Difference between revisions of "Team:Warwick/Parts"
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| − | + | <p>_______________________________________________________________________________________________________________________________________</p> <h5>Lpp-OmpA-Zif268</h5> | |
<p> | <p> | ||
This part is our construct with Lpp-OmpA as the transmembrane domain and the Zif268 1AAY fragment as the zinc-finger domain. The transmembrane domain can be exchanged with another using NdeI and AgeI, and the zinc-finger domain can be exchanged using NheI and ClaI. The entire construct is under the pL_lac promoter, and contains a C-terminal FLAG tag. | This part is our construct with Lpp-OmpA as the transmembrane domain and the Zif268 1AAY fragment as the zinc-finger domain. The transmembrane domain can be exchanged with another using NdeI and AgeI, and the zinc-finger domain can be exchanged using NheI and ClaI. The entire construct is under the pL_lac promoter, and contains a C-terminal FLAG tag. | ||
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</div> | </div> | ||
<div class="twelve columns noleftmargin"> | <div class="twelve columns noleftmargin"> | ||
| − | + | <p>_______________________________________________________________________________________________________________________________________</p> <h5>Lpp-OmpA-sZF2</h5> | |
<p> | <p> | ||
This part is our construct with Lpp-OmpA as the transmembrane domain and sZF2 as the zinc-finger domain. The transmembrane domain can be exchanged with another using NdeI and AgeI, and the zinc-finger domain can be exchanged using NheI and ClaI. The entire construct is under the pL_lac promoter, and contains a C-terminal FLAG tag. | This part is our construct with Lpp-OmpA as the transmembrane domain and sZF2 as the zinc-finger domain. The transmembrane domain can be exchanged with another using NdeI and AgeI, and the zinc-finger domain can be exchanged using NheI and ClaI. The entire construct is under the pL_lac promoter, and contains a C-terminal FLAG tag. | ||
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</div> | </div> | ||
<div class="twelve columns noleftmargin"> | <div class="twelve columns noleftmargin"> | ||
| − | + | <p>_______________________________________________________________________________________________________________________________________</p> <h5>Lpp-OmpA-sZF10</h5> | |
<p> | <p> | ||
This part is our construct with Lpp-OmpA as the transmembrane domain and sZF10 as the zinc-finger domain. The transmembrane domain can be exchanged with another using NdeI and AgeI, and the zinc-finger domain can be exchanged using NheI and ClaI. The entire construct is under the pL_lac promoter, and contains a C-terminal FLAG tag. | This part is our construct with Lpp-OmpA as the transmembrane domain and sZF10 as the zinc-finger domain. The transmembrane domain can be exchanged with another using NdeI and AgeI, and the zinc-finger domain can be exchanged using NheI and ClaI. The entire construct is under the pL_lac promoter, and contains a C-terminal FLAG tag. | ||
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</div> | </div> | ||
<div class="twelve columns noleftmargin"> | <div class="twelve columns noleftmargin"> | ||
| − | <h5 | + | <p>_______________________________________________________________________________________________________________________________________</p><h5>Lpp-OmpA-sZF14</h5> |
<p> | <p> | ||
This part is our construct with Lpp-OmpA as the transmembrane domain and sZF14 as the zinc-finger domain. The transmembrane domain can be exchanged with another using NdeI and AgeI, and the zinc-finger domain can be exchanged using NheI and ClaI. The entire construct is under the pL_lac promoter, and contains a C-terminal FLAG tag. | This part is our construct with Lpp-OmpA as the transmembrane domain and sZF14 as the zinc-finger domain. The transmembrane domain can be exchanged with another using NdeI and AgeI, and the zinc-finger domain can be exchanged using NheI and ClaI. The entire construct is under the pL_lac promoter, and contains a C-terminal FLAG tag. | ||
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<div class="twelve columns noleftmargin"> | <div class="twelve columns noleftmargin"> | ||
| − | <h5 | + | <p>_______________________________________________________________________________________________________________________________________</p><h5>INP-Zif268</h5> |
<p> | <p> | ||
This part is our construct with INP as the transmembrane domain and the Zif268 1AAY fragment as the zinc-finger domain. The transmembrane domain can be exchanged with another using NdeI and AgeI, and the zinc-finger domain can be exchanged using NheI and ClaI. The entire construct is under the pL_lac promoter, and contains a C-terminal FLAG tag. | This part is our construct with INP as the transmembrane domain and the Zif268 1AAY fragment as the zinc-finger domain. The transmembrane domain can be exchanged with another using NdeI and AgeI, and the zinc-finger domain can be exchanged using NheI and ClaI. The entire construct is under the pL_lac promoter, and contains a C-terminal FLAG tag. | ||
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<div class="twelve columns noleftmargin"> | <div class="twelve columns noleftmargin"> | ||
| − | + | <p>_______________________________________________________________________________________________________________________________________</p> <h5>BclA-Zif268 | |
</h5> | </h5> | ||
<p> | <p> | ||
Revision as of 20:34, 17 September 2015
