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We have successfully cloned INP, BclA, and sZFs 2,10 & 14 individually into our construct gene, using the restriction sites we included in the design. Results were confirmed by sequencing colonies picked from antibiotic selection plates.
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We showed that we could bind aminated DNA to slides using a fluorescein-labelled oligonucleotide. Two slides were used: a GOPTS-treated slide that should bind aminated DNA, and a control slide that was cleaned like the GOPTS slide, but was not treated with the GOPTS solution, and should not bind DNA (aminated or otherwise). Our experiment showed fluorescence only on the treated slide, showing that our DNA-immobilisation technique was valid.
