Difference between revisions of "Team:HUST-China/Notebook"

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Revision as of 14:18, 17 September 2015

Team:HUST-China:Description


Scroll down to read more


Standalization

Week 1 6.29-7.5

Week 2 7.6-7.12

Week 3 7.13-7.19

Week 4 7.20-7.26

Week 5 7.27-8.2

Week 6 8.3-8.9

PCR:
More details, protocol
WangJie Liu and JunJie Xu



Restriction Enzyme Digestion
WangJie Liu and JunJie Xu


DNA ligation&Transformation
WangJie Liu and JunJie Xu


Successfully align the sequencing results with the objective sequences:
Colony PCR
WangJie Liu and JunJie Xu







Extract the plasmid,Conserving.

Week 7 8.10-8.16

PCR:
More details, protocol
WangJie Liu

Restriction Enzyme Digestion
WangJie Liu


DNA ligation&Transformation
WangJie Liu

Colony PCR
Successfully align the sequencing results with the objective sequences:
WangJie Liu

Extract the plasmid,Conserving.

Week 8 8.17-8.23




Week 9 8.24-8.30

Week 10 8.31-9.6

Successfully align the sequencing results with the objective sequences
Standalization of BD-CRY2 and AD-CIB1 finished.




Successfully align the sequencing results with the objective sequences
Standalization of CRY2 and CIB1 finished.

Light Control

Week 1 6.29-7.5

Week 2 7.6-7.12




Successfully align the sequencing results with the objective sequences of Pgal1 and CYC_TT.
Extract the plasmid.
Conserving.

Week 3 7.13-7.19

Week 4 7.20-7.26




Week 5 7.27-8.2

Successfully align the sequencing results with the objective sequences





Digestion Test
Guozhao Wu

Week 6 8.3-8.9



Successfully align the sequencing results with the objective sequences
Pgal-ROX1-CYC_TT circuit was constructed.

Week 7 8.10-8.16

Week 8 8.17-8.23

Week 9 8.24-8.30

Week 10 8.31-9.6

Viscous Parts

Week 1 6.29-7.5

Week 2 7.6-7.12

PCR:
More details, protocol
Wang Xi and JunJie Xu



Restriction Enzyme Digestion
Wang Xi and JunJie Xu



DNA ligation&Transformation
Wang Xi and JunJie Xu

Colony PCR
Successfully align the sequencing results with the objective sequences:
Wang Xi and JunJie Xu




Extract the plasmid,Conserving

Week 3 7.13-7.19

PCR:
More details, protocol
ZhangYu Cheng



Restriction Enzyme Digestion
ZhangYu Cheng


DNA ligation&Transformation
ZhangYu Cheng

Colony PCR
ZhangYu Cheng
Successfully align the sequencing results with the objective sequences:


Extract the plasmid,Conserving.

Week 4 7.20-7.26

PCR:
More details, protocol
Wang Xi and JunJie Xu

Restriction Enzyme Digestion
Wang Xi and JunJie Xu

DNA ligation&Transformation
Wang Xi and ZhangYu Cheng

Colony PCR
Wang Xi and JunJie Xu
Successfully align the sequencing results with the objective sequences:


Extract the plasmid,Conserving.

Tramsformation to yeast
Lei Yin

Genome extract and PCR test
Lei Yin

Positive strain conservation

Week 5 7.27-8.2

PCR:
More details, protocol
ZhangYu Cheng

Restriction Enzyme Digestion
ZhangYu Cheng

DNA ligation&Transformation
ZhangYu Cheng

Colony PCR
ZhangYu Cheng
Successfully align the sequencing results with the objective sequences:





Extract the plasmid,Conserving.

Trasnsform to yeast

Genome extract and PCR test
Lei Yin

Positive strain conservation

Week 6 8.3-8.9

PCR:
More details, protocol
ZhangYu Cheng

Restriction Enzyme Digestion
ZhangYu Cheng

DNA ligation&Transformation
ZhangYu Cheng

Colony PCR
ZhangYu Cheng
Successfully align the sequencing results with the objective sequences:



Extract the plasmid,Conserving.

Trasnsform to yeast
Lei Yin

Genome extract and PCR test
Lei Yin

Positive strain conservation

Week 7 8.10-8.16

SDS-PAGE
Lei Yin

Immunoflourecense
ZhangYu Cheng

Verification of the secreting Mcfp-3 (Coomassie blue staining)
ZhangYu Cheng

Week 8 8.17-8.23

Week 9 8.24-8.30

Week 10 8.31-9.6

Improvement

Week 1 6.29-7.5

Week 2 7.6-7.12

Week 3 7.13-7.19


Week 4 7.20-7.26

Week 5 7.27-8.2





Week 6 8.3-8.9




Week 7 8.10-8.16

Week 8 8.17-8.23

Week 9 8.24-8.30

Week 10 8.31-9.6

Consolidation

Week 1 6.29-7.5

Week 2 7.6-7.12

Week 3 7.13-7.19

Week 4 7.20-7.26

Week 5 7.27-8.2

Week 6 8.3-8.9

Week 7 8.10-8.16

Week 8 8.17-8.23

Week 9 8.24-8.30

Week 10 8.31-9.6