TEAM WE ARE SIREN
Li Yuehuan
A sophomore bioscience student. She helped design verify parts of the module.
Wu Zekai
A sophomore biology student. Designed a part of biobrick of photoinduction system. and took part in some experiments of verifing the toxicity of Bace 16.
Liu Longping
A sophomore student who has worked on how to promote the expression rate of rMpL as well as designed the toxic test on nematode
Attribution
| Team Member | Attributions |
|---|---|
| Cao Yalei | Graphic Design(Design of logo, banner, poster, jersey and souvenir) |
| Cao Yufei | Wet lab group leader(parts design for expressing lemonene in E. coli and coresponding experiments) |
| Chen Zhiyao | Wiki construction(database construction; UI design) Human practice |
| Dai Yuanyi | Daily activities arrangement and personnel management Wet lab group leader(biobricks construction, Bace16 expression and testification) Human practice |
| Feng Xiaofei | Wet lab experiments(limonene synthesis) Human practice |
| Gao Beichen | Team leader Wet lab group leader（biobricks construction, nematocidal protein expression, rMpL's toxicity identification Human practice |
| Lao Kejing | Wet lab experiments(plasmid purification; SDS-PAGE; nematodes synchronization) Helping Beijing Institute of Technology with extracting membrane proteins, NhaA and NhaB |
| Lee | Graphic Design( website design, logo of the team and T-shirt) |
| Li Cheng | Modeling leader(design of the device 2.0 ; parts improving) Wiki Graphic design |
| Li Jia | Wet lab group leader(chemical attractants' function testification, limonene and GPPs expression, C.elegans cultivation ) Human practice |
| Li Yuehuan | Wet lab experiments(rMpL verification) |
| Liu Longping | Wet lab experiments(exploraton of how to promote the expression rate of protein rMpL; design of the toxic test) |
| Liu Xinyu | Wet lab experiments(limonene group and other parts' experiments) Human practice |
| Ma Hongfei | Wet lab experiments(GC-MS of limonene ) Modeling(device 1.0 design and creation) |
| Peng Chengfei | Wiki construction(database construction, deployment and operation, Wiki frame construction) |
| Tang Meng | Human practice project leader(activities planning and organization) Wet lab experiments |
| Wang Ran | Translation Human practice |
| Wang Yingbing | Wet lab experiments(Bace16 group work) |
| Wu Zekai | Wet lab experiments(photoinduced system design; Bace 16 functional verification) |
| Xiao Shusheng | Literature reading Human practice |
| Xu Lu | Graphic design leader（mainly on poster and PPT design） |
| Yin Yuan | Modeling(proposing the solving ideas; searching the data) |
| Yuan Qiuyue | Modeling(nematode movement simulation;model of diffusion of the attraction) Human practice |
| Zhang Jiajun | Wiki leader Rear services Human practice |
| Zhang Tianqi | Rear services Wet lab experiments(validating limonene's attracting effects on nematodes) |
| Zhao Jingyu | Wet lab experiments(rMPL expression) |
| Zhao Xueting | Modeling(solving problems like how to put equipment on farmland; paper writing; translation) |
Collaboration
Our modeling can be divided into four parts. Firstly, in order to enable our project to be applied in life, we design a device placed in soil, which can attract and kill nematodes by modified engineering bacteria inside. Secondly, assuming there is a farmland, we take advantage of nematodes’ movement analogue simulation to find the best position where the device should be placed and the best concentration of attracting substance. Thirdly, according to this concentration, we obtain the best size of the device by calculating. At last, we establish a database to enlarge applied range of our method to kill other pests. We would appreciate that new synthetic biological and environment-friendly methods can be shared and improved with the science researchers all over the world.
Acknowledgement
Thanks:
Prof. Zhu Xudong, Prof. Yang Dong and Prof. Xiang Benqiong
Offered us much precious advice on the project.
Hao Xiaoran, Chi Xiaodong, Huo Liang, Ai Ying and Wang Xuan
Offered us advice on our experiments and helped us solve problems.
The College of Chemistry, Beijing Normal University
Helped us do GC-MS.
Prof. Yang Chonglin of Chinese Academy of Sciences
Offered us C.elegens and taught us how to cultivate them.
Prof. Li Hongmei of Department of Plant Pathology，Nanjing Agricultural University
Helped us to do Species identification of nematodes.
BIT-China & ZJU-China & FAFU-CHINA
Offered us some helpful advice and we also had a collaboration together.
Agricultural Service Center of Ceyu, Hebei Province
Helped us learn more information about the damage caused by local nematodes and villager’s attitude towards agricultural insecticide.
Shang Zhong National Middle School of Guizhou Province
Supported us in our voluntary education.
National Chiao Tung University &Peking University
Provided us opportunities to communicate with other teams.
Feng Xiaofei and her family in Hebei Province
Helped us get soybeans samples and did a survey in the locality.
Prof. Wang Yingdian
Discussed the bright future of our project with us.
Sponsors
Beijing Normal University (BNU), is a public research university located in Beijing with strong emphasis on basic disciplines of humanities and sciences. It is one of the oldest and most prestigious universities in China. Our school supported us to our iGEM Journey and provided us adequate resources to complete the competition. Students of different colleges in our school took part in our team, and all these colleges supported us very much.
