Difference between revisions of "Team:Gifu/result-page"
Line 89: | Line 89: | ||
</p><br><br> | </p><br><br> | ||
<img src="https://static.igem.org/mediawiki/2015/c/c8/Gifu-pic2.png" width="360" height="173" vspace="10" hspace="50" align="right"></img> | <img src="https://static.igem.org/mediawiki/2015/c/c8/Gifu-pic2.png" width="360" height="173" vspace="10" hspace="50" align="right"></img> | ||
+ | |||
+ | <p> | ||
+ | According to the line e and f, RNA was not detected by the electrophoresis, namely, DNA did not contaminate. | ||
+ | According to the line b and d, linear mRNA was cleaved by exoribonuclease. | ||
+ | According to the line a and c, mRNA was not cleaved by exoribonuclease. | ||
+ | From the above, the circular mRNA existed in all samples. | ||
+ | </p> | ||
+ | |||
+ | |||
<p> Ct is a value that the more the gene template in PCR increases, the more Ct value decreases. If there is a difference between “C” and “D”, there is a difference in the quantity of template geneThus, the difference between “C” and “D” is small; the cyclization may be efficiency.As a result, Ct in "normal" was 2.31, but “outside”, “inside ①”, “inside ②” were 1.28, 2.02, 1.89 collectively. From these things, it can be said that the efficiency of cyclization rose with all devices which we designed in this time. Especially, in the case of “outside”, there was a difference with Ct more than 1 point than “normal”. When we think that a quantity of the gene doubles for 1 cycle in PCR simply, it can be said that the cyclic efficiency of “outside” was twice as high as that of “normal”. <br> | <p> Ct is a value that the more the gene template in PCR increases, the more Ct value decreases. If there is a difference between “C” and “D”, there is a difference in the quantity of template geneThus, the difference between “C” and “D” is small; the cyclization may be efficiency.As a result, Ct in "normal" was 2.31, but “outside”, “inside ①”, “inside ②” were 1.28, 2.02, 1.89 collectively. From these things, it can be said that the efficiency of cyclization rose with all devices which we designed in this time. Especially, in the case of “outside”, there was a difference with Ct more than 1 point than “normal”. When we think that a quantity of the gene doubles for 1 cycle in PCR simply, it can be said that the cyclic efficiency of “outside” was twice as high as that of “normal”. <br> | ||
After all, it is thought that it fitted this splice site because it is a complementarity chain derived from the creature. | After all, it is thought that it fitted this splice site because it is a complementarity chain derived from the creature. |
Revision as of 09:18, 17 September 2015