Difference between revisions of "Team:CHINA CD UESTC/Design"
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− | <p>The laccase can be used to construct a common EBFC as biological cathode. we conceived a prototype(figure 3). In the EBFC, we used carbon paper which was full of carbon microfibers as electrode because it has good conductivity and large surface area. Glucose oxidase and RFP+laccase were chosed to catalyze reaction for producing electricity! | + | <p>The laccase can be used to construct a common EBFC as biological cathode. we conceived a prototype( figure 3). In the EBFC, we used carbon paper which was full of carbon microfibers as electrode because it has good conductivity and large surface area. Glucose oxidase and RFP+laccase were chosed to catalyze reaction for producing electricity! |
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− | <h4>3. | + | <h4>3. Connection magnetosome with laccase</h4> |
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− | + | Meanwhile, in order to make laccase enriched, we designed a recombinant vector to fuse express <i>mamW</i> and <i>RFP</i> with the laccase. The protein mamW, a magnetosome transmembrane protein, can connect laccase and magnetosome. The RFP reporter can make the novel structure visible. So we designed the vector piGEM-WRL. As the vector will be co-transferred with another two vectors, we chose the pACYCDuet-1 as the backbone. | |
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− | <img src="https://static.igem.org/mediawiki/2015/ | + | <img src="https://static.igem.org/mediawiki/2015/9/90/CHINA_CD_UESTC_DESIGN_LACCASE01.png" width="50%"> |
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− | <strong>Figure | + | <strong>Figure 3.</strong> Schematic of piGEM-WRL construction. The protein MamW is a magnetosome transmembrane protein <sup>[2]</sup>. MamW gene was amplified from the <strong><i>Ms.gryphiswaldense MSR-1</i></strong> |
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Revision as of 12:14, 17 September 2015
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DESIGN
We mainly designed three vectors respectively carrying mamW + RFP +laccase , mamAB and mamGFDC + mms6 + mamXY . Our purpose is to accomplish our magnetotactic E.coli and fix the laccase on the magnetosome membrane. Finally we put the magnetosomes carrying laccases into our enzymatic bio-fuel cell (EBFC).