Difference between revisions of "Team:Warwick/Collaborations"

Glasgow Collaboration

While we were conducting our usual experiments, we noticed we had a lot of errors concerning gel extraction using the gels we had made with ethidium bromide. At the UK iGEM team meetup, we got to talking with Glasgow's team to discover they could have a novel method of working around our problem; specifically they had made their own fluorescent tag that could be used for gel electrophoresis. Glasgow was kind enough to send us some of their fluorescent dye for us to use in our next gel extraction.

Manchester-Graz Collaboration

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NTNU Modelling Collaboration

After we came up with a model of DNA Origami arms and the sequences for them and how they would bond we wanted to model the probability of the E.coli arms forming fully and how this probability would change as length of the arms increased. We came up with a basic model but thought it would be beneficial if we sought outside help with the mathematics.
We got in contact with NTNU to ask for assistance and they came back with a stochastic method for calculating said probabilities. After discussing the problem further we came up with various equations such as S_i= Σ_j (K_i,j / Σ_j K_i,j ) log K_i,j / E_j[K_i,j], where S is how well a zinc finger binds to an arm and P_i = S_i / W (S_i) where P is the probability of formation for that zinc finger arm.

Oxford Collaboration

One part of our project was create a DNA origami glue using a biobrick part from the distributed kit, specifically part BBa_K314110. Once we had designed it, we decided to collaborate with Oxford's iGEM team to help get it made.
Oxford's team sped up he production of our DNA origami by conducting a PCR using primers we had designed and sent to them to create seven PCR products, six of which would be directly used to create the DNA origami.
Once we received the PCR products, we combined and annealed them to form DNA origami structures, which could then be viewed under an electron micrograph.

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 			<h5 class="sidebartitle">Glasgow Collaboration</h5>
 			<p><p style="float: left;"><img src="https://static.igem.org/mediawiki/2015/a/ae/2015-Glasgow-sticker.png" height="120px" width="120px" border="1px"></p>
-			 ADD DESCRIPTION
+			 While we were conducting our usual experiments, we noticed we had a lot of errors concerning gel extraction using the gels we had made with ethidium bromide. At the UK iGEM team meetup, we got to talking with Glasgow's team to discover they could have a novel method of working around our problem; specifically they had made their own fluorescent tag that could be used for gel electrophoresis. Glasgow was kind enough to send us some of their fluorescent dye for us to use in our next gel extraction.
 			</p>
 		</div>
@@ Line 47: / Line 47: @@
 			<h5 class="sidebartitle">Oxford Collaboration</h5>
 			<p><p style="float: left;"><img src="https://static.igem.org/mediawiki/2015/7/7c/Warwickocfordlogo.png" height="120px" width="120px" border="1px"></p>
-			 One part of our project was create DNA Origami arms using a part from the distributed kit. Once we had the design made, we decided to collaborate with Oxford to help get it made.
+			 One part of our project was create a DNA origami glue using a biobrick part from the distributed kit, specifically part BBa_K314110. Once we had designed it, we decided to collaborate with Oxford's iGEM team to help get it made.
-<br> We worked with them to PCR a part we had designed using primers we had sequenced. This allowed us to use our time more efficiently and begin work on the second phase of the project.
+<br> Oxford's team sped up he production of our DNA origami by conducting a PCR using primers we had designed and sent to them to create seven PCR products, six of which would be directly used to create the DNA origami.
-<br> Once we got the parts back we combined them and annealed to form DNA Origami shapes, which were then viewed under an electron microscope.
+<br> Once we received the PCR products, we combined and annealed them to form DNA origami structures, which could then be viewed under an electron micrograph.
 			</p>
 		</div>

Difference between revisions of "Team:Warwick/Collaborations"

Revision as of 18:52, 16 September 2015

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