Difference between revisions of "Team:Toulouse/Design"

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TrApiColi has been designed in order to take into account ethical  
 
TrApiColi has been designed in order to take into account ethical  
 
reflection, safety and ease of use for beekeepers. The use of  
 
reflection, safety and ease of use for beekeepers. The use of  
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genetically modified organisms in a field and more, associated with  
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genetically modified organisms in a field, and because our project is associated with  
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edible products underlies both law enforcement and applying of
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edible products, underlies both applying of regulations and public interest.  
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regulations and public perception account about it.  
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In this context, as explain above, we searched a solution being able to isolate our engineered bacteria from  
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In this context, we designed a device being able to isolate our engineered bacteria from  
+
  the environment, but allowing its growth, metabolism and gas diffusion. We found the  
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  the environment, but allowing its growth and metabolism. We found the  
+
 
  project of the iGEM Groeningen 2012 team which used the polymer TPX® in  
 
  project of the iGEM Groeningen 2012 team which used the polymer TPX® in  
 
  order to contain their bacteria separated of the meat [1]. Thus, we  
 
  order to contain their bacteria separated of the meat [1]. Thus, we  
−
  decided to ask them TPX® characteristics and their supplier. We obtained
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  decided to ask them TPX® characteristics. We finally contacted the TPX® supplier. We obtained
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  some samples of TPX® by contacting the company MitsuiChemicals.  
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  some samples of TPX® by contacting the company MitsuiChemicals. TPX®, Polymethylpentene (4-methylpentene-1 based polyolefin, Mitsui Chemicals, Inc.), is a polymer.
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 +
 
 
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Revision as of 17:52, 16 September 2015

iGEM Toulouse 2015

Device


Trap Construction

TPX Bag

TrApiColi has been designed in order to take into account ethical reflection, safety and ease of use for beekeepers. The use of genetically modified organisms in a field, and because our project is associated with edible products, underlies both applying of regulations and public interest. In this context, as explain above, we searched a solution being able to isolate our engineered bacteria from the environment, but allowing its growth, metabolism and gas diffusion. We found the project of the iGEM Groeningen 2012 team which used the polymer TPX® in order to contain their bacteria separated of the meat [1]. Thus, we decided to ask them TPX® characteristics. We finally contacted the TPX® supplier. We obtained some samples of TPX® by contacting the company MitsuiChemicals. TPX®, Polymethylpentene (4-methylpentene-1 based polyolefin, Mitsui Chemicals, Inc.), is a polymer.

To check if our device can be safe and feasible, several tests have been performed:

  • Safety test: Impermeability of bacteria through the bag of TPX®
  • Gas diffusion tests: Permeability of butyric acid and formic acid through the bag of TPX®
  • Growth tests in TPX®: Culture of the strain E. coli BW25113 can grow in a TPX® bag (ie. in microaerobic conditions, without agitation and miming batch culture condition), as it would be in the field
  • Bacterial survival over 15 days in microaerobic condition
  • Carbone source test: choice of Carbon source to produce acids during 10 days
  • Acid toxicity on E. coli

References

  • [1] REFERENCE 1 Vers la page Groeningen 2012
  • [2] REFERENCE 2 AVEC UN LIEN See more
  • [3] REFERENCE 2 AVEC UN LIEN qui ouvre dans une nouvelle fenêtre See more