Difference between revisions of "Team:Wellesley TheTech/Project/Wetlab"
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| − | <img src="https://static.igem.org/mediawiki/2015/1/15/Wellesley_TheTech_Science_wetlab1.png" width=" | + | <img src="https://static.igem.org/mediawiki/2015/1/15/Wellesley_TheTech_Science_wetlab1.png" width="310" height="200" alt="Wet lab prototype 1"> <img src="https://static.igem.org/mediawiki/2015/a/af/Wellesley_TheTech_Science_wetlab2.png" width="310" height="200" alt="Wet lab prototype 2"> <img src="https://static.igem.org/mediawiki/2015/a/a8/Wellesley_TheTech_Science_wetlab3.png" width="310" height="200" alt="Wet lab prototype 3"> |
<p>Description of above image</p> | <p>Description of above image</p> | ||
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<h3>Goal</h3> | <h3>Goal</h3> | ||
Revision as of 23:10, 16 September 2015
WETLAB COMPONENT
Description of above image
Goal
The goal of the wetlab component of BacPack is to connect the digital experience to a hands-on, lab-based one. In the digital activity visitors explore designing bacteria to have a variety of new, useful functions for helping humans survive in different extreme environments. In the wetlab activity visitors get try their hands at the same real-world process of engineering a bacteria to have a new property of their choosing.
Activity Design Outline
Visitors transform a living bacteria to make it do something new:
-5 DNA options that change the physical appearance of bacteria in different ways
-Each ‘color’ represents a bacteria function that can provide one of the 5 needs on Mars
-Visitors pick the DNA they want to use and transform e.coli with it
-They can see a photo of their grown ‘mars bacteria’ several days later
Development
Plasmids:
Selected 5 bioBricks from the iGEM distribution kit:
-Lux Operon (BBa_K325909) - Bioluminescence pathway
amilGFP (BBa_K592010) - Yellow chromoprotein
aeBlue (BBa_K864401) - Blue chromoprotein
eforRed (BBa_K592012) - Red/pink chromoprotein
asPink (BBa_K1033933) - Pink/purple chromoprotein