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| − | {{:Team:TU_Darmstadt/Templates/CSS}}
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| − | <h3>K1602025 - B0034-HistidinTag-Green fluorescent protein (B0034-His-GFP)</h3>
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| − | <html><div class="contentSection">
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| − | <p align="justify">
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| − | <a href=" https://2015.igem.org/Team:TU_Darmstadt/Notebook/sec1/K1602024 " title="Opens internal link in current window" class="internal link">K1602024</a>
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| − | was digested with <em>Xba</em>I and <em>Pst</em>I and ligated into B0034-pSB1A2. Competent <em>E. coli</em> Top 10 were transformed via heat shock. Colonies were screened by colony PCR with oligonucleotides VF2 and VR. Plasmids of positive clones were extracted. The construct was digested with <em>EcoR</em>I and <em>Pst</em>I and ligated into pSB1C3. Competent <em>E. coli</em> Top 10 were transformed via heat shock.
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| − | Colonies were screened by colony PCR with oligonucleotides VF2 and VR.
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| − | </p>
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| − | </div></html>
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