Journal of Laboratory

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May 30th, 2015

- Genetic transformation of red coloration [B1010]

- Plasmid removing: [K909008]= tetR-DBD-UVR8

[B1010]= Terminator

[R0040]= Promoter

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June 13th, 2015

- Genetic transformation of [ B1010 ] * failed . We make a new attempt

- Electrophoresis : [ K909008 ]

[ B1010 ]

[ R0040 ]

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June 20th, 2015

- Expanded coloring [ B1010 ]

- Make plasmid removing [ B1010 ]

- Perform digests [ B1010 ] = ( Terminator ) Digestion

[ K909008 ] = ( tetR - DBD - UVR8 ) Double digestion

- electrophoresis : [ B1010 ] 16746p

[ K909008 ] 21506p

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June 27th, 2015

- Purification : [ B0010 ]

- [ K909008 ]

- Plasmid removing : [ E1010 ]

- Electrophoresis : [ B0010 ]

[ K909008 ]

[ E1010 ]

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July 04th, 2015

- Plasmid removing: [J23119]

[B0010]

[K909008]

[R0040]

[E1010]

-Electrophoresis: [B0010]

[R0040]

[K909008]

[J23119]

- Digestion: [B0010]

[K909008]

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July 13th, 2015

- Electrophoresis

- Plasmid Removing

- Terminator digestion and teR-DBD-UVR8

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July 14th, 2015

- Bands Purification:

[K909008]

[B0010]

Join [K909008]

[B0010]

- Electroophoresis: July 04th = [K909008]; [J23119]; [B0010]; [R0040] [K909008]; [R0040]; [B0010]; [R0040]

- Digestion

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July 24th, 2015

- Digestion=

[K909008]= Xbal-PSTI

[J23119]= SPEI-PSTI

[B0010]= EcoRI-Xbal

[R0040]= SPEI-PSTI

[E1010]= Xbal-PSTI

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August 1st, 2015

- Digestion: A[B0010]= E X I

A [R0040]= S P

A [K909008]= X P II

A [J23119]= SPEI PSTI

R [R0040]= S P I

N [R0040]= S P I

N [K909008]= X P II

N [B0010]= E X I

- Genetic Transformation: [J23119]= constitutive promoter

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[K1084103]= RBS

[K909008]= tetR-DBD-UVR8

[B0010]= Terminator

[R0040]= Promoter

[K592009]= purple color

[K592011]= blue color

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August 22nd, 2015

- Digestion: [K1084103]

- Purification: [K909008]

- Genetic transformation: [K592009]

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August 29th, 2015

-Plasmid removing: [K592009]

-Purification: [K909008] Black

[K909008] blue