Difference between revisions of "Team:Cambridge-JIC/Make Your Own js"
| Line 174: | Line 174: | ||
} | } | ||
if(options[3] == 0) { | if(options[3] == 0) { | ||
| − | ret += '<tr><td>1</td><td>amber LED* (RFP)</td><td>1.088</td><td><a href="http://uk.rs-online.com/web/p/visible-leds/8107834/" style="color:#0000bb">Brand: OSRAM Opto Semiconductors; Part No. LY CKBP-JYKX-36-1</a> (must be bought in multiples of 5)</td></tr><tr><td>1</td><td>dichroic mirror* (RFP)</td><td>19.40</td><a href="http://www.comaroptics.com/components/filters/dichroic-filters/long-pass-dichroic-filters" style="color:#0000bb">Mfr: Comar; Part No. 650 IY 116</a | + | ret += '<tr><td>1</td><td>amber LED* (RFP)</td><td>1.088</td><td><a href="http://uk.rs-online.com/web/p/visible-leds/8107834/" style="color:#0000bb">Brand: OSRAM Opto Semiconductors; Part No. LY CKBP-JYKX-36-1</a> (must be bought in multiples of 5)</td></tr><tr><td>1</td><td>dichroic mirror* (RFP)</td><td>19.40</td><td><a href="http://www.comaroptics.com/components/filters/dichroic-filters/long-pass-dichroic-filters" style="color:#0000bb">Mfr: Comar; Part No. 650 IY 116</a></td></tr><tr><td>1</td><td>excitation filter* (RFP)</td><td>19.40</td><td><a href="http://www.comaroptics.com/components/filters/dichroic-filters/long-pass-dichroic-filters" style="color:#0000bb">Mfr: Comar; Part No. 534 IB 116</a></td></tr><tr><td>1</td><td>emission filter* (RFP)</td><td>19.40</td><td><a href="http://www.comaroptics.com/components/filters/gelatin-and-polyester-colour-filters#row-575_wb_75" style="color:#0000bb">Mfr: Comar; Part No. 575 WB 75</a></td></tr>' |
} | } | ||
if(options[4] == 0) { | if(options[4] == 0) { | ||
Revision as of 14:19, 17 September 2015
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q = new quiz('#cam-quiz');
step0 = new q.step("Tell us which options you'd like for your microscope.", {
1: "Okay!"
});
step1 = new q.step("Besides bright-field, do you need dark-field imaging?", {
0: "Yes",
1: "No"
});
step2 = new q.step("Do you need to image GFP fluorescence?", {
0: "Yes",
1: "No"
});
step3 = new q.step("Do you need to image RFP fluorescence?", {
0: "Yes",
1: "No"
});
step4 = new q.step("Do you need to image other fluorescent proteins, e.g. YFP?", {
0: "Yes",
1: "No"
});
step5 = new q.step("Do you want your microscope to be manual, motorised, or will you need to swap between both?", {
0: "Manual only",
1: "Motorised only",
2: "Both",
});
step6 = new q.step("Do you need your microscope to be fully battery-powered, e.g. for fieldwork?", {
0: "Yes",
1: "No"
});
final = function(options) {
window.location.href="#CC0";
ret = '<h3>Thanks!</h3>
Here\'s what you\'ll need\:
<thead>3D PRINTED COMPONENTS # Part Cost/unit (GBP) STL & SCAD files 1 main stage 1 base 2 side supports for z axis 1 z axis in 3 parts (vertical + horizontal + connector) 1 connecting ring bw z axes 1 3-legged camera holder 2 slide clips 1 bright-field cube if(options[1] == 0) {
ret += '1 dark-field tube in 3 parts }
if(options[2] == 0 && options[3] == 0 && options[4] == 0) {
ret += '3 epicubes 3 sets of drawers (for filters and mirrors) }
else if((options[2] == 0 && options[3] == 0) || (options[2] == 0 && options[4] == 0) || (options[3] == 0 && options[4] == 0)) {
ret += '2 epicubes 2 sets of drawers (for filters and mirrors) } else if(options[2] == 0 || options[3] == 0 || options[4] == 0) {
ret += '1 epicube 1 set of drawers (for filters and mirrors) }
if(options[5] == 0 || options[5] == 2) {
ret += '3 screw gears 3 manual gears 3 screw holders }
if(options[5] == 1 || options[5] == 2) {
ret += '3 screw gears for motors 3 motor holders }
if((options[5] == 1 || options[5] == 2) && options[6] == 1) {
ret += '1 battery holder for RPi }
if((options[5] == 1 || options[5] == 2) && options[6] == 0) {
ret += '2 battery holders for RPi }
if(options[6] == 0 && !(options[5] == 1 || options[5] == 2)) {
ret += '1 battery holder for RPi }
ret += '</tbody>
'
ret += '
<thead>OFF-THE-SHELF COMPONENTS # Part Cost/unit (GBP) Details & link to provider 1 white LED (bright-field) 0.554 <a href="http://uk.rs-online.com/web/p/visible-leds/7133955/?searchTerm=NSPW500DS&relevancy-data=636F3D3226696E3D4931384E4B6E6F776E41734D504E266C753D656E266D6D3D6D61746368616C6C7061727469616C26706D3D5E5B5C707B4C7D5C707B4E647D2D2C2F255C2E5D2B2426706F3D313326736E3D592673743D4D414E5F504152545F4E554D4245522677633D424F5448267573743D4E535057353030445326" style="color:#0000bb">Mfr: Nichia; Part No. NSPW500DS</a> (must be bought in multiples of 5) 1 ball lens 12.20 <a href="http://www.comaroptics.com/components/lenses/simple-convex-lenses/sapphire-and-glass-ball-lenses#row-03_vq_04" style="color:#0000bb">Mfr: Comar; Part No: 03 VQ 04</a> 2 25 mm long M4 screws 0.107 Buy at hardware store<a ref="https://www.mackay.co.uk/forgefix-machine-screw-pozi-pan-head-zp-m4-25mm-blister-10-msph425b-screws-fixings-hardware-security-toolbank-brands.html" style="color:#0000bb"> (reference price/unit for a pack of 10)</a> 1 40 mm long M4 screw 0.107 Buy at hardware store<a ref="https://www.mackay.co.uk/forgefix-machine-screw-pozi-pan-head-zp-m4-40mm-blister-6-msph440b-screws-fixings-hardware-security-toolbank-brands.html" style="color:#0000bb"> (reference price/unit for a pack of 10)</a> 3 M4 nuts 0.0043 Buy at hardware store<a ref="https://www.mackay.co.uk/forgefix-hexagon-nut-zp-m4-bag-100-100nut4-hexagonal-nuts-bolts-fixings-hardware-security-toolbank-brands.html" style="color:#0000bb"> (reference price/unit for a pack of 100)</a> 3 M4 washers 0.0037 Buy at hardware store<a ref="https://www.mackay.co.uk/forgefix-flat-washer-heavyduty-zp-m4-bag-100-100hdwash4-washers-bolts-nuts-fixings-hardware-security-toolbank-brands.html" style="color:#0000bb"> (reference price/unit for a pack of 100)</a> 1 Raspberry Pi 2 Model B 26.60 <a href="http://uk.rs-online.com/web/p/processor-microcontroller-development-kits/832-6274/" style="color:#0000bb">Processor part number: BCM2836</a> 1 Ralink RT5370 WiFi adapter 9.10 <a href="http://www.amazon.co.uk/gp/product/B00IQFGJSS?psc=1&redirect=true&ref_=oh_aui_detailpage_o02_s00" style="color:#0000bb">Amazon Standard Identification Number (ASIN)\: B00IQFGJSS</a> 1 Arduino UNO with its USB B connector 21.00 <a href="https://shop.pimoroni.com/products/arduino-uno" style="color:#0000bb">Mfr: Arduino; Part No. A000066</a> 1 breadboard 2.82 <a href="http://uk.farnell.com/multicomp/mcbb400/breadboard-solderless-300v-abs/dp/2395961?ost=2395961&mckv=ezr2Yisy&CMP=KNC-GUK-FUK-GEN-SHOPPING-MULTICOMP&CAGPSPN=pla&gclid=Cj0KEQjwg9-vBRCK7L7wmO2u0JcBEiQA_tzoaEPR46s_SrENpnwFOv5PS_hqUNVzk8VV5QPxB9AZBwYaAupo8P8HAQ&CAWELAID=120173390000249291&gross_price=true" style="color:#0000bb">Mfr: Multicomp; Part No. MCBB400</a> 1 16GB micro SD card 5.43 <a href="http://www.amazon.co.uk/SanDisk-Ultra-Imaging-C10-MicroSDHC/dp/B00MBTP8Q4/ref=sr_1_6?ie=UTF8&qid=1442328635&sr=8-6&keywords=micro+sd+16gb+memory+cards" style="color:#0000bb">Model number: SDSDQUIN-016G-G4; ASIN: B00MBTP8Q4</a> 2 220 ohm resistors 0.065 <a href="http://uk.rs-online.com/web/p/through-hole-fixed-resistors/0131794/" style="color:#0000bb">Brand: TE Connectivity; Part No. CFR100J220R</a> (must be bought in multiples of 10) 1 ethernet cable 9.99 <a href="http://www.pcworld.co.uk/gbuk/computing-accessories/computer-accessories/power-and-cables/computing-cables-and-adaptors/sandstrom-scat65m12-rj45-to-rj45-cat-6-ethernet-cable-5m-12567142-pdt.html" style="color:#0000bb">Mfr: Sandstrøm; Product code: 874926</a> 1 100 m spool insulated single-core wire 13.45 <a href="http://uk.rs-online.com/web/p/single-core-control-cable/7244311/" style="color:#0000bb">Brand: Lapp; Part No. 4510021</a> if(options[2] == 0 && options[3] == 0 && options[4] == 0) {
ret += '3 RPi cameras 22.18 <a href="http://www.misco.co.uk/product/217272/Raspberry-Pi-NoIR-Camera-Board?fromProductList=Search+result" style="color:#0000bb">Brand: Raspberry Pi; Part No. PiNoir</a> 1 RPi ribbon cable 0.00 Comes with RPi camera }
else if((options[2] == 0 && options[3] == 0) || (options[2] == 0 && options[4] == 0) || (options[3] == 0 && options[4] == 0)) {
ret += '2 RPi cameras 22.18 <a href="http://www.misco.co.uk/product/217272/Raspberry-Pi-NoIR-Camera-Board?fromProductList=Search+result" style="color:#0000bb">Brand: Raspberry Pi; Part No. PiNoir</a> 1 RPi ribbon cable 0.00 Comes with RPi camera } else if(options[2] == 0 || options[3] == 0 || options[4] == 0) {
ret += '1 RPi camera 22.18 <a href="http://www.misco.co.uk/product/217272/Raspberry-Pi-NoIR-Camera-Board?fromProductList=Search+result" style="color:#0000bb">Brand: Raspberry Pi; Part No. PiNoir</a> 1 RPi ribbon cable 0.00 Comes with RPi camera }
if(options[2] == 0) {
ret += '1 blue LED (GFP) 0.225 <a href="http://onecall.farnell.com/kingbright/l-10934vbc-ds-d/led-3mm-blue-4000mcd-470nm/dp/2373492?ost=2373492" style="color:#0000bb">Mfr: Kingbright; Part No. L-10934VBC/DS-D</a> 1 550nm dichroic mirror, 25x16mm (GFP) 19.40 <a href="http://www.comaroptics.com/components/filters/dichroic-filters/long-pass-dichroic-filters#row-550_iy_116" style="color:#0000bb">Mfr: Comar; Part No. 550 1Y 116</a> 1 490nm excitation filter, 25x16mm (GFP) 19.40 <a href="http://www.comaroptics.com/components/filters/dichroic-filters/short-pass-dichroic-filters" style="color:#0000bb">Mfr: Comar; Part No. 495 1K 116</a> 1 500nm emission filter, 25x16mm (GFP) 19.40 <a href="http://www.comaroptics.com/components/filters/dichroic-filters/band-pass-dichroic-filters" style="color:#0000bb">Mfr: Comar; Part No. 515 1B 116</a> }
if(options[3] == 0) {
ret += '1 amber LED* (RFP) 1.088 <a href="http://uk.rs-online.com/web/p/visible-leds/8107834/" style="color:#0000bb">Brand: OSRAM Opto Semiconductors; Part No. LY CKBP-JYKX-36-1</a> (must be bought in multiples of 5) 1 dichroic mirror* (RFP) 19.40 <a href="http://www.comaroptics.com/components/filters/dichroic-filters/long-pass-dichroic-filters" style="color:#0000bb">Mfr: Comar; Part No. 650 IY 116</a> 1 excitation filter* (RFP) 19.40 <a href="http://www.comaroptics.com/components/filters/dichroic-filters/long-pass-dichroic-filters" style="color:#0000bb">Mfr: Comar; Part No. 534 IB 116</a> 1 emission filter* (RFP) 19.40 <a href="http://www.comaroptics.com/components/filters/gelatin-and-polyester-colour-filters#row-575_wb_75" style="color:#0000bb">Mfr: Comar; Part No. 575 WB 75</a> }
if(options[4] == 0) {
ret += '5 LEDs tailored to FP 1 dichroic mirror tailored to FP 1 excitation filter tailored to FP 1 emission filter tailored to FP }
if(options[5] == 1 || options[5] == 2) {
ret += '3 stepper motors 3.95 <a href="http://onecall.farnell.com/adafruit-industries/858/stepper-motor-512-step-5vdc/dp/MC02058?ost=MC02058" style="color:#0000bb">Mfr: Adafruit Industries; Part No. 858</a> 2 UNL2003A Darlington driver chips 0.42 <a href="http://uk.rs-online.com/web/p/darlington-transistors/4368451/" style="color:#0000bb">Mfr: Texas Instruments; Part No. ULN2003AN</a> }
if((options[5] == 1 || options[5] == 2) && options[6] == 1) {
ret += '1 9V battery connector 0.172 <a href="http://uk.rs-online.com/web/p/battery-holders-mounts/0489021/" style="color:#0000bb">Brand: RS; Stock No. 489-021</a> }
if((options[5] == 1 || options[5] == 2) && options[6] == 0) {
ret += '2 9V battery connectors 0.172 <a href="http://uk.rs-online.com/web/p/battery-holders-mounts/0489021/" style="color:#0000bb">Brand: RS; Stock No. 489-021</a> }
if(options[6] == 0 && !(options[5] == 1 || options[5] == 2)) {
ret += '1 9V battery connector 0.172 <a href="http://uk.rs-online.com/web/p/battery-holders-mounts/0489021/" style="color:#0000bb">Brand: RS; Stock No. 489-021</a> }
if(options[6] == 0) {
ret += '1 1 Low Profile MoPi power board 25.00 <a href="https://shop.pimoroni.com/products/mopi-mobile-pi-power?utm_medium=cpc&utm_source=googlepla&variant=909042229&gclid=Cj0KEQjwg9-vBRCK7L7wmO2u0JcBEiQA_tzoaMQfkAkb65Xgv6GXoWhTroobFXImuYP392yZocnZEJIaAoYM8P8HAQ" style="color:#0000bb">Product code: MOPI-LP</a> }
if(options[6] == 1) {
ret += '1 2A micro USB power cable 5.66 <a href="http://uk.rs-online.com/web/p/plug-in-power-supply/7653311/" style="color:#0000bb">Mfr: RS; Part No. HNP10I-microUSB</a> }
ret += '</tbody>
'
ret += '
Please note that:
- We haven't managed to get RFP imaging working so the items marked with an asterisk (*) are provided for guidance only\! You will have to find an alternative combination of LED, mirror and filters in order to achieve RFP fluorescence.
- The filters and mirrors are larger than what is needed for a single epicube: if cut carefully into WxD rectangles, a single mirror or filter can be used for N epicubes.
- Only 1 LED is needed per epicube, but we recommend purchasing a few so as to have spares.
- We recommend purchasing 1 RPi camera for each imaging mode (e.g. if you want to image bright-field + GFP + RFP, buy 3 cameras). This is so that you can fix each camera to one cube and leave it there. You CAN buy a single camera for several imaging modes and swap it between cubes, but this will increase the risk of damaging the CCD.
- We provide STL and SCAD files for each 3D printed component so that these can be viewed or edited. When it comes to printing the components, we recommend you print all components in one go, using this file [INCLUDE FILE]. Also, make sure to check out our 3D printing tips [LINK TO SO YOU THINK YOU CAN PRINT]
- If you do not have access to a 3D printer, you can send your files to a 3D printing service and they’ll ship your printed parts.
'
ret += '
Assembly instructions:
- Remove the lens from the Raspberry Pi camera. Using a pair of pliers (the kind with a ridged surface), grip the top circular plastic part of the lens and rotate counter-clockwise. We find it\'s easiest to grip the lens firmly and rotate the sensor, rather than the other way around.
- Using super glue (we recommend Loctite\; apply with a brush) glue the following parts\:
- nuts (x3) on the bottom of the stage
- legs (x1 large + x2 small)
- screw caps (x3)
'
if(options[5] == 0) {
ret += ' - screw holders (x3)
'
}
if(options[5] == 1) {
ret += '- motor holders (x3)
'
}
if(options[5] == 2) {
ret += '- motor holders (x3) or screw holders (x3) depending on whether motorised or manual mode is used
'
}
ret += '- the 2 parts of the z axis together
- RPi lens into lens holder
- supporting axes (x2) into cube holder
- washer into large leg (around the hole)
- Fit the following parts in (you might have to file all holes until they are smooth and large enough...)\:
- slide the main z axis into its slot (the central one) from the bottom, make sure it can move smoothly
- slide the remaining z axis assembly in from top
- make sure the main z axis fits into the slot in the camera holder, if it does - glue it in place
- slide the lens (with its holder) into the camera cube
- click the camera cube into the square hole of the camera holder, make sure it goes all the way down (front face of cube at the level of the bottom surface of the holder)
- detach the camera sensor from the camera board, click the RPi Cam sensor into the camera cube, click the SUNNY back to the board (make sure it stays connected at all times!!!)
- put screws into holes on the top cover, place a washer under each screwtop and screw into the nuts (make sure the superglue has dried...) - now you can move the stage and the z axis and you are ready to image!
'
if(options[5] == 1) {
ret += '- To add the motors: place the camshaft of the motor into the hole designated for it (in the screw cap) and click the motor in - the body should now touch the holder surface, press hard so that it glues in...repeat for all three motors
'
}
if(options[5] == 2) {
ret += '- To use the microscope in motorised mode, add the motors as follows: place the camshaft of the motor into the hole designated for it (in the screw cap) and click the motor in - the body should now touch the holder surface, press hard so that it glues in...repeat for all three motors.
'
}
ret +='
'
return $('').html(ret)
}
step0.bind(step1, [1]);
step1.bind(step2, [0]);
step1.bind(step2, [1]);
step2.bind(step3, [0]);
step2.bind(step3, [1]);
step3.bind(step4, [0]);
step3.bind(step4, [1]);
step4.bind(step5, [0]);
step4.bind(step5, [1]);
step5.bind(step6, [0]);
step5.bind(step6, [1]);
step5.bind(step6, [2]);
step6.bind(final, [0]);
step6.bind(final, [1]);
q.start(step0);
});
.text(this.answers[key].answer)
.data("key", key)
.data("step", this)
.data("quiz", quiz)
.data("index", index)
.click(function(){
key = $(this).data("key");
step = $(this).data("step");
quiz = $(this).data("quiz");
index = $(this).data("index");
ref = quiz.steps[index];
ref.elem.find('.cam-quiz-selected').removeClass('cam-quiz-selected');
$(this).addClass('cam-quiz-selected');
for (var i=quiz.steps.length; --i>index;) {
quiz.steps[i].elem.remove();
quiz.steps.pop();
}
quiz.steps[index].option = key;
nstep = step.answers[key].target;
switch (typeof nstep) {
case "object":
var nindex = quiz.steps.length;
var nref = {
'step': nstep,
'elem': nstep.elem(quiz, nindex),
'option': undefined
};
quiz.steps.push(nref);
quiz.elem.append(nref.elem);
break;
case "function":
options = [];
for (var i=0; i<quiz.steps.length; i++) {
options.push(quiz.steps[i].option);
}
var nref = {
'step': undefined,
'elem': $('')
Here\'s what you\'ll need\:
<thead>
'
ret += '
<thead>
'
ret += '
.append(nstep(options)),
'option': undefined
};
quiz.steps.push(nref);
quiz.elem.append(nref.elem);
break;
default:
alert("Quiz error! Please contact the site maintainers....");
break;
}
}));
}
return elem;
};
for (key in answers) {
answer = answers[key];
this.answers[key] = {
'answer': answer,
'target': undefined
};
}
};
this.start = function(step) {
index = this.steps.length;
ref = {
'step': step,
'elem': step.elem(this, index),
'option': undefined
};
this.steps.push(ref);
this.elem.append(ref.elem);
};
};
$(document).ready(function(){
q = new quiz('#cam-quiz');
step0 = new q.step("Tell us which options you'd like for your microscope.", {
1: "Okay!"
});
step1 = new q.step("Besides bright-field, do you need dark-field imaging?", {
0: "Yes",
1: "No"
});
step2 = new q.step("Do you need to image GFP fluorescence?", {
0: "Yes",
1: "No"
});
step3 = new q.step("Do you need to image RFP fluorescence?", {
0: "Yes",
1: "No"
});
step4 = new q.step("Do you need to image other fluorescent proteins, e.g. YFP?", {
0: "Yes",
1: "No"
});
step5 = new q.step("Do you want your microscope to be manual, motorised, or will you need to swap between both?", {
0: "Manual only",
1: "Motorised only",
2: "Both",
});
step6 = new q.step("Do you need your microscope to be fully battery-powered, e.g. for fieldwork?", {
0: "Yes",
1: "No"
});
final = function(options) {
window.location.href="#CC0";ret = '<h3>Thanks!</h3>
Here\'s what you\'ll need\:
| 3D PRINTED COMPONENTS | |||
|---|---|---|---|
| # | Part | Cost/unit (GBP) | STL & SCAD files |
| 1 | main stage | ||
| 1 | base | ||
| 2 | side supports for z axis | ||
| 1 | z axis in 3 parts (vertical + horizontal + connector) | ||
| 1 | connecting ring bw z axes | ||
| 1 | 3-legged camera holder | ||
| 2 | slide clips | ||
| 1 | bright-field cube | ||
| 1 | dark-field tube in 3 parts | ||
| 3 | epicubes | ||
| 3 | sets of drawers (for filters and mirrors) | ||
| 2 | epicubes | ||
| 2 | sets of drawers (for filters and mirrors) | ||
| 1 | epicube | ||
| 1 | set of drawers (for filters and mirrors) | ||
| 3 | screw gears | ||
| 3 | manual gears | ||
| 3 | screw holders | ||
| 3 | screw gears for motors | ||
| 3 | motor holders | ||
| 1 | battery holder for RPi | ||
| 2 | battery holders for RPi | ||
| 1 | battery holder for RPi | ||
| OFF-THE-SHELF COMPONENTS | |||
|---|---|---|---|
| # | Part | Cost/unit (GBP) | Details & link to provider |
| 1 | white LED (bright-field) | 0.554 | <a href="http://uk.rs-online.com/web/p/visible-leds/7133955/?searchTerm=NSPW500DS&relevancy-data=636F3D3226696E3D4931384E4B6E6F776E41734D504E266C753D656E266D6D3D6D61746368616C6C7061727469616C26706D3D5E5B5C707B4C7D5C707B4E647D2D2C2F255C2E5D2B2426706F3D313326736E3D592673743D4D414E5F504152545F4E554D4245522677633D424F5448267573743D4E535057353030445326" style="color:#0000bb">Mfr: Nichia; Part No. NSPW500DS</a> (must be bought in multiples of 5) |
| 1 | ball lens | 12.20 | <a href="http://www.comaroptics.com/components/lenses/simple-convex-lenses/sapphire-and-glass-ball-lenses#row-03_vq_04" style="color:#0000bb">Mfr: Comar; Part No: 03 VQ 04</a> |
| 2 | 25 mm long M4 screws | 0.107 | Buy at hardware store<a ref="https://www.mackay.co.uk/forgefix-machine-screw-pozi-pan-head-zp-m4-25mm-blister-10-msph425b-screws-fixings-hardware-security-toolbank-brands.html" style="color:#0000bb"> (reference price/unit for a pack of 10)</a> |
| 1 | 40 mm long M4 screw | 0.107 | Buy at hardware store<a ref="https://www.mackay.co.uk/forgefix-machine-screw-pozi-pan-head-zp-m4-40mm-blister-6-msph440b-screws-fixings-hardware-security-toolbank-brands.html" style="color:#0000bb"> (reference price/unit for a pack of 10)</a> |
| 3 | M4 nuts | 0.0043 | Buy at hardware store<a ref="https://www.mackay.co.uk/forgefix-hexagon-nut-zp-m4-bag-100-100nut4-hexagonal-nuts-bolts-fixings-hardware-security-toolbank-brands.html" style="color:#0000bb"> (reference price/unit for a pack of 100)</a> |
| 3 | M4 washers | 0.0037 | Buy at hardware store<a ref="https://www.mackay.co.uk/forgefix-flat-washer-heavyduty-zp-m4-bag-100-100hdwash4-washers-bolts-nuts-fixings-hardware-security-toolbank-brands.html" style="color:#0000bb"> (reference price/unit for a pack of 100)</a> |
| 1 | Raspberry Pi 2 Model B | 26.60 | <a href="http://uk.rs-online.com/web/p/processor-microcontroller-development-kits/832-6274/" style="color:#0000bb">Processor part number: BCM2836</a> |
| 1 | Ralink RT5370 WiFi adapter | 9.10 | <a href="http://www.amazon.co.uk/gp/product/B00IQFGJSS?psc=1&redirect=true&ref_=oh_aui_detailpage_o02_s00" style="color:#0000bb">Amazon Standard Identification Number (ASIN)\: B00IQFGJSS</a> |
| 1 | Arduino UNO with its USB B connector | 21.00 | <a href="https://shop.pimoroni.com/products/arduino-uno" style="color:#0000bb">Mfr: Arduino; Part No. A000066</a> |
| 1 | breadboard | 2.82 | <a href="http://uk.farnell.com/multicomp/mcbb400/breadboard-solderless-300v-abs/dp/2395961?ost=2395961&mckv=ezr2Yisy&CMP=KNC-GUK-FUK-GEN-SHOPPING-MULTICOMP&CAGPSPN=pla&gclid=Cj0KEQjwg9-vBRCK7L7wmO2u0JcBEiQA_tzoaEPR46s_SrENpnwFOv5PS_hqUNVzk8VV5QPxB9AZBwYaAupo8P8HAQ&CAWELAID=120173390000249291&gross_price=true" style="color:#0000bb">Mfr: Multicomp; Part No. MCBB400</a> |
| 1 | 16GB micro SD card | 5.43 | <a href="http://www.amazon.co.uk/SanDisk-Ultra-Imaging-C10-MicroSDHC/dp/B00MBTP8Q4/ref=sr_1_6?ie=UTF8&qid=1442328635&sr=8-6&keywords=micro+sd+16gb+memory+cards" style="color:#0000bb">Model number: SDSDQUIN-016G-G4; ASIN: B00MBTP8Q4</a> |
| 2 | 220 ohm resistors | 0.065 | <a href="http://uk.rs-online.com/web/p/through-hole-fixed-resistors/0131794/" style="color:#0000bb">Brand: TE Connectivity; Part No. CFR100J220R</a> (must be bought in multiples of 10) |
| 1 | ethernet cable | 9.99 | <a href="http://www.pcworld.co.uk/gbuk/computing-accessories/computer-accessories/power-and-cables/computing-cables-and-adaptors/sandstrom-scat65m12-rj45-to-rj45-cat-6-ethernet-cable-5m-12567142-pdt.html" style="color:#0000bb">Mfr: Sandstrøm; Product code: 874926</a> |
| 1 | 100 m spool insulated single-core wire | 13.45 | <a href="http://uk.rs-online.com/web/p/single-core-control-cable/7244311/" style="color:#0000bb">Brand: Lapp; Part No. 4510021</a> |
| 3 | RPi cameras | 22.18 | <a href="http://www.misco.co.uk/product/217272/Raspberry-Pi-NoIR-Camera-Board?fromProductList=Search+result" style="color:#0000bb">Brand: Raspberry Pi; Part No. PiNoir</a> |
| 1 | RPi ribbon cable | 0.00 | Comes with RPi camera |
| 2 | RPi cameras | 22.18 | <a href="http://www.misco.co.uk/product/217272/Raspberry-Pi-NoIR-Camera-Board?fromProductList=Search+result" style="color:#0000bb">Brand: Raspberry Pi; Part No. PiNoir</a> |
| 1 | RPi ribbon cable | 0.00 | Comes with RPi camera |
| 1 | RPi camera | 22.18 | <a href="http://www.misco.co.uk/product/217272/Raspberry-Pi-NoIR-Camera-Board?fromProductList=Search+result" style="color:#0000bb">Brand: Raspberry Pi; Part No. PiNoir</a> |
| 1 | RPi ribbon cable | 0.00 | Comes with RPi camera |
| 1 | blue LED (GFP) | 0.225 | <a href="http://onecall.farnell.com/kingbright/l-10934vbc-ds-d/led-3mm-blue-4000mcd-470nm/dp/2373492?ost=2373492" style="color:#0000bb">Mfr: Kingbright; Part No. L-10934VBC/DS-D</a> |
| 1 | 550nm dichroic mirror, 25x16mm (GFP) | 19.40 | <a href="http://www.comaroptics.com/components/filters/dichroic-filters/long-pass-dichroic-filters#row-550_iy_116" style="color:#0000bb">Mfr: Comar; Part No. 550 1Y 116</a> |
| 1 | 490nm excitation filter, 25x16mm (GFP) | 19.40 | <a href="http://www.comaroptics.com/components/filters/dichroic-filters/short-pass-dichroic-filters" style="color:#0000bb">Mfr: Comar; Part No. 495 1K 116</a> |
| 1 | 500nm emission filter, 25x16mm (GFP) | 19.40 | <a href="http://www.comaroptics.com/components/filters/dichroic-filters/band-pass-dichroic-filters" style="color:#0000bb">Mfr: Comar; Part No. 515 1B 116</a> |
| 1 | amber LED* (RFP) | 1.088 | <a href="http://uk.rs-online.com/web/p/visible-leds/8107834/" style="color:#0000bb">Brand: OSRAM Opto Semiconductors; Part No. LY CKBP-JYKX-36-1</a> (must be bought in multiples of 5) |
| 1 | dichroic mirror* (RFP) | 19.40 | <a href="http://www.comaroptics.com/components/filters/dichroic-filters/long-pass-dichroic-filters" style="color:#0000bb">Mfr: Comar; Part No. 650 IY 116</a> |
| 1 | excitation filter* (RFP) | 19.40 | <a href="http://www.comaroptics.com/components/filters/dichroic-filters/long-pass-dichroic-filters" style="color:#0000bb">Mfr: Comar; Part No. 534 IB 116</a> |
| 1 | emission filter* (RFP) | 19.40 | <a href="http://www.comaroptics.com/components/filters/gelatin-and-polyester-colour-filters#row-575_wb_75" style="color:#0000bb">Mfr: Comar; Part No. 575 WB 75</a> |
| 5 | LEDs | tailored to FP | |
| 1 | dichroic mirror | tailored to FP | |
| 1 | excitation filter | tailored to FP | |
| 1 | emission filter | tailored to FP | |
| 3 | stepper motors | 3.95 | <a href="http://onecall.farnell.com/adafruit-industries/858/stepper-motor-512-step-5vdc/dp/MC02058?ost=MC02058" style="color:#0000bb">Mfr: Adafruit Industries; Part No. 858</a> |
| 2 | UNL2003A Darlington driver chips | 0.42 | <a href="http://uk.rs-online.com/web/p/darlington-transistors/4368451/" style="color:#0000bb">Mfr: Texas Instruments; Part No. ULN2003AN</a> |
| 1 | 9V battery connector | 0.172 | <a href="http://uk.rs-online.com/web/p/battery-holders-mounts/0489021/" style="color:#0000bb">Brand: RS; Stock No. 489-021</a> |
| 2 | 9V battery connectors | 0.172 | <a href="http://uk.rs-online.com/web/p/battery-holders-mounts/0489021/" style="color:#0000bb">Brand: RS; Stock No. 489-021</a> |
| 1 | 9V battery connector | 0.172 | <a href="http://uk.rs-online.com/web/p/battery-holders-mounts/0489021/" style="color:#0000bb">Brand: RS; Stock No. 489-021</a> |
| 1 | 1 Low Profile MoPi power board | 25.00 | <a href="https://shop.pimoroni.com/products/mopi-mobile-pi-power?utm_medium=cpc&utm_source=googlepla&variant=909042229&gclid=Cj0KEQjwg9-vBRCK7L7wmO2u0JcBEiQA_tzoaMQfkAkb65Xgv6GXoWhTroobFXImuYP392yZocnZEJIaAoYM8P8HAQ" style="color:#0000bb">Product code: MOPI-LP</a> |
| 1 | 2A micro USB power cable | 5.66 | <a href="http://uk.rs-online.com/web/p/plug-in-power-supply/7653311/" style="color:#0000bb">Mfr: RS; Part No. HNP10I-microUSB</a> |
Please note that:
- We haven't managed to get RFP imaging working so the items marked with an asterisk (*) are provided for guidance only\! You will have to find an alternative combination of LED, mirror and filters in order to achieve RFP fluorescence.
- The filters and mirrors are larger than what is needed for a single epicube: if cut carefully into WxD rectangles, a single mirror or filter can be used for N epicubes.
- Only 1 LED is needed per epicube, but we recommend purchasing a few so as to have spares.
- We recommend purchasing 1 RPi camera for each imaging mode (e.g. if you want to image bright-field + GFP + RFP, buy 3 cameras). This is so that you can fix each camera to one cube and leave it there. You CAN buy a single camera for several imaging modes and swap it between cubes, but this will increase the risk of damaging the CCD.
- We provide STL and SCAD files for each 3D printed component so that these can be viewed or edited. When it comes to printing the components, we recommend you print all components in one go, using this file [INCLUDE FILE]. Also, make sure to check out our 3D printing tips [LINK TO SO YOU THINK YOU CAN PRINT]
- If you do not have access to a 3D printer, you can send your files to a 3D printing service and they’ll ship your printed parts.
Assembly instructions:
- Remove the lens from the Raspberry Pi camera. Using a pair of pliers (the kind with a ridged surface), grip the top circular plastic part of the lens and rotate counter-clockwise. We find it\'s easiest to grip the lens firmly and rotate the sensor, rather than the other way around.
- Using super glue (we recommend Loctite\; apply with a brush) glue the following parts\:
- nuts (x3) on the bottom of the stage
- legs (x1 large + x2 small)
- screw caps (x3) '
if(options[5] == 0) {ret += ' - screw holders (x3) '
- motor holders (x3) '
- motor holders (x3) or screw holders (x3) depending on whether motorised or manual mode is used '
- the 2 parts of the z axis together
- RPi lens into lens holder
- supporting axes (x2) into cube holder
- washer into large leg (around the hole)
- Fit the following parts in (you might have to file all holes until they are smooth and large enough...)\:
- slide the main z axis into its slot (the central one) from the bottom, make sure it can move smoothly
- slide the remaining z axis assembly in from top
- make sure the main z axis fits into the slot in the camera holder, if it does - glue it in place
- slide the lens (with its holder) into the camera cube
- click the camera cube into the square hole of the camera holder, make sure it goes all the way down (front face of cube at the level of the bottom surface of the holder)
- detach the camera sensor from the camera board, click the RPi Cam sensor into the camera cube, click the SUNNY back to the board (make sure it stays connected at all times!!!)
- put screws into holes on the top cover, place a washer under each screwtop and screw into the nuts (make sure the superglue has dried...) - now you can move the stage and the z axis and you are ready to image!
- To add the motors: place the camshaft of the motor into the hole designated for it (in the screw cap) and click the motor in - the body should now touch the holder surface, press hard so that it glues in...repeat for all three motors
- To use the microscope in motorised mode, add the motors as follows: place the camshaft of the motor into the hole designated for it (in the screw cap) and click the motor in - the body should now touch the holder surface, press hard so that it glues in...repeat for all three motors.
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