Difference between revisions of "Team:UST Beijing"

Revision as of 12:51, 17 September 2015

We know that E.coli divides in LB medium at 37C around 20-30min per cycle. In this 20-30 minutes, the bacterium has to duplicate its DNA, 4.5 million base pairs, produce every protein of its body another copy, assemble them into structures, and divide into two almost identical copies of individual bacterium.

On top of this, if we introduce a plasmid of 3kb long, 500 copies of it, into every E.coli individual, we would add some pressure to this replicating process. In this plasmid, a RFP protein expression cassette (part number J022150?) is inserted. The promoter has a lacI operon but in DH5alpha strain, its basal expression seems not under tight LacI control, probably due to weak expression levels of LacI in DH5alpha, and its huge copy numbers.

Once upon a time, there was a group of lovely red fluorescent protein plasmid. One day they met another very lovely group of e. coli, and they play together happily. China has an old saying: He who gets in contact with vermilion will become red. The e. coli who Gets along well with red fluorescent protein plasmid were dyed red. But some red fluorescent protein plasmid and some e. coli are shy, this part of e. coli is not dyed red. What happened between them? Let's explore their story~

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Difference between revisions of "Team:UST Beijing"

Revision as of 12:51, 17 September 2015

Individual Variation of RFP accumulation in E.coli

AGE-RELATED PHENOMENA: A HYPOTHESIS

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