Difference between revisions of "Team:Leicester/Notebook/Week1"

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<p><b>Measurement Study</b></p>
 
<p><b>Measurement Study</b></p>
 
               <p>Use of fluorescence imaging to see the green fluorescence produced from each device. Repeats of each device were produced and placed onto a 96 well plate. First set of rows consist of 200ul of each repeat and next set has 10x dilution.Controls were placed on the bottom row of blank, positive and negative control. Measurements done at about 560nm. </p>
 
               <p>Use of fluorescence imaging to see the green fluorescence produced from each device. Repeats of each device were produced and placed onto a 96 well plate. First set of rows consist of 200ul of each repeat and next set has 10x dilution.Controls were placed on the bottom row of blank, positive and negative control. Measurements done at about 560nm. </p>
−
<p> Nano drops were then taken of repeats which consisted of the highest amount of fluorescence</p>
+
<p> Nano drops were then taken of repeats which consisted of the highest amount of fluorescence. Fluorescence measured using Omega FLUOstar</p>
 
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               <h4 class="timeline-title">22nd June 2015</h4>
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               <h4 class="timeline-title">28th July 2015</h4>
 
             <p><small class="text-muted"><i class="glyphicon glyphicon-time">09:00-18:00</i></small></p>
 
             <p><small class="text-muted"><i class="glyphicon glyphicon-time">09:00-18:00</i></small></p>
 
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               <p><b>Start of Labs</b></p>
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               <p>Meeting about funding and trip to Boston. Measurement study aspects done and mini preps of each device. </p>
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              <p> Making of agar plates with chloramphenicol, ready to used the next day to streak bacteria</p>
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               <h4 class="timeline-title">23rd June 2015</h4>
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               <h4 class="timeline-title">29th July 2015</h4>
 
               <p><small class="text-muted"><i class="glyphicon glyphicon-time">09:00-18:00</i></small></p>
 
               <p><small class="text-muted"><i class="glyphicon glyphicon-time">09:00-18:00</i></small></p>
 
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               <p>Start of Measurement Study</p>
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               <p>Measurement Study</p>
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               <p>-Suspension of parts required for each device required in the measurement study. The parts (Promoter and GFP) were then transformed into DH5(alpha) bacteria. This is then placed on agar plates made the day before. </p>
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               <p>Nano drops to find out concentration of DNA and find out if the right stuff is present</P>  
 
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               <h4 class="timeline-title">24th June 2015</h4>
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               <h4 class="timeline-title">30th July 2015</h4>
 
               <p><small class="text-muted"><i class="glyphicon glyphicon-time">09:00-18:00</i></small></p>
 
               <p><small class="text-muted"><i class="glyphicon glyphicon-time">09:00-18:00</i></small></p>
 
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               <p> Measurement Study</p>
 
               <p> Measurement Study</p>
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               <p>Bacteria produced from the streaks from the previous day are then restreaked again</p>
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               <p>Production of 60 repeats for device 3, these were placed on gel and producing plates</p>
 
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               <h4 class="timeline-title">25 June 2015</h4>
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               <h4 class="timeline-title">31st July 2015</h4>
 
               <p><small class="text-muted"><i class="glyphicon glyphicon-time"> 09:00-18:00</i></small></p>
 
               <p><small class="text-muted"><i class="glyphicon glyphicon-time"> 09:00-18:00</i></small></p>
 
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               <p><b>Measurement Study</b></p>
 
               <p><b>Measurement Study</b></p>
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<p>The restreaks are taken and placed into liquid culture</p>
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<p>Liquid cultures produced and Device 3 re-digested</p>
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<p>Entered for Jamboree</p>
 
              
 
              
 
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Revision as of 15:16, 17 September 2015

Live Preview

Week 1

  • 21 June 2015

    Go fund me page for the Leicester iGEM team was first created as such: Leicester iGEM Go fund me

  • 22nd June 2015

    09:00-18:00

    Start of Labs

    Making of agar plates with chloramphenicol, ready to used the next day to streak bacteria

  • 23rd June 2015

    09:00-18:00

    Start of Measurement Study

    -Suspension of parts required for each device required in the measurement study. The parts (Promoter and GFP) were then transformed into DH5(alpha) bacteria. This is then placed on agar plates made the day before.

  • 24th June 2015

    09:00-18:00

    Measurement Study

    Bacteria produced from the streaks from the previous day are then restreaked again

  • 25 June 2015

    09:00-18:00

    Measurement Study

    The restreaks are taken and placed into liquid culture

Week 2

Week 2

  • 29th June 2015

    09:00-18:00

    Measurement Study

    Mini prep of the liquid cultures were made and then nano drops were done

  • 30th June 2015

    1% agarose gels were made to ensure that the products we wanted were present in solution

Week 3

Due to our project supervisor being away we decided that we concentrated on ensuring that our wiki was started and that we knew what we were doing for Week 4.

Week 4

  • 7th July 2015

    Human Practises

    Have created our synthetic biology survey: Leicester iGEM survey

  • 10th July 2015

    Measurement Study

    Use of fluorescence imaging to see the green fluorescence produced from each device. Repeats of each device were produced and placed onto a 96 well plate. First set of rows consist of 200ul of each repeat and next set has 10x dilution.Controls were placed on the bottom row of blank, positive and negative control. Measurements done at about 560nm.

    Nano drops were then taken of repeats which consisted of the highest amount of fluorescence. Fluorescence measured using Omega FLUOstar

Week 5

  • 21st July 2015

    Human Practises

    Our team T shirts arrived

  • 22nd July 2015

    Measurement Study

    Device 3 experiments were done to ensure that the parts we want are in a given construct, we did this by producing gels and had done some minipreps

  • 23rd July 2015

    09:00-18:00

    Measurement Study

    Liquid cultures produced

  • 24th July 2015

    09:00-18:00

    Sponsored Walk

    Done a sponsored walk to raise some money for travel and accommodation at the Giant Jamboree in Boston. Walked from Market Harborough to Leicester in the lovely rain :)

Week 6

  • 27th July 2015

    9:00-18:00

    Discussion with Professor Ketley about out project in respect to colonisation, etc.

    Look into our wiki and the co

  • 28th July 2015

    09:00-18:00

    Meeting about funding and trip to Boston. Measurement study aspects done and mini preps of each device.

  • 29th July 2015

    09:00-18:00

    Measurement Study

    Nano drops to find out concentration of DNA and find out if the right stuff is present

  • 30th July 2015

    09:00-18:00

    Measurement Study

    Production of 60 repeats for device 3, these were placed on gel and producing plates

  • 31st July 2015

    09:00-18:00

    Measurement Study

    Liquid cultures produced and Device 3 re-digested

    Entered for Jamboree