Difference between revisions of "Team:Gifu/result-page"
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</p><br><br> | </p><br><br> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/c/c8/Gifu-pic2.png" width="360" height="173" vspace="10" hspace="50" align="left"></img> | ||
<p> Ct is a value that the more the gene template in PCR increases, the more Ct value decreases. If there is a difference between “C” and “D”, there is a difference in the quantity of template geneThus, the difference between “C” and “D” is small; the cyclization may be efficiency.As a result, Ct in "normal" was 2.31, but “outside”, “inside ①”, “inside ②” were 1.28, 2.02, 1.89 collectively. From these things, it can be said that the efficiency of cyclization rose with all devices which we designed in this time. Especially, in the case of “outside”, there was a difference with Ct more than 1 point than “normal”. When we think that a quantity of the gene doubles for 1 cycle in PCR simply, it can be said that the cyclic efficiency of “outside” was twice as high as that of “normal”. <br> | <p> Ct is a value that the more the gene template in PCR increases, the more Ct value decreases. If there is a difference between “C” and “D”, there is a difference in the quantity of template geneThus, the difference between “C” and “D” is small; the cyclization may be efficiency.As a result, Ct in "normal" was 2.31, but “outside”, “inside ①”, “inside ②” were 1.28, 2.02, 1.89 collectively. From these things, it can be said that the efficiency of cyclization rose with all devices which we designed in this time. Especially, in the case of “outside”, there was a difference with Ct more than 1 point than “normal”. When we think that a quantity of the gene doubles for 1 cycle in PCR simply, it can be said that the cyclic efficiency of “outside” was twice as high as that of “normal”. <br> | ||
After all, it is thought that it fitted this splice site because it is a complementarity chain derived from the creature. | After all, it is thought that it fitted this splice site because it is a complementarity chain derived from the creature. | ||
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We made parts which have these sequence of linker in the downstream of the 3’ side of the intron (Bba_K1332005) or the upstream of the 5’ side of the intron without stop codon (Bba_K1332003).<br> | We made parts which have these sequence of linker in the downstream of the 3’ side of the intron (Bba_K1332005) or the upstream of the 5’ side of the intron without stop codon (Bba_K1332003).<br> | ||
We constructed plasmids like following it.<br></p> | We constructed plasmids like following it.<br></p> | ||
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<p> In case of inserting these plasmid into <i>E. coli</i>, the following circular mRNA is expressed and the long chain protein is synthesized.</p><br> | <p> In case of inserting these plasmid into <i>E. coli</i>, the following circular mRNA is expressed and the long chain protein is synthesized.</p><br> | ||
Revision as of 09:11, 17 September 2015