Difference between revisions of "Team:Northeastern Boston/BasicParts"

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               <h1 id="project_header" style="border-width:0">BASIC & COMPOSITE PARTS</h1>
 
               <h1 id="project_header" style="border-width:0">BASIC & COMPOSITE PARTS</h1>
  
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<h2><a href="http://parts.igem.org/partsdb/edit_seq.cgi?part=BBa_K1857001" target="_blank" style="color:#7CAAD9">BBa_K1857001</a>                     Hygromycin B Resistance Cassette </h2>
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<h2><a href="http://parts.igem.org/partsdb/edit_seq.cgi?part=BBa_K1857001" target="_blank" style="color:#7CAAD9">BBa_K1857001             </a>Hygromycin B Resistance Cassette </h2>
  
 
<img src="https://static.igem.org/mediawiki/2015/0/0c/HygResCassettetake14-01.jpg" style="width:100%">
 
<img src="https://static.igem.org/mediawiki/2015/0/0c/HygResCassettetake14-01.jpg" style="width:100%">

Revision as of 00:56, 17 September 2015

BASIC & COMPOSITE PARTS

BBa_K1857001 Hygromycin B Resistance Cassette

Recognizing that the iGEM repository lacks a modular selection marker for microalgae, Northeastern submits a Hygamycin B resistance cassette. This composite part was cloned from pOpt_mVenus_Hyg and adapted to the iGEM standards. As a complete resistance cassette, this part is expected to confer Hygromycin B resistance to any Chlamydomonas reinhardtii transformed with this part.

This part contains intron 2 of RBCS2 after a fusion HSP70A-RBCS2 promoter. [1] Aph7'', a phosphotransferase, confers resistance to Hygromycin B at agar antibiotic concentrations up to 200g/mL. [2]

mVenus Fluorescent Protein

This part contains mVenus, a nuclear codon-optimized YFP variant (excitation/emission: 515/528nm) [3]. It has Intron 1 and 2 of RBCS2 for increased expression. This is the first independent fluorescent protein codon-optimized for Chlamydomonas reinhardtii submitted to the iGEM registry.