Difference between revisions of "Team:Warwick/Microscopy"
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| − | <p> This allowed us to use anti-FLAG antibodies to conduct an immuno-fluorescence test to see the location of the protein in the cells. The immuno-fluorescence test is conducted by fixing our modified cells to slides and washing over them with first a primary anti-FLAG antibody, a polypeptide which recognises and binds the FLAG-tag domain we added to our protein, then a secondary antibody that binds to the primary. This secondary antibody is fluorescent. Under a fluorescent microscope this fluorescence should be visible, and it's presence is how we determined qualitatively that the zinc fingers were binding to the surface of the cell. <a href="https:// | + | <p> This allowed us to use anti-FLAG antibodies to conduct an immuno-fluorescence test to see the location of the protein in the cells. The immuno-fluorescence test is conducted by fixing our modified cells to slides and washing over them with first a primary anti-FLAG antibody, a polypeptide which recognises and binds the FLAG-tag domain we added to our protein, then a secondary antibody that binds to the primary. This secondary antibody is fluorescent. Under a fluorescent microscope this fluorescence should be visible, and it's presence is how we determined qualitatively that the zinc fingers were binding to the surface of the cell. <a href="https://static.igem.org/mediawiki/2015/2/2e/WarwickiGEMBacterialProtocolUpdated.pdf">The protocol for this can be found here.</a> </p> |
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<img src="https://static.igem.org/mediawiki/2015/9/91/Warwick_Immunofluorescence_diagram.jpeg" align="middle"> | <img src="https://static.igem.org/mediawiki/2015/9/91/Warwick_Immunofluorescence_diagram.jpeg" align="middle"> | ||
Latest revision as of 15:31, 17 September 2015
