Difference between revisions of "Team:TU Darmstadt/Notebook/sec1/K1602025"
| Line 4: | Line 4: | ||
<html><div class="contentSection"> | <html><div class="contentSection"> | ||
| + | <p align="justify"> | ||
<a href=" https://2015.igem.org/Team:TU_Darmstadt/Notebook/sec1/K1602024 " title="Opens internal link in current window" class="internal link">K1602024</a> | <a href=" https://2015.igem.org/Team:TU_Darmstadt/Notebook/sec1/K1602024 " title="Opens internal link in current window" class="internal link">K1602024</a> | ||
was digested with <em>Xba</em>I and <em>Pst</em>I and ligated into B0034-pSB1A2. Competent <em>E. coli</em> Top 10 were transformed via heat shock. Colonies were screened by colony PCR with oligonucleotides VF2 and VR. Plasmids of positive clones were extracted. The construct was digested with <em>EcoR</em>I and <em>Pst</em>I and ligated into pSB1C3. Competent <em>E. coli</em> Top 10 were transformed via heat shock. | was digested with <em>Xba</em>I and <em>Pst</em>I and ligated into B0034-pSB1A2. Competent <em>E. coli</em> Top 10 were transformed via heat shock. Colonies were screened by colony PCR with oligonucleotides VF2 and VR. Plasmids of positive clones were extracted. The construct was digested with <em>EcoR</em>I and <em>Pst</em>I and ligated into pSB1C3. Competent <em>E. coli</em> Top 10 were transformed via heat shock. | ||
Colonies were screened by colony PCR with oligonucleotides VF2 and VR. | Colonies were screened by colony PCR with oligonucleotides VF2 and VR. | ||
| + | </p> | ||
</div></html> | </div></html> | ||
Revision as of 17:42, 17 September 2015
K1602025 - B0034-HistidinTag-Green fluorescent protein (B0034-His-GFP)
K1602024 was digested with XbaI and PstI and ligated into B0034-pSB1A2. Competent E. coli Top 10 were transformed via heat shock. Colonies were screened by colony PCR with oligonucleotides VF2 and VR. Plasmids of positive clones were extracted. The construct was digested with EcoRI and PstI and ligated into pSB1C3. Competent E. coli Top 10 were transformed via heat shock. Colonies were screened by colony PCR with oligonucleotides VF2 and VR.