Difference between revisions of "Team:CGU Taiwan/Notebook"

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<div class="panel panel-default">
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<div class="panel-heading">
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<h4 class="panel-title">
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<a data-toggle="collapse" data-parent="#accordion" href="#collapse1">2015.7.1</a>
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</h4>
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</div>
 +
<div id="collapse1" class="panel-collapse collapse ">
 +
<div class="panel-body">
 +
Operator: Wan yun, Jinting , Jinye<br>
 +
Goal:
 +
<br>
 +
&nbsp;&nbsp;1. Extraction of gDNA of Far1∆::KANMX strain<br>
 +
&nbsp;&nbsp;2. Detection the concentration of fast extracted gDNA of ∆Far1 strain<br>
 +
&nbsp;&nbsp;3. PCR gDNA of Far1∆ ::KANMX<br>
 +
&nbsp;&nbsp;4. Electrophoresis to check PCR product<br>
 +
Experiment steps:<br>
 +
< Extraction of gDNA of Far1∆::KANMX strain><br>
 +
Consult the protocol<protocol of fast extraction of gDNA of yeast><br>
 +
 +
<Detection the concentration fo fast extracted gDNA>
 +
<table class="protocol-table">
 +
<thead>
 +
<tr><th></th><th></th></tr>
 +
</thead>
 +
<tr><td>Strains </td><td>260/280 </td><td>260/230 </td><td>C(ng/μl) </td></tr>
 +
<tr><td>Far1∆::KANMX strain </td><td>1.62 </td><td>0.97 </td><td>44.7 </td></tr>
 +
</table>
 +
<br>
 +
<PCR gDNA of Far1∆::KANMX ><br>
 +
1. Design of primers<br>
 +
<table class="protocol-table">
 +
<thead>
 +
<tr><th></th><th></th></tr>
 +
</thead>
 +
<tr><td> Name</td><td>Pur. </td><td>Seq.(5’-3’) </td><td>Size (mer.) </td><td> MW (g/mol)</td><td>Tm (℃)</td><td>GC (%)</td><td>Nmole</td><td>μl for 100μM </td></tr>
 +
<tr><td>dFAR1 F’</td><td>Desalt </td><td>ggTTTTgTTAggCgggCAAg </td><td>20 </td><td>6244.1 </td><td>53.8 </td><td>55</td><td>21.25</td><td>212.50</td></tr>
 +
<tr><td>dFAR1 R’</td><td>Desalt </td><td>CATTAACTgCTATTTACgACgC </td><td>22 </td><td>6669.4 </td><td>51.1 </td><td>41</td><td>17.12</td><td>171.20</td></tr>
 +
</table>
 +
2. PCR program
 +
 +
 +
 +
<table class="protocol-table">
 +
<thead>
 +
<tr><th></th><th></th><th></th></tr>
 +
</thead>
 +
<tr><td>Step </td><td>Temperature </td><td>Time</td></tr>
 +
<tr><td>Step1 </td><td>95℃ </td><td>5min</td></tr>
 +
<tr><td>Step2 </td><td>95℃ </td><td>30s</td></tr>
 +
<tr><td>Step3 </td><td>52℃ </td><td>30s</td></tr>
 +
<tr><td>Step4→step2 for 30 cycle </td><td>72℃ </td><td>2min </td></tr>
 +
<tr><td>Step5 </td><td>72℃ </td><td>5min </td></tr>
 +
<tr><td>Step6 (hold on) </td><td>10℃ </td><td>1h </td></tr>
 +
 +
</table>
 +
 +
 +
<table class="protocol-table">
 +
<thead>
 +
<tr><th></th><th></th></tr>
 +
</thead>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
</table>
 +
</div>
 +
</div>
 +
</div>
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<div class="panel panel-default">
 
<div class="panel panel-default">
 
<div class="panel-heading">
 
<div class="panel-heading">
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</div>
 
</div>
 
</div>
 
</div>
 +
 +
<div class="panel panel-default">
 +
<div class="panel-heading">
 +
<h4 class="panel-title">
 +
<a data-toggle="collapse" data-parent="#accordion" href="#collapse1">2015.7.1</a>
 +
</h4>
 +
</div>
 +
<div id="collapse1" class="panel-collapse collapse ">
 +
<div class="panel-body">
 +
Operator: Wan yun, Jinting , Jinye
 +
Goal:
 +
1. Extraction of gDNA of Far1∆::KANMX strain
 +
    2. Detection the concentration of fast extracted gDNA of ∆Far1 strain
 +
3. PCR gDNA of Far1∆ ::KANMX
 +
4. Electrophoresis to check PCR product
 +
Experiment steps:
 +
< Extraction of gDNA of Far1∆::KANMX strain>
 +
Consult the protocol<protocol of fast extraction of gDNA of yeast>
 +
 +
<Detection the concentration fo fast extracted gDNA>
 +
<table class="protocol-table">
 +
<thead>
 +
<tr><th></th><th></th></tr>
 +
</thead>
 +
<tr><td>Strains </td><td>260/280 </td><td>260/230 </td><td>C(ng/μl) </td></tr>
 +
<tr><td>Far1∆::KANMX strain </td><td>1.62 </td><td>0.97 </td><td>44.7 </td></tr>
 +
</table>
 +
 +
<PCR gDNA of Far1∆::KANMX >
 +
1. Design of primers
 +
<table class="protocol-table">
 +
<thead>
 +
<tr><th></th><th></th></tr>
 +
</thead>
 +
<tr><td> </td><td> </td><td> </td><td> </td><td> </td><td>Tm (℃)</td><td>GC (%)</td><td>Nmole</td><td>μl for 100μM </td></tr>
 +
<tr><td> </td><td> </td><td> </td><td> </td><td> </td><td> </td><td>55</td><td>21.25</td><td>212.50</td></tr>
 +
<tr><td> </td><td> </td><td> </td><td> </td><td> </td><td> </td><td>41</td><td>17.12</td><td>171.20</td></tr>
 +
</table>
 +
 +
 +
 +
 +
<table class="protocol-table">
 +
<thead>
 +
<tr><th></th><th></th></tr>
 +
</thead>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
</table>
 +
 +
 +
<table class="protocol-table">
 +
<thead>
 +
<tr><th></th><th></th></tr>
 +
</thead>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
</table>
 +
</div>
 +
</div>
 +
</div>
 +
 +
<div class="panel panel-default">
 +
<div class="panel-heading">
 +
<h4 class="panel-title">
 +
<a data-toggle="collapse" data-parent="#accordion" href="#collapse1">2015.7.1</a>
 +
</h4>
 +
</div>
 +
<div id="collapse1" class="panel-collapse collapse ">
 +
<div class="panel-body">
 +
Operator: Wan yun, Jinting , Jinye
 +
Goal:
 +
1. Extraction of gDNA of Far1∆::KANMX strain
 +
    2. Detection the concentration of fast extracted gDNA of ∆Far1 strain
 +
3. PCR gDNA of Far1∆ ::KANMX
 +
4. Electrophoresis to check PCR product
 +
Experiment steps:
 +
< Extraction of gDNA of Far1∆::KANMX strain>
 +
Consult the protocol<protocol of fast extraction of gDNA of yeast>
 +
 +
<Detection the concentration fo fast extracted gDNA>
 +
<table class="protocol-table">
 +
<thead>
 +
<tr><th></th><th></th></tr>
 +
</thead>
 +
<tr><td>Strains </td><td>260/280 </td><td>260/230 </td><td>C(ng/μl) </td></tr>
 +
<tr><td>Far1∆::KANMX strain </td><td>1.62 </td><td>0.97 </td><td>44.7 </td></tr>
 +
</table>
 +
 +
<PCR gDNA of Far1∆::KANMX >
 +
1. Design of primers
 +
<table class="protocol-table">
 +
<thead>
 +
<tr><th></th><th></th></tr>
 +
</thead>
 +
<tr><td> </td><td> </td><td> </td><td> </td><td> </td><td>Tm (℃)</td><td>GC (%)</td><td>Nmole</td><td>μl for 100μM </td></tr>
 +
<tr><td> </td><td> </td><td> </td><td> </td><td> </td><td> </td><td>55</td><td>21.25</td><td>212.50</td></tr>
 +
<tr><td> </td><td> </td><td> </td><td> </td><td> </td><td> </td><td>41</td><td>17.12</td><td>171.20</td></tr>
 +
</table>
 +
 +
 +
 +
 +
<table class="protocol-table">
 +
<thead>
 +
<tr><th></th><th></th></tr>
 +
</thead>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
</table>
 +
 +
 +
<table class="protocol-table">
 +
<thead>
 +
<tr><th></th><th></th></tr>
 +
</thead>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
</table>
 +
</div>
 +
</div>
 +
</div>
 +
 +
<div class="panel panel-default">
 +
<div class="panel-heading">
 +
<h4 class="panel-title">
 +
<a data-toggle="collapse" data-parent="#accordion" href="#collapse1">2015.7.1</a>
 +
</h4>
 +
</div>
 +
<div id="collapse1" class="panel-collapse collapse ">
 +
<div class="panel-body">
 +
Operator: Wan yun, Jinting , Jinye
 +
Goal:
 +
1. Extraction of gDNA of Far1∆::KANMX strain
 +
    2. Detection the concentration of fast extracted gDNA of ∆Far1 strain
 +
3. PCR gDNA of Far1∆ ::KANMX
 +
4. Electrophoresis to check PCR product
 +
Experiment steps:
 +
< Extraction of gDNA of Far1∆::KANMX strain>
 +
Consult the protocol<protocol of fast extraction of gDNA of yeast>
 +
 +
<Detection the concentration fo fast extracted gDNA>
 +
<table class="protocol-table">
 +
<thead>
 +
<tr><th></th><th></th></tr>
 +
</thead>
 +
<tr><td>Strains </td><td>260/280 </td><td>260/230 </td><td>C(ng/μl) </td></tr>
 +
<tr><td>Far1∆::KANMX strain </td><td>1.62 </td><td>0.97 </td><td>44.7 </td></tr>
 +
</table>
 +
 +
<PCR gDNA of Far1∆::KANMX >
 +
1. Design of primers
 +
<table class="protocol-table">
 +
<thead>
 +
<tr><th></th><th></th></tr>
 +
</thead>
 +
<tr><td> </td><td> </td><td> </td><td> </td><td> </td><td>Tm (℃)</td><td>GC (%)</td><td>Nmole</td><td>μl for 100μM </td></tr>
 +
<tr><td> </td><td> </td><td> </td><td> </td><td> </td><td> </td><td>55</td><td>21.25</td><td>212.50</td></tr>
 +
<tr><td> </td><td> </td><td> </td><td> </td><td> </td><td> </td><td>41</td><td>17.12</td><td>171.20</td></tr>
 +
</table>
 +
 +
 +
 +
 +
<table class="protocol-table">
 +
<thead>
 +
<tr><th></th><th></th></tr>
 +
</thead>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
</table>
 +
 +
 +
<table class="protocol-table">
 +
<thead>
 +
<tr><th></th><th></th></tr>
 +
</thead>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
</table>
 +
</div>
 +
</div>
 +
</div>
 +
 +
<div class="panel panel-default">
 +
<div class="panel-heading">
 +
<h4 class="panel-title">
 +
<a data-toggle="collapse" data-parent="#accordion" href="#collapse1">2015.7.1</a>
 +
</h4>
 +
</div>
 +
<div id="collapse1" class="panel-collapse collapse ">
 +
<div class="panel-body">
 +
Operator: Wan yun, Jinting , Jinye
 +
Goal:
 +
1. Extraction of gDNA of Far1∆::KANMX strain
 +
    2. Detection the concentration of fast extracted gDNA of ∆Far1 strain
 +
3. PCR gDNA of Far1∆ ::KANMX
 +
4. Electrophoresis to check PCR product
 +
Experiment steps:
 +
< Extraction of gDNA of Far1∆::KANMX strain>
 +
Consult the protocol<protocol of fast extraction of gDNA of yeast>
 +
 +
<Detection the concentration fo fast extracted gDNA>
 +
<table class="protocol-table">
 +
<thead>
 +
<tr><th></th><th></th></tr>
 +
</thead>
 +
<tr><td>Strains </td><td>260/280 </td><td>260/230 </td><td>C(ng/μl) </td></tr>
 +
<tr><td>Far1∆::KANMX strain </td><td>1.62 </td><td>0.97 </td><td>44.7 </td></tr>
 +
</table>
 +
 +
<PCR gDNA of Far1∆::KANMX >
 +
1. Design of primers
 +
<table class="protocol-table">
 +
<thead>
 +
<tr><th></th><th></th></tr>
 +
</thead>
 +
<tr><td> </td><td> </td><td> </td><td> </td><td> </td><td>Tm (℃)</td><td>GC (%)</td><td>Nmole</td><td>μl for 100μM </td></tr>
 +
<tr><td> </td><td> </td><td> </td><td> </td><td> </td><td> </td><td>55</td><td>21.25</td><td>212.50</td></tr>
 +
<tr><td> </td><td> </td><td> </td><td> </td><td> </td><td> </td><td>41</td><td>17.12</td><td>171.20</td></tr>
 +
</table>
 +
 +
 +
 +
 +
<table class="protocol-table">
 +
<thead>
 +
<tr><th></th><th></th></tr>
 +
</thead>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
</table>
 +
 +
 +
<table class="protocol-table">
 +
<thead>
 +
<tr><th></th><th></th></tr>
 +
</thead>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
</table>
 +
</div>
 +
</div>
 +
</div>
 +
 +
<div class="panel panel-default">
 +
<div class="panel-heading">
 +
<h4 class="panel-title">
 +
<a data-toggle="collapse" data-parent="#accordion" href="#collapse1">2015.7.1</a>
 +
</h4>
 +
</div>
 +
<div id="collapse1" class="panel-collapse collapse ">
 +
<div class="panel-body">
 +
Operator: Wan yun, Jinting , Jinye
 +
Goal:
 +
1. Extraction of gDNA of Far1∆::KANMX strain
 +
    2. Detection the concentration of fast extracted gDNA of ∆Far1 strain
 +
3. PCR gDNA of Far1∆ ::KANMX
 +
4. Electrophoresis to check PCR product
 +
Experiment steps:
 +
< Extraction of gDNA of Far1∆::KANMX strain>
 +
Consult the protocol<protocol of fast extraction of gDNA of yeast>
 +
 +
<Detection the concentration fo fast extracted gDNA>
 +
<table class="protocol-table">
 +
<thead>
 +
<tr><th></th><th></th></tr>
 +
</thead>
 +
<tr><td>Strains </td><td>260/280 </td><td>260/230 </td><td>C(ng/μl) </td></tr>
 +
<tr><td>Far1∆::KANMX strain </td><td>1.62 </td><td>0.97 </td><td>44.7 </td></tr>
 +
</table>
 +
 +
<PCR gDNA of Far1∆::KANMX >
 +
1. Design of primers
 +
<table class="protocol-table">
 +
<thead>
 +
<tr><th></th><th></th></tr>
 +
</thead>
 +
<tr><td> </td><td> </td><td> </td><td> </td><td> </td><td>Tm (℃)</td><td>GC (%)</td><td>Nmole</td><td>μl for 100μM </td></tr>
 +
<tr><td> </td><td> </td><td> </td><td> </td><td> </td><td> </td><td>55</td><td>21.25</td><td>212.50</td></tr>
 +
<tr><td> </td><td> </td><td> </td><td> </td><td> </td><td> </td><td>41</td><td>17.12</td><td>171.20</td></tr>
 +
</table>
 +
 +
 +
 +
 +
<table class="protocol-table">
 +
<thead>
 +
<tr><th></th><th></th></tr>
 +
</thead>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
</table>
 +
 +
 +
<table class="protocol-table">
 +
<thead>
 +
<tr><th></th><th></th></tr>
 +
</thead>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
</table>
 +
</div>
 +
</div>
 +
</div>
 +
 +
<div class="panel panel-default">
 +
<div class="panel-heading">
 +
<h4 class="panel-title">
 +
<a data-toggle="collapse" data-parent="#accordion" href="#collapse1">2015.7.1</a>
 +
</h4>
 +
</div>
 +
<div id="collapse1" class="panel-collapse collapse ">
 +
<div class="panel-body">
 +
Operator: Wan yun, Jinting , Jinye
 +
Goal:
 +
1. Extraction of gDNA of Far1∆::KANMX strain
 +
    2. Detection the concentration of fast extracted gDNA of ∆Far1 strain
 +
3. PCR gDNA of Far1∆ ::KANMX
 +
4. Electrophoresis to check PCR product
 +
Experiment steps:
 +
< Extraction of gDNA of Far1∆::KANMX strain>
 +
Consult the protocol<protocol of fast extraction of gDNA of yeast>
 +
 +
<Detection the concentration fo fast extracted gDNA>
 +
<table class="protocol-table">
 +
<thead>
 +
<tr><th></th><th></th></tr>
 +
</thead>
 +
<tr><td>Strains </td><td>260/280 </td><td>260/230 </td><td>C(ng/μl) </td></tr>
 +
<tr><td>Far1∆::KANMX strain </td><td>1.62 </td><td>0.97 </td><td>44.7 </td></tr>
 +
</table>
 +
 +
<PCR gDNA of Far1∆::KANMX >
 +
1. Design of primers
 +
<table class="protocol-table">
 +
<thead>
 +
<tr><th></th><th></th></tr>
 +
</thead>
 +
<tr><td> </td><td> </td><td> </td><td> </td><td> </td><td>Tm (℃)</td><td>GC (%)</td><td>Nmole</td><td>μl for 100μM </td></tr>
 +
<tr><td> </td><td> </td><td> </td><td> </td><td> </td><td> </td><td>55</td><td>21.25</td><td>212.50</td></tr>
 +
<tr><td> </td><td> </td><td> </td><td> </td><td> </td><td> </td><td>41</td><td>17.12</td><td>171.20</td></tr>
 +
</table>
 +
 +
 +
 +
 +
<table class="protocol-table">
 +
<thead>
 +
<tr><th></th><th></th></tr>
 +
</thead>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
<tr><td> </td><td> </td></tr>
 +
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Revision as of 12:35, 17 September 2015

Home | CGU_Taiwan

Home | CGU_Taiwan

DescriptionYeast With IL-8 ReceptorToehold Switch As RNA SenorPrototype Design

GPCR

2015.7.1

Operator: Wan yun, Jinting , Jinye
Goal:
  1. Extraction of gDNA of Far1∆::KANMX strain
  2. Detection the concentration of fast extracted gDNA of ∆Far1 strain
  3. PCR gDNA of Far1∆ ::KANMX
  4. Electrophoresis to check PCR product
Experiment steps:
< Extraction of gDNA of Far1∆::KANMX strain>
Consult the protocol
Strains 260/280 260/230 C(ng/μl)
Far1∆::KANMX strain 1.62 0.97 44.7


1. Design of primers
NamePur. Seq.(5’-3’) Size (mer.) MW (g/mol)Tm (℃)GC (%)Nmoleμl for 100μM
dFAR1 F’Desalt ggTTTTgTTAggCgggCAAg 20 6244.1 53.8 5521.25212.50
dFAR1 R’Desalt CATTAACTgCTATTTACgACgC 22 6669.4 51.1 4117.12171.20
2. PCR program
Step Temperature Time
Step1 95℃ 5min
Step2 95℃ 30s
Step3 52℃ 30s
Step4→step2 for 30 cycle 72℃ 2min
Step5 72℃ 5min
Step6 (hold on) 10℃ 1h

2015.7.1

Operator: Wan yun, Jinting , Jinye Goal: 1. Extraction of gDNA of Far1∆::KANMX strain 2. Detection the concentration of fast extracted gDNA of ∆Far1 strain 3. PCR gDNA of Far1∆ ::KANMX 4. Electrophoresis to check PCR product Experiment steps: < Extraction of gDNA of Far1∆::KANMX strain> Consult the protocol
Strains 260/280 260/230 C(ng/μl)
Far1∆::KANMX strain 1.62 0.97 44.7
1. Design of primers
Tm (℃)GC (%)Nmoleμl for 100μM
5521.25212.50
4117.12171.20

2015.7.1

Operator: Wan yun, Jinting , Jinye Goal: 1. Extraction of gDNA of Far1∆::KANMX strain 2. Detection the concentration of fast extracted gDNA of ∆Far1 strain 3. PCR gDNA of Far1∆ ::KANMX 4. Electrophoresis to check PCR product Experiment steps: < Extraction of gDNA of Far1∆::KANMX strain> Consult the protocol
Strains 260/280 260/230 C(ng/μl)
Far1∆::KANMX strain 1.62 0.97 44.7
1. Design of primers
Tm (℃)GC (%)Nmoleμl for 100μM
5521.25212.50
4117.12171.20

2015.7.1

Operator: Wan yun, Jinting , Jinye Goal: 1. Extraction of gDNA of Far1∆::KANMX strain 2. Detection the concentration of fast extracted gDNA of ∆Far1 strain 3. PCR gDNA of Far1∆ ::KANMX 4. Electrophoresis to check PCR product Experiment steps: < Extraction of gDNA of Far1∆::KANMX strain> Consult the protocol
Strains 260/280 260/230 C(ng/μl)
Far1∆::KANMX strain 1.62 0.97 44.7
1. Design of primers
Tm (℃)GC (%)Nmoleμl for 100μM
5521.25212.50
4117.12171.20

2015.7.1

Operator: Wan yun, Jinting , Jinye Goal: 1. Extraction of gDNA of Far1∆::KANMX strain 2. Detection the concentration of fast extracted gDNA of ∆Far1 strain 3. PCR gDNA of Far1∆ ::KANMX 4. Electrophoresis to check PCR product Experiment steps: < Extraction of gDNA of Far1∆::KANMX strain> Consult the protocol
Strains 260/280 260/230 C(ng/μl)
Far1∆::KANMX strain 1.62 0.97 44.7
1. Design of primers
Tm (℃)GC (%)Nmoleμl for 100μM
5521.25212.50
4117.12171.20

2015.7.1

Operator: Wan yun, Jinting , Jinye Goal: 1. Extraction of gDNA of Far1∆::KANMX strain 2. Detection the concentration of fast extracted gDNA of ∆Far1 strain 3. PCR gDNA of Far1∆ ::KANMX 4. Electrophoresis to check PCR product Experiment steps: < Extraction of gDNA of Far1∆::KANMX strain> Consult the protocol
Strains 260/280 260/230 C(ng/μl)
Far1∆::KANMX strain 1.62 0.97 44.7
1. Design of primers
Tm (℃)GC (%)Nmoleμl for 100μM
5521.25212.50
4117.12171.20

2015.7.1

Operator: Wan yun, Jinting , Jinye Goal: 1. Extraction of gDNA of Far1∆::KANMX strain 2. Detection the concentration of fast extracted gDNA of ∆Far1 strain 3. PCR gDNA of Far1∆ ::KANMX 4. Electrophoresis to check PCR product Experiment steps: < Extraction of gDNA of Far1∆::KANMX strain> Consult the protocol
Strains 260/280 260/230 C(ng/μl)
Far1∆::KANMX strain 1.62 0.97 44.7
1. Design of primers
Tm (℃)GC (%)Nmoleμl for 100μM
5521.25212.50
4117.12171.20

2015.7.1

Operator: Wan yun, Jinting , Jinye Goal: 1. Extraction of gDNA of Far1∆::KANMX strain 2. Detection the concentration of fast extracted gDNA of ∆Far1 strain 3. PCR gDNA of Far1∆ ::KANMX 4. Electrophoresis to check PCR product Experiment steps: < Extraction of gDNA of Far1∆::KANMX strain> Consult the protocol
Strains 260/280 260/230 C(ng/μl)
Far1∆::KANMX strain 1.62 0.97 44.7
1. Design of primers
Tm (℃)GC (%)Nmoleμl for 100μM
5521.25212.50
4117.12171.20

RNA

2015.7.1

Operator: Wan yun, Jinting , Jinye Goal: 1. Extraction of gDNA of Far1∆::KANMX strain 2. Detection the concentration of fast extracted gDNA of ∆Far1 strain 3. PCR gDNA of Far1∆ ::KANMX 4. Electrophoresis to check PCR product Experiment steps: < Extraction of gDNA of Far1∆::KANMX strain> Consult the protocol
Strains 260/280 260/230 C(ng/μl)
Far1∆::KANMX strain 1.62 0.97 44.7
1. Design of primers
Tm (℃)GC (%)Nmoleμl for 100μM
5521.25212.50
4117.12171.20