct</td><td>Catalyzes the conversion of Acetyl-CoA to Acetoacetyl-CoA </td><td><a href="http://parts.igem.org/Part:BBa_K1618014">BBa_K1618014</a></td></tr> | + | <tr><td>P-Lac(R0010)_RBS(B0034)_THL</td><td>pSB1C3</td><td>Thiolase expression construct</td><td>Catalyzes the conversion of Acetyl-CoA to Acetoacetyl-CoA </td><td><a href="http://parts.igem.org/Part:BBa_K1618014" style = "color: #002bb8;">BBa_K1618014</a></td></tr> |
| − | <tr><td>P-Lac(R0010)_RBS(B0034)_CRO</td><td>pSB1C3</td><td>Crotonase expression construct</td><td>Catalyzes conversion of3-hydroxybutyryl-CoA to Crotonyl-CoA </td><td><a href="http://parts.igem.org/Part:BBa_K1618015">BBa_K1618015</a></td></tr> | + | <tr><td>P-Lac(R0010)_RBS(B0034)_CRO</td><td>pSB1C3</td><td>Crotonase expression construct</td><td>Catalyzes conversion of3-hydroxybutyryl-CoA to Crotonyl-CoA </td><td><a href="http://parts.igem.org/Part:BBa_K1618015" style = "color: #002bb8;">BBa_K1618015</a></td></tr> |
| − | <tr><td>P-Lac(R0010)_RBS(B0034)_BHBD</td><td>pSB1C3</td><td>β-hydroxybutyryl-CoA dehydrogenase expression</td><td>Catalyzes conversion of Acetoacetyl-CoA to 3-hydroxybutyryl-CoA </td><td><a href="http://parts.igem.org/Part:BBa_K1618016">BBa_K1618016</a></td></tr> | + | <tr><td>P-Lac(R0010)_RBS(B0034)_BHBD</td><td>pSB1C3</td><td>β-hydroxybutyryl-CoA dehydrogenase expression</td><td>Catalyzes conversion of Acetoacetyl-CoA to 3-hydroxybutyryl-CoA </td><td><a href="http://parts.igem.org/Part:BBa_K1618016" style = "color: #002bb8;">BBa_K1618016</a></td></tr> |
| − | <tr><td>P-Lac(R0010)_RBS(B0034)_BCD</td><td>pSB1C3</td><td>Butyryl-CoA dehydrogenase expression construct</td><td> Catalyzes conversion of Crotonyl-CoA to Butyryl-CoA </td><td><a href="http://parts.igem.org/Part:BBa_K1618017">BBa_K1618017</a></td></tr> | + | <tr><td>P-Lac(R0010)_RBS(B0034)_BCD</td><td>pSB1C3</td><td>Butyryl-CoA dehydrogenase expression construct</td><td> Catalyzes conversion of Crotonyl-CoA to Butyryl-CoA </td><td><a href="http://parts.igem.org/Part:BBa_K1618017" style = "color: #002bb8;">BBa_K1618017</a></td></tr> |
| − | <tr><td>P-Lac(R0010)_RBS(B0034)_EFTb</td><td>pSB1C3</td><td>Electron transfer protein β expression construct</td><td>Catalyzes conversion of acetate to butyrate</td><td><a href="http://parts.igem.org/Part:BBa_K1618018">BBa_K1618018</a></td></tr> | + | <tr><td>P-Lac(R0010)_RBS(B0034)_EFTb</td><td>pSB1C3</td><td>Electron transfer protein β expression construct</td><td>Catalyzes conversion of acetate to butyrate</td><td><a href="http://parts.igem.org/Part:BBa_K1618018" style = "color: #002bb8;">BBa_K1618018</a></td></tr> |
| − | <tr><td>P-Lac(R0010)_RBS(B0034)_EFTa</td><td>pSB1C3</td><td>Electron transfer protein α expression construct</td><td>Catalyzes conversion of acetate to butyrate</td><td><a href="http://parts.igem.org/Part:BBa_K1618019">BBa_K1618019</a></td></tr> | + | <tr><td>P-Lac(R0010)_RBS(B0034)_EFTa</td><td>pSB1C3</td><td>Electron transfer protein α expression construct</td><td>Catalyzes conversion of acetate to butyrate</td><td><a href="http://parts.igem.org/Part:BBa_K1618019" style = "color: #002bb8;">BBa_K1618019</a></td></tr> |
| − | <tr><td>P-Lac(R0010)_RBS(B0034)_THL-CRO-BHBD</td><td>pSB1C3</td><td>Acetyl-CoA to 3-crotonyl-CoA pathway</td><td>Catalyzes conversion of Acetyl-CoA to 3-crotonyl-CoA</td><td>--------</a></td></tr> | + | <tr><td>P-Lac(R0010)_RBS(B0034)_THL-CRO-BHBD</td><td>pSB1C3</td><td>Acetyl-CoA to 3-crotonyl-CoA pathway</td><td>Catalyzes conversion of Acetyl-CoA to 3-crotonyl-CoA</td><td><a href = "" style = "color: #002bb8;">--------</a></td></tr> |
| − | <tr><td>P-Lac(R0010)_RBS(B0034)_EFTa</td><td>pSB1C3</td><td>Crotonyl-CoA to Butyryl-coA pathway</td><td>Catalyzes conversion of Crotonyl-CoA to Butyryl-coA</td><td>-----------</a></td></tr> | + | <tr><td>P-Lac(R0010)_RBS(B0034)_EFTa</td><td>pSB1C3</td><td>Crotonyl-CoA to Butyryl-coA pathway</td><td>Catalyzes conversion of Crotonyl-CoA to Butyryl-coA</td><td><a href="" style = "color: #002bb8;">-----------</a></td></tr> |
| − | <tr><td>P-Lac(R0010)_RBS(B0034)_THL-CRO-BHBD-BCD-EFTb-EFTa</td><td>pSB1C3</td><td>Acetyl-coA to Butryl-CoA pathway</td><td>Catalyzes conversion of Acetyl-coA to Butryl-CoA </td><td><a href="http://parts.igem.org/Part:BBa_K1618021">BBa_K1618021</a></td></tr> | + | <tr><td>P-Lac(R0010)_RBS(B0034)_THL-CRO-BHBD-BCD-EFTb-EFTa</td><td>pSB1C3</td><td>Acetyl-coA to Butryl-CoA pathway</td><td>Catalyzes conversion of Acetyl-coA to Butryl-CoA </td><td><a href="http://parts.igem.org/Part:BBa_K1618021" style = "color: #002bb8;">BBa_K1618021</a></td></tr> |
| Line 232: | Line 232: | ||
| − | <tr><td>P-35s(K1467101)_TP(K1618037):MAO(K1618001)_T-35s(BBa_K1618037)</td><td>pSB1C3*</td><td>Acylation candidate 1 expression construct</td><td>Transfers acyl groups to starch</td><td><a href="http://parts.igem.org/Part:BBa_K1618033">BBa_K1618033</a></td></tr> | + | <tr><td>P-35s(K1467101)_TP(K1618037):MAO(K1618001)_T-35s(BBa_K1618037)</td><td>pSB1C3*</td><td>Acylation candidate 1 expression construct</td><td>Transfers acyl groups to starch</td><td><a href="http://parts.igem.org/Part:BBa_K1618033" style = "color: #002bb8;">BBa_K1618033</a></td></tr> |
| − | <tr><td>P-35s(K1467101)_TP(K1618037):Rv3030(K1618002)_T-35s(BBa_K1618037)</td><td>pSB1C3*</td><td>Acylation candidate 2 expression construct</td><td>Transfers acyl groups to starch</td><td><a href="http://parts.igem.org/Part:BBa_K1618034">BBa_K1618034</a></td></tr> | + | <tr><td>P-35s(K1467101)_TP(K1618037):Rv3030(K1618002)_T-35s(BBa_K1618037)</td><td>pSB1C3*</td><td>Acylation candidate 2 expression construct</td><td>Transfers acyl groups to starch</td><td><a href="http://parts.igem.org/Part:BBa_K1618034" style = "color: #002bb8;">BBa_K1618034</a></td></tr> |
| − | <tr><td>P-35s(K1467101)_TP(K1618037):Rv3034c(K1618003)_T-35s(BBa_K1618037)</td><td>pSB1C3*</td><td>Acylation candidate 3 expression construct</td><td>Transfers acyl groups to starch</td><td><a href="http://parts.igem.org/Part:BBa_K1618035">BBa_K1618035</a></td></tr> | + | <tr><td>P-35s(K1467101)_TP(K1618037):Rv3034c(K1618003)_T-35s(BBa_K1618037)</td><td>pSB1C3*</td><td>Acylation candidate 3 expression construct</td><td>Transfers acyl groups to starch</td><td><a href="http://parts.igem.org/Part:BBa_K1618035" style = "color: #002bb8;">BBa_K1618035</a></td></tr> |
| − | <tr><td>P-35s(K1467101)_TP(K1618037):Rv3037c(K1618004)_T-35s(BBa_K1618037)</td><td>pSB1C3*</td><td>Acylation candidate 4 expression construct</td><td>Transfers acyl groups to starch</td><td><a href="http://parts.igem.org/Part:BBa_K1618036">BBa_K1618036</a></td></tr> | + | <tr><td>P-35s(K1467101)_TP(K1618037):Rv3037c(K1618004)_T-35s(BBa_K1618037)</td><td>pSB1C3*</td><td>Acylation candidate 4 expression construct</td><td>Transfers acyl groups to starch</td><td><a href="http://parts.igem.org/Part:BBa_K1618036" style = "color: #002bb8;">BBa_K1618036</a></td></tr> |
| − | <tr><td>P-35s(K1467101)_TP(K1618037):MAO(K1618001):GFP(K1467204)_T-35s(BBa_K1618037)</td><td>pSB1C3*</td><td>Acylation candidate 1 -fused to GFP -expression construct </td><td>Check localization in plastid of candidate gene</td><td><a href="http://parts.igem.org/Part:BBa_K1618029">BBa_K1618029</a></td></tr> | + | <tr><td>P-35s(K1467101)_TP(K1618037):MAO(K1618001):GFP(K1467204)_T-35s(BBa_K1618037)</td><td>pSB1C3*</td><td>Acylation candidate 1 -fused to GFP -expression construct </td><td>Check localization in plastid of candidate gene</td><td><a href="http://parts.igem.org/Part:BBa_K1618029" style = "color: #002bb8;">BBa_K1618029</a></td></tr> |
| − | <tr><td>P-35s(K1467101)_TP(K1618037):Rv3030(K1618002):GFP(K1467204_T-35s(BBa_K1618037)</td><td>pSB1C3*</td><td>Acylation candidate 2 -fused to GFP -expression construct </td><td>Check localization in plastid of candidate gene</td><td><a href="http://parts.igem.org/Part:BBa_K1618030">BBa_K1618030</a></td></tr> | + | <tr><td>P-35s(K1467101)_TP(K1618037):Rv3030(K1618002):GFP(K1467204_T-35s(BBa_K1618037)</td><td>pSB1C3*</td><td>Acylation candidate 2 -fused to GFP -expression construct </td><td>Check localization in plastid of candidate gene</td><td><a href="http://parts.igem.org/Part:BBa_K1618030" style = "color: #002bb8;">BBa_K1618030</a></td></tr> |
| − | <tr><td>P-35s(K1467101)_TP(K1618037):Rv3034c(K1618003):GFP(K1467204_T-35s(BBa_K1618037)</td><td>pSB1C3*</td><td>Acylation candidate 3 -fused to GFP -expression construct </td><td>Check localization in plastid of candidate gene</td><td><a href="http://parts.igem.org/Part:BBa_K1618031">BBa_K1618031</a></td></tr> | + | <tr><td>P-35s(K1467101)_TP(K1618037):Rv3034c(K1618003):GFP(K1467204_T-35s(BBa_K1618037)</td><td>pSB1C3*</td><td>Acylation candidate 3 -fused to GFP -expression construct </td><td>Check localization in plastid of candidate gene</td><td><a href="http://parts.igem.org/Part:BBa_K1618031" style = "color: #002bb8;">BBa_K1618031</a></td></tr> |
| − | <tr><td>P-35s(K1467101)_TP(K1618037):Rv3037c(K1618004):GFP(K1467204_T-35s(BBa_K1618037)</td><td>pSB1C3*</td><td>Acylation candidate 4 -fused to GFP -expression construct </td><td>Check localization in plastid of candidate gene</td><td><a href="http://parts.igem.org/Part:BBa_K1618032">BBa_K1618032</a></td></tr> | + | <tr><td>P-35s(K1467101)_TP(K1618037):Rv3037c(K1618004):GFP(K1467204_T-35s(BBa_K1618037)</td><td>pSB1C3*</td><td>Acylation candidate 4 -fused to GFP -expression construct </td><td>Check localization in plastid of candidate gene</td><td><a href="http://parts.igem.org/Part:BBa_K1618032" style = "color: #002bb8;">BBa_K1618032</a></td></tr> |
| − | <tr><td>35s Terminator</td><td>pSB1C3**</td><td>Eukaryotic terminator from the 35s gene (Cauliflower Mosaic Virus)</td><td>terminates transcription in plant cells</td><td><a href="http://parts.igem.org/Part:BBa_K1618037">BBa_K1618037</a></td></tr> | + | <tr><td>35s Terminator</td><td>pSB1C3**</td><td>Eukaryotic terminator from the 35s gene (Cauliflower Mosaic Virus)</td><td>terminates transcription in plant cells</td><td><a href="http://parts.igem.org/Part:BBa_K1618037" style = "color: #002bb8;">BBa_K1618037</a></td></tr> |
| − | <tr><td>Chloroplast Transit Peptide</td><td>pSB1C3***</td><td>Synthetic plastid transit peptide form the consensus of multiple RuBisCo Small Subunit genes</td><td>In an N-terminal fusion, this peptide directs proteins to the plant plastid.</td><td><a href="http://parts.igem.org/Part:BBa_K1618028">BBa_K1618028</a></td></tr> | + | <tr><td>Chloroplast Transit Peptide</td><td>pSB1C3***</td><td>Synthetic plastid transit peptide form the consensus of multiple RuBisCo Small Subunit genes</td><td>In an N-terminal fusion, this peptide directs proteins to the plant plastid.</td><td><a href="http://parts.igem.org/Part:BBa_K1618028" style = "color: #002bb8;">BBa_K1618028</a></td></tr> |
</table> | </table> | ||
<div class="space10"></div> | <div class="space10"></div> | ||
| − | <p>* This was convered to a standard BioBrick by cloning into the Mo-Flipper Plasmid K1467400 (complete transcritional unit flipper) made by the NRP UEA 2014 team. These parts were tested by cloning into a binary plasmid vector backbone prior to delivery to plants leaves using | + | <p>* This was convered to a standard BioBrick by cloning into the Mo-Flipper Plasmid K1467400 (complete transcritional unit flipper) made by the NRP UEA 2014 team. These parts were tested by cloning into a binary plasmid vector backbone prior to delivery to plants leaves using Agrobacterium-tumefaciens mediates trasnfection.</p> |
<p>** This was convered to a standard BioBrick by cloning into the Mo-Flipper Plasmid K1467300 (terminator flipper) made by the NRP UEA 2014 team.</p> | <p>** This was convered to a standard BioBrick by cloning into the Mo-Flipper Plasmid K1467300 (terminator flipper) made by the NRP UEA 2014 team.</p> | ||
Revision as of 14:16, 16 September 2015
Parts
BASIC PARTS FOR ENGINEERING BACTERIAL GLYCOGEN
| Part Contents | Backbone | Description | Function | Name in registry |
|---|---|---|---|---|
| GlgX | pSB1C3 | Debranching enzyme (E.coli) | Coding sequence only | BBa_K1618025 |
| GlgB | pSB1C3 | Branching Enzyme (E.coli) | Coding sequence only | BBa_K1618000 |
| MAO | pSB1C3 | Acyl-transferase Candidate 1 | Coding sequence only | BBa_K1618001 |
| Rv3030 | pSB1C3 | Acylation candidate 2 | Coding sequence only | BBa_K1618002 |
| Rv3034c | pSB1C3 | Acylation candidate 3 | Coding sequence only | BBa_K1618003 |
| Rv3037c | pSB1C3 | Acylation candidate 4 | Coding sequence only | BBa_K1618004 |
COMPOUND PARTS FOR ENGINEERING BACTERIAL GLYCOGEN
| Part Contents | Backbone | Description | Function | Name in registry |
|---|---|---|---|---|
| P-Lac(R0010)_RBS(B0034)_GlgX(K1618026) | pSB1C3 | Debranching enzyme expression construct | Removes glycogen branches | BBa_K1618026 |
| P-Lac(R0010)_RBS(B0034)_GlgB(K1618000) | pSB1C3 | Branching enzyme expression construct | Add glycogen branches | BBa_K1618022 |
| P-Lac(R0010)_RBS(B0034)_MAO(K1618001) | pSB1C3 | Acyl-transferase expression construct | Transfers acyl groups to glycogen | BBa_K1618005 |
| P-Lac(R0010)_RBS(B0034)_Rv3030(K1618002) | pSB1C3 | Acyl-transferase expression construct | Transfers acyl groups to glycogen | BBa_K1618007 |
| P-Lac(R0010)_RBS(B0034)_Rv3034c(K1618003) | pSB1C3 | Acyl-transferase expression construct | Transfers acyl groups to glycogen | BBa_K1618008 |
| P-Lac(R0010)_RBS(B0034)_Rv3037c(K1618004) | pSB1C3 | Acyl-transferase expression construct | Transfers acyl groups to glycogen | BBa_K1618009 |
| P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_MAO(K1618001) | pSB1C3 | Branching enzyme + acyl-transferase expression construct | Transfers acyl groups to glycogen | BBa_K1618023 |
| P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3030(K1618002) | pSB1C3 | Branching enzyme + acyl-transferase expression construct | Transfers acyl groups to glycogen | BBa_K1618023 |
| P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3034c(K1618003) | pSB1C3 | Branching enzyme + acyl-transferase expression construct | Transfers acyl groups to glycogen | BBa_K1618024 |
| P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3037c(K1618004) | pSB1C3 | BBranching enzyme + acyl-transferase expression construct | Transfers acyl groups to glycogen | BBa_K1618027 |
COMPOUND PARTS FROM THE BUTYRATE BIOSYNTHESIS PATHWAY (CODING SEQUNCES WERE FROM Coprococcus sp. L2-50 (DSM 16842)
| Part Contents | Backbone | Description | Function | Name in registry |
|---|---|---|---|---|
| P-Lac(R0010)_RBS(B0034)_Buk | pSB1C3 | Butyrate kinase expression contruct | Catalyzes the conversionbutyrate to/from butyryl | BBa_K1618012 |
| P-Lac(R0010)_RBS(B0034)_PTB | pSB1C3 | Phosphotransbutrylase expression construct | Catalyzes the conversion butyryl to/from butyryl CoA | BBa_K1618013 |
| P-Lac(R0010)_RBS(B0034)_Buk_PTB | pSB1C3 | Butyrate to butyryl CoA pathway | Catalyzes the conversion butyryl to/from butyryl CoA | BBa_K1618020 |
| P-Lac(R0010)_RBS(B0034)_THL | pSB1C3 | Thiolase expression construct | Catalyzes the conversion of Acetyl-CoA to Acetoacetyl-CoA | BBa_K1618014 |
| P-Lac(R0010)_RBS(B0034)_CRO | pSB1C3 | Crotonase expression construct | Catalyzes conversion of3-hydroxybutyryl-CoA to Crotonyl-CoA | BBa_K1618015 |
| P-Lac(R0010)_RBS(B0034)_BHBD | pSB1C3 | β-hydroxybutyryl-CoA dehydrogenase expression | Catalyzes conversion of Acetoacetyl-CoA to 3-hydroxybutyryl-CoA | BBa_K1618016 |
| P-Lac(R0010)_RBS(B0034)_BCD | pSB1C3 | Butyryl-CoA dehydrogenase expression construct | Catalyzes conversion of Crotonyl-CoA to Butyryl-CoA | BBa_K1618017 |
| P-Lac(R0010)_RBS(B0034)_EFTb | pSB1C3 | Electron transfer protein β expression construct | Catalyzes conversion of acetate to butyrate | BBa_K1618018 |
| P-Lac(R0010)_RBS(B0034)_EFTa | pSB1C3 | Electron transfer protein α expression construct | Catalyzes conversion of acetate to butyrate | BBa_K1618019 |
| P-Lac(R0010)_RBS(B0034)_THL-CRO-BHBD | pSB1C3 | Acetyl-CoA to 3-crotonyl-CoA pathway | Catalyzes conversion of Acetyl-CoA to 3-crotonyl-CoA | -------- |
| P-Lac(R0010)_RBS(B0034)_EFTa | pSB1C3 | Crotonyl-CoA to Butyryl-coA pathway | Catalyzes conversion of Crotonyl-CoA to Butyryl-coA | ----------- |
| P-Lac(R0010)_RBS(B0034)_THL-CRO-BHBD-BCD-EFTb-EFTa | pSB1C3 | Acetyl-coA to Butryl-CoA pathway | Catalyzes conversion of Acetyl-coA to Butryl-CoA | BBa_K1618021 |
| P-Lac(R0010)_RBS(B0034)_Buk-PTB-THL-CRO-BHBD-BCD-EFTb-EFTa | pSB1C3 | Acetyl coA to butyrate complete biosynthetic pathway | Catalyzes conversion of Acetyl coA to butyrate | ---------- |
COMPOUND PARTS FOR ENGINEERING PLANT STARCH
| Part Contents | Backbone | Description | Function | Name in registry |
|---|---|---|---|---|
| P-35s(K1467101)_TP(K1618037):MAO(K1618001)_T-35s(BBa_K1618037) | pSB1C3* | Acylation candidate 1 expression construct | Transfers acyl groups to starch | BBa_K1618033 |
| P-35s(K1467101)_TP(K1618037):Rv3030(K1618002)_T-35s(BBa_K1618037) | pSB1C3* | Acylation candidate 2 expression construct | Transfers acyl groups to starch | BBa_K1618034 |
| P-35s(K1467101)_TP(K1618037):Rv3034c(K1618003)_T-35s(BBa_K1618037) | pSB1C3* | Acylation candidate 3 expression construct | Transfers acyl groups to starch | BBa_K1618035 |
| P-35s(K1467101)_TP(K1618037):Rv3037c(K1618004)_T-35s(BBa_K1618037) | pSB1C3* | Acylation candidate 4 expression construct | Transfers acyl groups to starch | BBa_K1618036 |
| P-35s(K1467101)_TP(K1618037):MAO(K1618001):GFP(K1467204)_T-35s(BBa_K1618037) | pSB1C3* | Acylation candidate 1 -fused to GFP -expression construct | Check localization in plastid of candidate gene | BBa_K1618029 |
| P-35s(K1467101)_TP(K1618037):Rv3030(K1618002):GFP(K1467204_T-35s(BBa_K1618037) | pSB1C3* | Acylation candidate 2 -fused to GFP -expression construct | Check localization in plastid of candidate gene | BBa_K1618030 |
| P-35s(K1467101)_TP(K1618037):Rv3034c(K1618003):GFP(K1467204_T-35s(BBa_K1618037) | pSB1C3* | Acylation candidate 3 -fused to GFP -expression construct | Check localization in plastid of candidate gene | BBa_K1618031 |
| P-35s(K1467101)_TP(K1618037):Rv3037c(K1618004):GFP(K1467204_T-35s(BBa_K1618037) | pSB1C3* | Acylation candidate 4 -fused to GFP -expression construct | Check localization in plastid of candidate gene | BBa_K1618032 |
| 35s Terminator | pSB1C3** | Eukaryotic terminator from the 35s gene (Cauliflower Mosaic Virus) | terminates transcription in plant cells | BBa_K1618037 |
| Chloroplast Transit Peptide | pSB1C3*** | Synthetic plastid transit peptide form the consensus of multiple RuBisCo Small Subunit genes | In an N-terminal fusion, this peptide directs proteins to the plant plastid. | BBa_K1618028 |
* This was convered to a standard BioBrick by cloning into the Mo-Flipper Plasmid K1467400 (complete transcritional unit flipper) made by the NRP UEA 2014 team. These parts were tested by cloning into a binary plasmid vector backbone prior to delivery to plants leaves using Agrobacterium-tumefaciens mediates trasnfection.
** This was convered to a standard BioBrick by cloning into the Mo-Flipper Plasmid K1467300 (terminator flipper) made by the NRP UEA 2014 team.
*** This is assembled in a Golden Gate Universal Acceptor Plasmid (pUAP1) in the pSB1C3 backbone according to RFC 106. It is a Golden Gate part in the plant standard syntax, not a BioBrick.
