Difference between revisions of "Team:Wellesley TheTech/Project/Wetlab"

Line 43: Line 43:
 
<div id="pageContent">
 
<div id="pageContent">
 
<h1>WETLAB COMPONENT</h1>
 
<h1>WETLAB COMPONENT</h1>
 +
<br>
 +
<div class="floated_img">
 +
    <img src="https://static.igem.org/mediawiki/2015/1/15/Wellesley_TheTech_Science_wetlab1.png" width="350" height="200" alt="Wet lab prototype 1"> <img src="https://static.igem.org/mediawiki/2015/a/af/Wellesley_TheTech_Science_wetlab2.png" width="350" height="200" alt="Wet lab prototype 2">
 +
    <p>Description of above image</p>
 +
 +
</div>
 +
 
<hr>
 
<hr>
 
<h3>Goal</h3>
 
<h3>Goal</h3>

Revision as of 23:05, 16 September 2015

WETLAB COMPONENT


Wet lab prototype 1 Wet lab prototype 2

Description of above image

Goal

The goal of the wetlab component of BacPack is to connect the digital experience to a hands-on, lab-based one. In the digital activity visitors explore designing bacteria to have a variety of new, useful functions for helping humans survive in different extreme environments. In the wetlab activity visitors get try their hands at the same real-world process of engineering a bacteria to have a new property of their choosing.

Activity Design Outline

Visitors transform a living bacteria to make it do something new:
-5 DNA options that change the physical appearance of bacteria in different ways
-Each ‘color’ represents a bacteria function that can provide one of the 5 needs on Mars
-Visitors pick the DNA they want to use and transform e.coli with it
-They can see a photo of their grown ‘mars bacteria’ several days later

Development

Plasmids:
Selected 5 bioBricks from the iGEM distribution kit:
-Lux Operon (BBa_K325909) - Bioluminescence pathway
amilGFP (BBa_K592010) - Yellow chromoprotein
aeBlue (BBa_K864401) - Blue chromoprotein
eforRed (BBa_K592012) - Red/pink chromoprotein
asPink (BBa_K1033933) - Pink/purple chromoprotein