Difference between revisions of "Team:Jilin China/Design"

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<p> First, we adjusted prior vector by adding a gene called T7 RNA polymerase(Figure 2). What’s more, we used pET vectors to express two key enzymes, and constructed such a vector(Figure 3).</p>
 
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<p> Finally, when T7 polymerase is expressed, Vector pET-DUET can express PADH and FDH. In this way, the whole system can be induced by formaldehyde and express PADH and FDH to degrade it.</p>
 
 
<div align="center"> <img src="https://static.igem.org/mediawiki/2015/3/37/Team_Jilin_China_project2.png" height="150px"> </div>
 
<div align="center"> <img src="https://static.igem.org/mediawiki/2015/3/37/Team_Jilin_China_project2.png" height="150px"> </div>
 
<div align="center"> <img src="https://static.igem.org/mediawiki/2015/8/83/Team_Jilin_China_project3.png" height="170px"> </div>
 
<div align="center"> <img src="https://static.igem.org/mediawiki/2015/8/83/Team_Jilin_China_project3.png" height="170px"> </div>
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<p> First, we adjusted prior vector by adding a gene called T7 RNA polymerase(Figure 2). What’s more, we used pET vectors to express two key enzymes, and constructed such a vector(Figure 3).</p>
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<p> Finally, when T7 polymerase is expressed, Vector pET-DUET can express PADH and FDH. In this way, the whole system can be induced by formaldehyde and express PADH and FDH to degrade it.</p>
 
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Revision as of 21:08, 16 September 2015

describtion

First, we adjusted prior vector by adding a gene called T7 RNA polymerase(Figure 2). What’s more, we used pET vectors to express two key enzymes, and constructed such a vector(Figure 3).

Finally, when T7 polymerase is expressed, Vector pET-DUET can express PADH and FDH. In this way, the whole system can be induced by formaldehyde and express PADH and FDH to degrade it.