Difference between revisions of "Team:Gifu/Experiment-page"
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<p> In our last study, our long chain protein lost its function. As a reason, we had an idea that the protein lost its holding structure because it was much longer. Therefore, we aimed to synthesize functional long chain proteins by designing some linkers in this year. <br><br> | <p> In our last study, our long chain protein lost its function. As a reason, we had an idea that the protein lost its holding structure because it was much longer. Therefore, we aimed to synthesize functional long chain proteins by designing some linkers in this year. <br><br> | ||
− | <img src="https://static.igem.org/mediawiki/2015/1/18/Gifu-kinou.png"width="605" height="106" align="left" | + | <img src="https://static.igem.org/mediawiki/2015/1/18/Gifu-kinou.png"width="605" height="106" align="left" vspace="25" hspace="50"></img><br> |
<br clear="all"><b>Fig1. the role of linker</b><br></br></p> | <br clear="all"><b>Fig1. the role of linker</b><br></br></p> | ||
<p> | <p> | ||
− | <img src="https://static.igem.org/mediawiki/2015/3/3c/Gifu-kinou2.png"width="346" height="137" align="right" vspace="25" hspace=" | + | <img src="https://static.igem.org/mediawiki/2015/3/3c/Gifu-kinou2.png"width="346" height="137" align="right" vspace="25" hspace="50"></img><br> |
The requirements for amino acids that can be good linkers are less of steric effects and strong in hydropathy. Glycine and serine fulfill this requirements. Glycine’ side chain has almost no steric effects. On the other hand, serine has some steric effects but it has powerful hydropathy because of its hydroxyl side chain. According to Mr. Yabe, because histidine has quite strong hydropathy, though it has big steric effects, it is possible that histidine can work as a linker.<br></p> | The requirements for amino acids that can be good linkers are less of steric effects and strong in hydropathy. Glycine and serine fulfill this requirements. Glycine’ side chain has almost no steric effects. On the other hand, serine has some steric effects but it has powerful hydropathy because of its hydroxyl side chain. According to Mr. Yabe, because histidine has quite strong hydropathy, though it has big steric effects, it is possible that histidine can work as a linker.<br></p> | ||
− | <img src="https://static.igem.org/mediawiki/2015/b/b0/Kinouichiran.png"width="400" height="157" align="right" vspace="25" hspace=" | + | <img src="https://static.igem.org/mediawiki/2015/b/b0/Kinouichiran.png"width="400" height="157" align="right" vspace="25" hspace="50"></img><br> |
Then, we designed some linkers [ see right ]. "GGSGGS", "GSGSGS", and "HHHHHH" were designed as linkers. We combined these sequence and inserted. The pattern of combination was shown right. | Then, we designed some linkers [ see right ]. "GGSGGS", "GSGSGS", and "HHHHHH" were designed as linkers. We combined these sequence and inserted. The pattern of combination was shown right. | ||
<br clear="right"><img src="https://static.igem.org/mediawiki/2015/e/e0/Gifu-kinou3.png"width="432" height="224" align="right" vspace="25" hspace="50"></img><br></br> | <br clear="right"><img src="https://static.igem.org/mediawiki/2015/e/e0/Gifu-kinou3.png"width="432" height="224" align="right" vspace="25" hspace="50"></img><br></br> |
Revision as of 04:03, 17 September 2015