July 1

Lab Work

Preparation

by Johan

Plasmid Rehydratation :

by Johan Measure fluorescence : I13504 J23117 J23106 J23101

For our project : R0051 J13602 J13522 C0040

Preparation of liquid culture of DH5α with K115017

K115017 5mL LB + 5uL Cm (20mg/mL)

Competente cells preparation

by Seong Koo, Pauline, Johan

Preparation competente cells DH5α with CaCl2 cf:protocol

15mL competente cells Aliquot the solution in chilled 1.5mL microcentrifuge tubes (500uL/tube) and frozen and store at -70°C

Preparation LB Plate

by Seong Koo and Pauline

22 Plates LB + Cm (20uL/mL) 12 Plates LB + Amp (100uL/mL)

Members present:

• Instructors : Alice.
• Students : Johan, Seong Koo, Pauline.

Back to the calendar