Difference between revisions of "Team:SF Bay Area DIYBio/Safety"

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Team members have received safety training instruction relevant to specific experiments. Both BioCurious and Counter Culture Labs have specific multi-person biosafety teams. One of the team advisors, Patrik D’haeseleer, is on the safety team for both labs and has briefed both teams on the project. Both labs strictly meet the guidelines for BSL-1. <br>
 
Team members have received safety training instruction relevant to specific experiments. Both BioCurious and Counter Culture Labs have specific multi-person biosafety teams. One of the team advisors, Patrik D’haeseleer, is on the safety team for both labs and has briefed both teams on the project. Both labs strictly meet the guidelines for BSL-1. <br>
  
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The risks for members working on this project include the risk of RG1 lab strains. There is no risk to the general public, since we are working with auxotrophic baker’s yeast carrying a plasmid expressing food proteins. To mitigate the risks to the environment from this project we are using yeast that can’t make its own uracil and must be grown on media containing supplement uracil. This yeast strain is highly unlikely to be viable outside of specific lab conditions. Another safety precaution we have put in place to make sure our project stays contained is that only plasmids will be allowed to leave the lab and be transported from one location to the other – no live yeast or purified protein at this stage. As we work on the project we continue to address safety considerations and ensure that the experiments and work is done with best lab practices.</p>
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E. coli (lab strains that are not harmful to humans) will be modified by adding Anabaena pigment genes and antibiotic resistance genes. The organism will also be encouraged to mutate by addition of error prone DNA repair genes. All organisms will be sterilized before disposal. In addition, extra precautions were taken to protect our team from exposure to UV light, including physical barriers surrounding our experiment, as well as safety googles and lab coats. All biological waste is disposed of after bleach sterilization.A safety precaution we have put in place to make sure our project stays contained is that only plasmids will be allowed to leave the lab and be transported from one location to the other – no live bacteria or purified mycosporine at this stage. As we work on the project we continue to address safety considerations and ensure that the experiments and work is done with best lab practices.</p>
  
  
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<p>Please visit <a href="https://2015.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
 
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
 
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<h4>Safe Project Design</h4>
 
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
 
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<li>Choosing a non-pathogenic chassis</li>
 
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<li>Choosing parts that will not harm humans / animals / plants</li>
 
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
 
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<li>Including an "induced lethality" or "kill-switch" device</li>
 
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<h4>Safe Lab Work</h4>
 
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
 
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<h4>Safe Shipment</h4>
 
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
 
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Revision as of 03:30, 19 September 2015

Safety in iGEM

Lab safety considerations for this project
Team members have received safety training instruction relevant to specific experiments. Both BioCurious and Counter Culture Labs have specific multi-person biosafety teams. One of the team advisors, Patrik D’haeseleer, is on the safety team for both labs and has briefed both teams on the project. Both labs strictly meet the guidelines for BSL-1.
E. coli (lab strains that are not harmful to humans) will be modified by adding Anabaena pigment genes and antibiotic resistance genes. The organism will also be encouraged to mutate by addition of error prone DNA repair genes. All organisms will be sterilized before disposal. In addition, extra precautions were taken to protect our team from exposure to UV light, including physical barriers surrounding our experiment, as well as safety googles and lab coats. All biological waste is disposed of after bleach sterilization.A safety precaution we have put in place to make sure our project stays contained is that only plasmids will be allowed to leave the lab and be transported from one location to the other – no live bacteria or purified mycosporine at this stage. As we work on the project we continue to address safety considerations and ensure that the experiments and work is done with best lab practices.