Difference between revisions of "Team:LZU-China/chz collaborations"

Line 1: Line 1:
 
{{Template:LZU_China/Playground/style}}
 
{{Template:LZU_China/Playground/style}}
 +
<html>
 
<html lang="en">
 
<html lang="en">
 
<head>
 
<head>
Line 26: Line 27:
  
  
   <!-- CSS  
+
   <!-- CSS
   <link href="https://2015.igem.org/Team:LZU-China/chz_materialize.css?action=raw&ctype=text/css" type="text/css" rel="stylesheet" media="screen,projection"/>
+
   <link href="http://7xlqzr.com1.z0.glb.clouddn.com/chzmaterializecss" type="text/css" rel="stylesheet" media="screen,projection"/>
   <link href="https://2015.igem.org/Team:LZU-China/chz_style2.css?action=raw&ctype=text/css" type="text/css" rel="stylesheet" media="screen,projection"/>
+
   <link href="http://7xlqzr.com1.z0.glb.clouddn.com/chzstyle2css" type="text/css" rel="stylesheet" media="screen,projection"/>
 
     -->
 
     -->
  
 
+
<script src="https://2015.igem.org/Team:LZU-China/chz_chart?action=raw&ctype=text/javascript"></script>
 
</head>
 
</head>
 +
 
<body>
 
<body>
 +
 +
 
<header style="margin-top:16px">
 
<header style="margin-top:16px">
 +
<!--<div class="navbar-fixed">-->
 
   <nav class="white" role="navigation">
 
   <nav class="white" role="navigation">
 
     <div class="nav-wrapper container">
 
     <div class="nav-wrapper container">
       <a id="logo-container" href="#" class="brand-logo"><img src="https://static.igem.org/mediawiki/2015/2/2c/Lzu_china_2015_Igem_icon.png" alt="" class="circle responsive-img avat"></a>
+
       <a id="logo-container" href="#" class="brand-logo"><img src="https://static.igem.org/mediawiki/2015/2/2c/Lzu_china_2015_Igem_icon.png" alt="" class="circle responsive-img"></a>
 
       <ul class="right">
 
       <ul class="right">
 
         <li>
 
         <li>
           <a href="https://2015.igem.org/Team:LZU-China/chz index">Main</a>
+
           <a href="https://2015.igem.org/Team:LZU-China/chz_index">Main</a>
 
         </li>
 
         </li>
 
         <li>
 
         <li>
Line 52: Line 57:
 
         </li>
 
         </li>
 
         <li>
 
         <li>
           <a href="#">Attributions</a>
+
           <a href="https://2015.igem.org/Team:LZU-China/Attributions">Attributions</a>
 
         </li>
 
         </li>
 
         <li>
 
         <li>
           <a href="https://2015.igem.org/Team:LZU-China/chz_safety">Safety</a>
+
           <a href=" https://2015.igem.org/Team:LZU-China/chz_safety">Safety</a>
 
         </li>
 
         </li>
 
         <li>
 
         <li>
           <a href="https://2015.igem.org/Team:LZU-China/chz_notes">Notes</a>
+
           <a href=" https://2015.igem.org/Team:LZU-China/chz_notes">Notes</a>
 
         </li>
 
         </li>
 
         <li>
 
         <li>
 
           <a href="https://2015.igem.org/Team:LZU-China/Collaborations">Collaboration</a>
 
           <a href="https://2015.igem.org/Team:LZU-China/Collaborations">Collaboration</a>
 
         </li>
 
         </li>
 
 
       </ul>
 
       </ul>
  
Line 73: Line 77:
 
   </nav>
 
   </nav>
 
</header>
 
</header>
 +
<!--</div>-->
  
  <div id="index-banner" class="parallax-container inorder">
 
    <div class="section no-pad-bot">
 
      <div class="container">
 
        <br><br>
 
        <h1 class="header center teal-text text-lighten-2">Our Team</h1>
 
        <div class="row center">
 
          <h5 class="header col s12 light">A Novel Microbial Fuel Cell System that meaxure the content of estrogen</h5>
 
        </div>
 
 
        <br><br>
 
 
      </div>
 
    </div>
 
    <div class="parallax"><img src="https://static.igem.org/mediawiki/2015/a/a4/Lzu_china_2015_Background1.jpg
 
" alt="Unsplashed background img 2"></div>
 
  </div>
 
 
<div class="row">
 
  
  
 +
<!--Content Start Here-->
 +
<div class="content inorder">
 
   <div class="row">
 
   <div class="row">
     <div class="container">
+
     <div class="col s12 bgbanner">
    <div class="col s12">
+
      <div class="col s12">
      <ul class="tabs">
+
         <div class="col s12 ">
         <li class="tab col s3"><a class="active" href="#attri">Attribution</a></li>
+
          <h5 class="center-align">&nbsp;</h5>
        <li class="tab col s3"><a href="#studen">Students</a></li>
+
          <h2 class="left-align">The collaboration with TJU</h2>
        <li class="tab col s3"><a href="#advis">Instructors</a></li>
+
          <h5 class="left-align"></h5>
        <li class="tab col s3"><a href="#acknow">Acknowledgements</a></li>
+
          <h3 class="center-align">&nbsp;</h3>
      </ul>
+
         </div>
    </div>
+
    </div>
+
     
+
    <div id="attri" class="col s12">
+
        <div class="container">
+
        <table class="centered responsive-table">
+
        <thead>
+
            <tr>
+
                <th>icons</th>
+
                <th>meaning</th>
+
            </tr>
+
            </thead>
+
            <tbody>
+
            <tr>
+
                <td><img style="width:80px" src="https://static.igem.org/mediawiki/2015/c/c9/Lzu_china_2015_Hardwaredesign.png"></td>
+
                <td>Hardware Design</td>
+
            </tr>
+
            <tr>
+
                <td><img style="width:80px" src="https://static.igem.org/mediawiki/2015/a/ac/Lzu_china_2015_Softwaredesign.png"></td>
+
                <td>Software Design</td>
+
            </tr>
+
            <tr>
+
                <td><img style="width:80px" src="https://static.igem.org/mediawiki/2015/b/b5/Lzu_china_2015_Artdesign.png"></td>
+
                <td>Art Design</td>
+
            </tr>
+
            <tr>
+
                <td><img style="width:80px" src="https://static.igem.org/mediawiki/2015/9/93/Lzu_china_2015_MFC_experiment.png"></td>
+
                <td>MFC experiment</td>
+
            </tr>
+
            <tr>
+
                <td><img style="width:80px" src="https://static.igem.org/mediawiki/2015/2/25/Lzu_china_2015_Devicerunning.png"></td>
+
                <td>Device running</td>
+
            </tr>
+
            <tr>
+
                <td><img style="width:80px" src="https://static.igem.org/mediawiki/2015/1/13/Lzu_china_2015_Experiment_design.png"></td>
+
                <td>Experiment Design</td>
+
            </tr>
+
            <tr>
+
                <td><img style="width:80px" src="https://static.igem.org/mediawiki/2015/b/b5/Lzu_china_2015_Humanpractice.png"></td>
+
                <td>HumanPractice</td>
+
            </tr>
+
            <tr>
+
                <td><img style="width:80px" src="https://static.igem.org/mediawiki/2015/9/99/Lzu_china_2015_Paperwork.png"></td>
+
                <td>PaperWork</td>
+
            </tr>
+
            <tr>
+
                <td><img style="width:80px" src="https://static.igem.org/mediawiki/2015/0/09/Lzu_china_2015_Part_construction.png"></td>
+
                <td>Part construction</td>
+
            </tr>
+
            <tr>
+
                <td><img style="width:80px" src="https://static.igem.org/mediawiki/2015/3/30/Lzu_china_2015_Partexamination.png"></td>
+
                <td>Part Examination</td>
+
            </tr>
+
            <tr>
+
                <td><img style="width:80px" src="https://static.igem.org/mediawiki/2015/b/b0/Lzu_china_2015_Teammanagement.png"></td>
+
                <td>Team management</td>
+
            </tr>
+
            <tr>
+
                <td><img style="width:80px" src="https://static.igem.org/mediawiki/2015/3/35/Lzu_china_2015_Wikicode.png"></td>
+
                <td>Wiki Code</td>
+
            </tr>
+
           
+
            </tbody>
+
         </table>
+
 
       </div>
 
       </div>
 
     </div>
 
     </div>
    <div id="studen" class="col s12">
 
     
 
<div class="col s12" style="background-color: rgb(187, 180, 164);">
 
  <div class="col s12 m12 l4">
 
    <img src="https://static.igem.org/mediawiki/2015/c/c5/Lzu_china_2015_Lzuzhangxiao.JPG
 
" class="responsive-img avat">
 
 
   </div>
 
   </div>
  <div class="col s12 m12 l6">
+
</div>
    <div class="row">
+
      <h4>Xiao Zhang</h4>
+
      <p>Xiao Zhang majoring in bioinformatics and microbiology is a master degree candidate studying in State Key Laboratory of Grassland Agro-ecosystem, Lanzhou University. As the captain of our team and the co-inventor of our novel device, he conceived and designed the entire MFC system. His versatility has great value for us to finish our project. He is really keen on science research, especially interested in functional genomics. So far, he has taken part in several research topics, and their scientific achievements were reported in 3 different SCI papers.
+
      </p>
+
        <p>ATTRIBUTION:</p>
+
        <div class="col s12">
+
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/c/c9/Lzu_china_2015_Hardwaredesign.png">
+
<img style="width:50px" src="https://static.igem.org/mediawiki/2015/a/ac/Lzu_china_2015_Softwaredesign.png">
+
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/2/25/Lzu_china_2015_Devicerunning.png">
+
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/b/b0/Lzu_china_2015_Teammanagement.png">
+
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/b/b5/Lzu_china_2015_Humanpractice.png">
+
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/1/13/Lzu_china_2015_Experiment_design.png">
+
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/99/Lzu_china_2015_Paperwork.png">
+
        </div>
+
    </div>
+
  </div>
+
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(187, 180, 164);">
+
      <h1>&nbsp;</h1>
+
      <ul>
+
      <li>OverGraduate</li>
+
      <li>UnderGraudate</li>
+
      </ul>
+
  </div>
+
</div><!--L1-->
+
   
+
<div class="col s12" style="background-color: rgb(254, 123, 175);">
+
<div class="col s12 m12 l2 teamblock" style="background-color: rgb(254, 123, 175);">
+
 
+
      <h1>&nbsp;</h1>
+
      <ul>
+
      <li>OverGraduate</li>
+
      <li>UnderGraudate</li>
+
      </ul>
+
  </div>
+
  <div class="col s12 m12 l6">
+
    <div class="row">
+
      <h4>Qi Wu</h4>
+
      <p>Chariness patience and happiness, she is Qi wu, a second times iGEMer. As co-captain this year, She is in charge of logistics and finance. This is a totally different experience from last year and she learned a lot.
+
      </p>
+
      <br/>
+
          <p>ATTRIBUTION:</p>
+
          <div>                   
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/0/09/Lzu_china_2015_Part_construction.png">
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/3/30/Lzu_china_2015_Partexamination.png">
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/b/b0/Lzu_china_2015_Teammanagement.png">
+
        </div>
+
    </div>
+
  </div>
+
    <div class="col s12 m12 l4">
+
    <img src="https://static.igem.org/mediawiki/2015/a/a9/Lzu_china_2015_Lzuwuqi.jpg
+
" class="responsive-img avatr">
+
  </div>
+
 
+
</div><!--R2-->
+
 
+
<div class="col s12" style="background-color: rgb(148, 188, 92);">
+
  <div class="col s12 m12 l4">
+
    <img src="https://static.igem.org/mediawiki/2015/0/0c/Lzu_china_2015_Lzuwanghaotian.jpg
+
" class="responsive-img avat">
+
  </div>
+
  <div class="col s12 m12 l6">
+
    <div class="row">
+
      <h4>Haotian Wang</h4>
+
      <p>Haotian Wang is a junior student majoring in bioscience. He is a fan of Liverpool F. C. Being a good striker used to be his dream while he is now a sideback. He is so interested in synthetic biology that he always reads some articles before watching games. He constructed some of our parts.
+
      </p>
+
       
+
        <p>ATTRIBUTION:</p>
+
        <div class="col s12">
+
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/0/09/Lzu_china_2015_Part_construction.png">
+
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/3/30/Lzu_china_2015_Partexamination.png">
+
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/1/13/Lzu_china_2015_Experiment_design.png">
+
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/99/Lzu_china_2015_Paperwork.png">
+
        </div>
+
    </div>
+
  </div>
+
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(148, 188, 92);">
+
      <h1>&nbsp;</h1>
+
      <ul>
+
      <li>OverGraduate</li>
+
      <li>UnderGraudate</li>
+
      </ul>
+
  </div>
+
</div><!--L3-->
+
 
+
<div class="col s12" style="background-color: rgb(197, 193, 189);">
+
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(197, 193, 189);">
+
      <h1>&nbsp;</h1>
+
      <ul>
+
      <li>OverGraduate</li>
+
      <li>UnderGraudate</li>
+
      </ul>
+
  </div>
+
  <div class="col s12 m12 l6">
+
    <div class="row">
+
      <h4>Xuguo Wang</h4>
+
      <p>Xuguo,Wang.As people say, guy who learning computer always a geek, yes, he is.
+
He is a graduate student of Lanzhou University, he is learning linux kernel,
+
besides this, he likes making some interesting electronical device, and like Quadcopter,
+
like transform the Raspberry Pi to control his home's something. Well, that is very
+
interesting. He is dreaming to contribute the linux kernel code, and working
+
for this. He is a keen on computer algorithm, and hope someday, can make his own
+
algorithm.
+
      </p>
+
      <br/>
+
        <p>ATTRIBUTION:</p>
+
        <div>             
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/c/c9/Lzu_china_2015_Hardwaredesign.png">
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/a/ac/Lzu_china_2015_Softwaredesign.png">
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/2/25/Lzu_china_2015_Devicerunning.png">
+
<img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/99/Lzu_china_2015_Paperwork.png">
+
        </div>
+
    </div>
+
  </div>
+
    <div class="col s12 m12 l4">
+
    <img src="https://static.igem.org/mediawiki/2015/9/96/Lzu_china_2015_Lzuwangxuguo.jpg
+
" class="responsive-img avatr">
+
  </div>
+
 
+
</div><!--R4-->
+
 
+
<div class="col s12" style="background-color: rgb(189, 212, 253);">
+
  <div class="col s12 m12 l4">
+
    <img src="https://static.igem.org/mediawiki/2015/d/dc/Lzu_china_2015_Lzuchenzhengjun.jpg
+
" class="responsive-img avat">
+
  </div>
+
  <div class="col s12 m12 l6">
+
    <div class="row">
+
      <h4>Zhengjun Chen</h4>
+
      <p>(1985- ), male, is studying at Lanzhou University and pursuing a doctor's degree in microbiology. His main research interests are environmental microorganisms, and two papers have been published in SCI. He is also an adviser of iGEM team of Lanzhou University.
+
      </p>
+
        <p>ATTRIBUTION:</p>
+
        <div class="col s12">
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/c/c9/Lzu_china_2015_Hardwaredesign.png">
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/93/Lzu_china_2015_MFC_experiment.png">
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/1/13/Lzu_china_2015_Experiment_design.png">
+
        </div>
+
    </div>
+
  </div>
+
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(189, 212, 253);">
+
      <h1>&nbsp;</h1>
+
      <ul>
+
      <li>OverGraduate</li>
+
      <li>UnderGraudate</li>
+
      </ul>
+
  </div>
+
</div><!--L5-->
+
       
+
<div class="col s12" style="background-color: rgb(240, 205, 165);">
+
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(240, 205, 165);">
+
      <h1>&nbsp;</h1>
+
      <ul>
+
      <li>OverGraduate</li>
+
      <li>UnderGraudate</li>
+
      </ul>
+
  </div>
+
  <div class="col s12 m12 l6">
+
    <div class="row">
+
      <h4>Rong Xu</h4>
+
      <p>born in July 1987, is a doctor candidate studying in Lanzhou University. She is an expert of heavy metal reduction and cellulose degradation using gene modified bacteria and she does enjoy her researches. Moreover, she helps us with her enthusiasm overcome many kinds of problems about our project. So we believe that she is one of the best advisors in our team-LZU CHINA to attend this competition.
+
      </p>
+
      <br/>
+
        <p>ATTRIBUTION:</p>
+
        <div>                   
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/0/09/Lzu_china_2015_Part_construction.png">
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/3/30/Lzu_china_2015_Partexamination.png">
+
        </div>
+
    </div>
+
  </div>
+
    <div class="col s12 m12 l4">
+
    <img src="https://static.igem.org/mediawiki/2015/3/3f/LZU_China_Team_xurong.jpg" class="responsive-img avatr">
+
  </div>
+
 
+
</div><!--R6-->
+
 
+
<div class="col s12" style="background-color: rgb(81, 212, 90);">
+
  <div class="col s12 m12 l4">
+
    <img src="https://static.igem.org/mediawiki/2015/thumb/b/bd/LZU_China_Tean_duxuejing.jpg/600px-LZU_China_Tean_duxuejing.jpg" class="responsive-img avat">
+
  </div>
+
  <div class="col s12 m12 l6">
+
    <div class="row">
+
      <h4>Xuejing Du</h4>
+
      <p>Xuejing Du is one of the student leader of group members in our project. His interest is microbiology and behavioural sciences. It is he who has recruited the key members of this team and has brought a new concept to MFCs. He not only wrote over the 80% of the paperwork but also has done 90% of the artwork for wiki, poster and uniform. Human practice, which we thought a tough, sophisticated burden, was carried by him. In addition, he has designed a questionnaire and made a survey about people's attitude towards sewage treatment and the use of microbial fuel cells in China and UK.His creative ideas help us vastly. Now, he is preparing for the presentation in order to help our team to attend Giant Jamboree in October.
+
      </p>
+
        <p>ATTRIBUTION:</p>
+
        <div class="col s12">
+
            <img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/99/Lzu_china_2015_Paperwork.png">
+
            <img style="width:50px" src="https://static.igem.org/mediawiki/2015/b/b5/Lzu_china_2015_Artdesign.png">
+
            <img style="width:50px" src="https://static.igem.org/mediawiki/2015/b/b5/Lzu_china_2015_Humanpractice.png">
+
        </div>
+
    </div>
+
  </div>
+
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(81, 212, 90);">
+
      <h1>&nbsp;</h1>
+
      <ul>
+
      <li>OverGraduate</li>
+
      <li>UnderGraudate</li>
+
      </ul>
+
  </div>
+
</div><!--L7-->
+
  
 
<div class="col s12">
 
<div class="col s12">
  <div class="col s12 m12 l2 teamblock">
+
<div class="container">
      <h1>&nbsp;</h1>
+
     <p> We started our contact with team TJU from the very beginning of the summer. Since both teams are working on the MFC system, we have shared a lot of experience on it, including the MFC device setup, medium condition control and part characterization. Additionally, we had lots of online and offline discussion with them.  
      <ul>
+
    </p>
      <li>OverGraduate</li>
+
     <br/>
      <li>UnderGraudate</li>
+
     <p>In the middle of the project, we came to Key Laboratory of Systems Bioengineering (Tianjin University) to have a project seminar with them. During the discussion, we knew that they have constructed a lactate producing part named ldhE. However, their characterization of this part was faced with some problem. To be specific, engineered E.coli strain bearing the ldhE part showed little difference with the wild type. (shown as follows)</p>
      </ul>
+
  </div>
+
  <div class="col s12 m12 l6">
+
     <div class="row">
+
      <h4>Yaxin Pei</h4>
+
      <p>born in July 1987, is a doctor candidate studying in Lanzhou University. She is an expert of heavy metal reduction and cellulose degradation using gene modified bacteria and she does enjoy her researches. Moreover, she helps us with her enthusiasm overcome many kinds of problems about our project. So we believe that she is one of the best advisors in our team-LZU CHINA to attend this competition.
+
      </p>
+
      <br/>
+
        <div>                 
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/b/b5/Lzu_china_2015_Humanpractice.png">
+
        </div>
+
    </div>
+
  </div>
+
    <div class="col s12 m12 l4">
+
    <img src="img/p/lzuxurong.jpg" class="responsive-img avatr">
+
  </div>
+
 
+
</div><!--R8-->
+
       
+
<div class="col s12" style="background-color: rgb(185, 236, 237);">
+
  <div class="col s12 m12 l4">
+
    <img src="https://static.igem.org/mediawiki/2015/c/cd/Lzu_china_2015_Lzuzhangzhengdong.jpg
+
" class="responsive-img avat">
+
  </div>
+
  <div class="col s12 m12 l6">
+
    <div class="row">
+
      <h4>Zhengdong Zhang</h4>
+
      <p>Amongst all the members struggling for our project, I rank as those busiest guys. My name is Zhengdong Zhang, working like a dog, dressed as a dull nerd. Not am I clever, but the reason why I had been busy was just I have few hobby. Like many of our team, biology science is my only lover.
+
      </p>
+
        <p>ATTRIBUTION:</p>
+
        <div class="col s12">
+
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/0/09/Lzu_china_2015_Part_construction.png">
+
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/3/30/Lzu_china_2015_Partexamination.png">
+
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/99/Lzu_china_2015_Paperwork.png">
+
        </div>
+
     </div>
+
  </div>
+
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(185, 236, 237);">
+
      <h1>&nbsp;</h1>
+
      <ul>
+
      <li>OverGraduate</li>
+
      <li>UnderGraudate</li>
+
      </ul>
+
  </div>
+
</div><!--L9-->
+
       
+
<div class="col s12" style="background-color: rgb(171, 170, 80);">
+
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(171, 170, 80);">
+
      <h1>&nbsp;</h1>
+
      <ul>
+
      <li>OverGraduate</li>
+
      <li>UnderGraudate</li>
+
      </ul>
+
  </div>
+
  <div class="col s12 m12 l6">
+
     <div class="row">
+
      <h4>Feifan Xu</h4>
+
      <p>My name is Feifan Xu, a junior student. It is my great honor to become part of iGEM LZU-China 2015. To be honest, I am a diffident guy more or less. So work like electrophoresis PCR or other molecule experiment are quite suitable for me. My primary job is parts construction. Besides that, the measurement of part expression quantity and a little MFC work is also my duty. I like swim in my free time. I love computer games too. After the experiment of the passing year, I find out a truth. The only failure is give up, if you keep fighting, hope is coming. 
+
      </p>
+
      <br/>
+
        <p>ATTRIBUTION:</p>
+
        <div>               
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/0/09/Lzu_china_2015_Part_construction.png">
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/3/30/Lzu_china_2015_Partexamination.png">
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/99/Lzu_china_2015_Paperwork.png">
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/3/35/Lzu_china_2015_Wikicode.png">
+
        </div>
+
    </div>
+
  </div>
+
    <div class="col s12 m12 l4">
+
    <img src="https://static.igem.org/mediawiki/2015/1/1a/Lzu_china_2015_Lzuxufeifan.jpg
+
" class="responsive-img avatr">
+
  </div>
+
 
+
</div><!--R10-->
+
       
+
<div class="col s12" style="background-color: rgb(189, 193, 194);">
+
  <div class="col s12 m12 l4">
+
    <img src="https://static.igem.org/mediawiki/2015/3/3e/Lzu_china_2015_Lzudengfei.jpg
+
" class="responsive-img avat">
+
  </div>
+
  <div class="col s12 m12 l6">
+
    <div class="row">
+
      <h4>Fei Deng</h4>
+
      <p>Fei Deng majors in biology and he seems like a serious guy. Indeed, He is strict with lots of things, such as the operations of our experiments. But he is an easygoing man in daily life. He likes a lots of activities including playing football, reading books. While he said that he cannot tell us what he loves most. In this program, he constructed some parts such as part K1755003 and examined some parts to verify that they can really work. Besides, he wrote some text for our wiki.  
+
      </p>
+
        <p>ATTRIBUTION:</p>
+
        <div class="col s12">
+
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/93/Lzu_china_2015_MFC_experiment.png">
+
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/3/30/Lzu_china_2015_Partexamination.png">
+
        </div>
+
    </div>
+
  </div>
+
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(189, 193, 194);">
+
      <h1>&nbsp;</h1>
+
      <ul>
+
      <li>OverGraduate</li>
+
      <li>UnderGraudate</li>
+
      </ul>
+
  </div>
+
</div><!--L11-->
+
       
+
<div class="col s12" style="background-color: rgb(147, 188, 210);">
+
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(147, 188, 210);">
+
      <h1>&nbsp;</h1>
+
      <ul>
+
      <li>OverGraduate</li>
+
      <li>UnderGraudate</li>
+
      </ul>
+
  </div>
+
  <div class="col s12 m12 l6">
+
    <div class="row">
+
      <h4>Liu Yang</h4>
+
      <p>Her name is Liu Yang, a junior student majoring in life science. As an outgoing girl, she likes travelling in nature. With the ardent love of synthesis biology, she constructed the part and detected its work in MFC for our team this year. Although she failed a lot, she never gave up but continued working and eventually overcame all the tough things. She’s a girl of real strength.
+
      </p>
+
      <br/>
+
        <p>ATTRIBUTION:</p>
+
        <div>                 
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/0/09/Lzu_china_2015_Part_construction.png">
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/3/30/Lzu_china_2015_Partexamination.png">
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/99/Lzu_china_2015_Paperwork.png">
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/93/Lzu_china_2015_MFC_experiment.png">
+
        </div>
+
    </div>
+
  </div>
+
    <div class="col s12 m12 l4">
+
    <img src="https://static.igem.org/mediawiki/2015/2/20/Lzu_china_2015_Lzuyangliu.jpg
+
" class="responsive-img avatr">
+
  </div>
+
 
+
</div><!--R12-->
+
       
+
<div class="col s12" style="background-color: rgb(187, 36, 25);">
+
  <div class="col s12 m12 l4">
+
    <img src="https://static.igem.org/mediawiki/2015/4/45/Lzu_china_2015_Lzuyaomengna.jpg
+
" class="responsive-img avat">
+
  </div>
+
  <div class="col s12 m12 l6">
+
    <div class="row">
+
      <h4>Mengna Yao</h4>
+
      <p>Mengna Yao is a junior student who loves life sciences so much that she ‘s always happy as long as in lab. She did some parts’ work , made booklets and assist in designing poster and wiki. She likes watching anime, being eager for anything unusual. She’s a patient and honest girl, mild and energetic.
+
      </p>
+
        <p>ATTRIBUTION:</p>
+
        <div class="col s12">
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/0/09/Lzu_china_2015_Part_construction.png">
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/99/Lzu_china_2015_Paperwork.png">
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/b/b5/Lzu_china_2015_Artdesign.png">       
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/2/25/Lzu_china_2015_Devicerunning.png">
+
       
+
        </div>
+
    </div>
+
  </div>
+
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(187, 36, 25);">
+
      <h1>&nbsp;</h1>
+
      <ul>
+
      <li>OverGraduate</li>
+
      <li>UnderGraudate</li>
+
      </ul>
+
  </div>
+
</div><!--L13-->
+
       
+
<div class="col s12" style="background-color: rgb(201, 201, 199);">
+
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(201, 201, 199);">
+
      <h1>&nbsp;</h1>
+
      <ul>
+
      <li>OverGraduate</li>
+
      <li>UnderGraudate</li>
+
      </ul>
+
  </div>
+
  <div class="col s12 m12 l6">
+
    <div class="row">
+
      <h4>Yu Chen</h4>
+
      <p>Yu Chen, undergraduate student of biology in Lanzhou University, has a great enthusiasm in doing experiment. As a member of the 2015 iGEM team of Lanzhou University, he devotes himself into making parts and MFC. He likes reading, film and animation, he also likes to play table tennis with his friends in his free time.
+
  
      </p>
+
    <div class="col s12 row">
      <br/>
+
              <div class="col s10 m6 l6">
        <p>ATTRIBUTION:</p>
+
                <div class="card">
        <div>                  
+
                <div class="card-image">
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/0/09/Lzu_china_2015_Part_construction.png">
+
                  <img src="https://static.igem.org/mediawiki/2015/e/e9/LZU_China_Notes_3-1.collobration_1.png" >
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/3/30/Lzu_china_2015_Partexamination.png">
+
                  <span class="card-title"></span>
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/99/Lzu_china_2015_Paperwork.png">
+
                </div>
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/3/35/Lzu_china_2015_Wikicode.png">
+
                <div class="card-content">
        </div>
+
                  <p>Fig.1 The production of lactate in MG1665-WT. The cells were cultured in 37C for 15h and the lactate concentration was measured by HPLC.</p>
     </div>
+
                </div>
  </div>
+
                <!--<div class="card-action">
     <div class="col s12 m12 l4">
+
                  <a href="#">This is a link</a>
    <img src="https://static.igem.org/mediawiki/2015/0/09/Lzu_china_2015_Lzuchenyu.jpg" class="responsive-img avatr">
+
                </div>-->
  </div>
+
              </div>
 
+
              </div>
</div><!--R14-->
+
          </div>
       
+
    <br/>
<div class="col s12" style="background-color: rgb(247, 214, 176);">
+
    <p>For the lactate producing system, we have helped team TJU to optimize and characterize the ldhE part. From their introduction, we know that LDH is an enzyme catalyzes the conversion of pyruvate to lactate with NADH serving as the coenzyme. Initially, they intended to introduce high-yield u L-(+)-lactate dehydrogenase gene (ldhA) from Lactobacillus, which could produce a relatively larger amount of L-lactate for Shewanella. Consequently, we decided to focus on the assistant method to help them resolve the problem.</p>
  <div class="col s12 m12 l4">
+
    <p>
    <img src="https://static.igem.org/mediawiki/2015/a/af/Lzu_china_2015_Lzuyangmingchu.jpg
+
    After some consultant, we noticed that in the wild type E. coli, LDH reaction is not as competitive as the reaction through PFL, which might result in the ineffectiveness of ldhE.[1] Therefore, knockout of the PFL related genes will contribute to redistribute the metabolic flux. In addition, we searched in the part registry of IGEM and found the part BBa_K341458 and part BBa_K341002, which revealed a more effective and easy-to-use method for gene knockout. 
" class="responsive-img avat">
+
    </p>
  </div>
+
    <br/>
  <div class="col s12 m12 l6">
+
     <p>In the system we searched, the cell is first transformed with a helper plasmid harboring genes encoding the λ-Red enzymes, I-SceI endonuclease, and RecA. λ-Red enzymes expressed from the helper plasmids are used to recombine a small ‘landing pad’, a tetracycline resistance gene (tetA) flanked by I-SceI recognition sites and landing pad regions, into the desired location in the chromosome. After tetracycline selection for successful landing pad integrants, the cell is transformed with a donor plasmid carrying the desired insertion fragment; this fragment is excised by I-SceI and incorporated into the landing pad via recombination at the landing pad regions.[2] We think this more effective and easy method would help them overcome the problem. So, we recommended TJU to adopt the two-step λ-Red method to knock out the pflB gene.</p>
    <div class="row">
+
    <br/>
      <h4>Mingchu Yang</h4>
+
     <p>
      <p>My name is Mingchu Yang. In China “YANGMING” mean “famous”. And if you read my name upside down “CHUMING” mean famous too. So my dad gave me the name of a certain hope I can have a wonderful future. This time in Igem, I gain a lot. Not only increased my knowledge also strengthened the ability experiment. Above all, in this team, I got the most important thing. That is friends.  
+
    After their reconstruction of the engineered strain, a more visible contrast was shown as below.
 +
    </p>
 +
    <div class="col s12 row">
 +
              <div class="col s10 m6 l6">
 +
                <div class="card">
 +
                <div class="card-image">
 +
                  <img src="https://static.igem.org/mediawiki/2015/thumb/3/32/LZU_China_Notes_3-1.collobration_2.png/800px-LZU_China_Notes_3-1.collobration_2.png" >
 +
                  <span class="card-title"></span>
 +
                </div>
 +
                <div class="card-content">
 +
                  <p>Fig.2 The production of lactate of the MG1655ΔpflB. The cells are cultured in 37C. (Data from TJU)</p>
 +
                </div>
 +
                <!--<div class="card-action">
 +
                  <a href="#">This is a link</a>
 +
                </div>-->
 +
              </div>
 +
              </div>
 +
          </div>
 +
    <br/>
 +
    <p><h6>
 +
      In order to prove the cell can work normally, we tested the knock-out cell’s field of lactate in our lab. The result is shown in the figure below.
 +
    </h6></p>
 +
    <div class="col s12 row">
 +
              <div class="col s10 m6 l6">
 +
                <div class="card">
 +
                <div class="card-image">
 +
                  <img src="https://static.igem.org/mediawiki/2015/4/40/LZU_China_Notes_3-1.collobration_3.png" >
 +
                  <span class="card-title"></span>
 +
                </div>
 +
                <div class="card-content">
 +
                  <p>Fig.3 The production of lactate of the MG1655ΔpflB. The cells are cultured in 37C for 26h. (data from LZU-China)</p>
 +
                </div>
 +
                <!--<div class="card-action">
 +
                  <a href="#">This is a link</a>
 +
                </div>-->
 +
              </div>
 +
              </div>
 +
          </div>
 +
    <br/>
 +
    <p><h5><strong>References</strong></h5></p>
 +
    <p><h6>
 +
      [1]Zhu J, Shimizu K, Zhu J, et al. Effect of a single-gene knockout on the metabolic regulation in Escherichia coli for D-lactate production under microaerobic condition[J]. Metabolic Engineering, 2005, 7(2):104–115.
 +
    </h6></p>
 +
    <p><h6>
 +
      [2]Thomas E, Kuhlman, Edward C, Cox. Site-specific chromosomal integration of large synthetic constructs.[J]. Nucleic Acids Research, 2010, 38(38).
 +
    </h6></p>
  
      </p>
 
        <p>ATTRIBUTION:</p>
 
        <div class="col s12">
 
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/b/b5/Lzu_china_2015_Artdesign.png">
 
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/99/Lzu_china_2015_Paperwork.png">
 
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/b/b5/Lzu_china_2015_Humanpractice.png">
 
        </div>
 
    </div>
 
  </div>
 
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(247, 214, 176);">
 
      <h1>&nbsp;</h1>
 
      <ul>
 
      <li>OverGraduate</li>
 
      <li>UnderGraudate</li>
 
      </ul>
 
  </div>
 
</div><!--L15-->
 
       
 
<div class="col s12" style="background-color: rgb(187, 119, 96);">
 
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(187, 119, 96);">
 
      <h1>&nbsp;</h1>
 
      <ul>
 
      <li>OverGraduate</li>
 
      <li>UnderGraudate</li>
 
      </ul>
 
  </div>
 
  <div class="col s12 m12 l6">
 
    <div class="row">
 
      <h4>Huzi Cheng</h4>
 
      <p>Huzi Cheng designed both the visual looks and logical struct of our wiki. He also in charge of  building, uploading and making sure it can works well and being attractive to viewers.He designed our annual logo ,also.He joined the visualization and the fitting of our data.
 
  
      </p>
 
      <br/>
 
      <p>ATTRIBUTION:</p>
 
        <div>               
 
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/b/b5/Lzu_china_2015_Artdesign.png">
 
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/3/35/Lzu_china_2015_Wikicode.png">
 
        </div>
 
    </div>
 
  </div>
 
    <div class="col s12 m12 l4">
 
    <img src="https://static.igem.org/mediawiki/2015/thumb/2/2a/LZUChenghuzi.jpg/576px-LZUChenghuzi.jpg" class="responsive-img avatr">
 
  </div>
 
 
 
</div><!--R16-->
 
       
 
<div class="col s12" style="background-color: rgb(238, 238, 223);">
 
  <div class="col s12 m12 l4">
 
    <img src="https://static.igem.org/mediawiki/2015/1/1e/Lzu_china_2015_Lzushaotianyi.jpg
 
" class="responsive-img avat">
 
  </div>
 
  <div class="col s12 m12 l6">
 
    <div class="row">
 
      <h4>Tianyi Shao</h4>
 
      <p>Hello,I am Tianyi Shao majoring in Chemistry from Lanzhou University.I don’t mean to exaggerate how splendidly our project performs in that Micro-Holmes as a pollutant detector is pragmatic and useful in every corner of this contaminated world.Three other partners and I are primarily in charge of the construction and operation of the MFCs,and collecting the data for an experienced formula that has widely applicability.I went to Japan for communication with top Japanese iGEM’s undergraduates in this summer and we all enjoyed this kind of opportunities to share our precious ideas.I am looking forward to having a brainstorm with you coming from all over the world in Boston.
 
      </p>
 
        <p>ATTRIBUTION:</p>
 
        <div class="col s12">
 
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/3/30/Lzu_china_2015_Partexamination.png">
 
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/93/Lzu_china_2015_MFC_experiment.png">
 
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/b/b5/Lzu_china_2015_Humanpractice.png">
 
        </div>
 
    </div>
 
  </div>
 
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(238, 238, 223);">
 
      <h1>&nbsp;</h1>
 
      <ul>
 
      <li>OverGraduate</li>
 
      <li>UnderGraudate</li>
 
      </ul>
 
  </div>
 
</div><!--L17-->
 
       
 
<div class="col s12" style="background-color: rgb(115, 151, 213);">
 
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(115, 151, 213);">
 
      <h1>&nbsp;</h1>
 
      <ul>
 
      <li>OverGraduate</li>
 
      <li>UnderGraudate</li>
 
      </ul>
 
  </div>
 
  <div class="col s12 m12 l6">
 
    <div class="row">
 
      <h4>Puxin Wang</h4>
 
      <p>My name is Puxin Wang, an undergraduate student majoring in life science. As a new student in university, I couldn’t do some professional experiment but I can help them translate the experimental report into English. I also do the presentation at the same time.
 
  
  
      </p>
 
      <br/>
 
          <p>ATTRIBUTION:</p>
 
          <div>               
 
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/b/b5/Lzu_china_2015_Humanpractice.png">
 
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/99/Lzu_china_2015_Paperwork.png">
 
          </div>
 
    </div>
 
  </div>
 
    <div class="col s12 m12 l4">
 
    <img src="https://static.igem.org/mediawiki/2015/4/4a/Lzu_china_2015_Lzuwangpuxin.jpg
 
" class="responsive-img avatr">
 
  </div>
 
 
 
</div><!--R18-->
 
       
 
<div class="col s12" style="background-color: rgb(128, 168, 77);">
 
  <div class="col s12 m12 l4">
 
    <img src="https://static.igem.org/mediawiki/2015/c/c3/Lzu_china_2015_Lzuxushuhui.png
 
" class="responsive-img avat">
 
  </div>
 
  <div class="col s12 m12 l6">
 
    <div class="row">
 
      <h4>Shuhui Xu</h4>
 
      <p>It’s me. I’m Shuhui Xu. Love the sunshine and sunny days,and I love the nature. I am one of the guys who operate the MFC devices. Thinking about the tiny creature which creates visual voltage, exciting isn’t it ? I always believe that microorganism has a great potential. So ,let’s make it come true, step by step.
 
  
      </p>
 
        <p>ATTRIBUTION:</p>
 
        <div class="col s12">
 
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/3/30/Lzu_china_2015_Partexamination.png">
 
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/93/Lzu_china_2015_MFC_experiment.png">
 
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/b/b5/Lzu_china_2015_Humanpractice.png">
 
        </div>
 
    </div>
 
  </div>
 
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(128, 168, 77);">
 
      <h1>&nbsp;</h1>
 
      <ul>
 
      <li>OverGraduate</li>
 
      <li>UnderGraudate</li>
 
      </ul>
 
  </div>
 
</div><!--L19-->
 
       
 
<div class="col s12"style="background-color: rgb(44, 138, 239);">
 
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(44, 138, 239);">
 
      <h1>&nbsp;</h1>
 
      <ul>
 
      <li>OverGraduate</li>
 
      <li>UnderGraudate</li>
 
      </ul>
 
  </div>
 
  <div class="col s12 m12 l6">
 
    <div class="row">
 
      <h4>Jinjin Tong</h4>
 
      <p>Hi, I am Jinjin Tong, an optimistic, active and sunny girl. I am a junior student majoring in life science in LanZhou University. In our team, my main task is to operate the Microbial Fuel Cells(MFC), collect data, and analyze data. I've grown a lot during preparing for the iGEM. After this summer vacation, it's going to be my last year of staying in LanZhou University. I hope I will enjoy every minute of every day with my friends and partners.
 
      </p>
 
      <br/>
 
        <p>ATTRIBUTION:</p>
 
        <div>             
 
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/3/30/Lzu_china_2015_Partexamination.png">
 
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/93/Lzu_china_2015_MFC_experiment.png">
 
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/b/b5/Lzu_china_2015_Humanpractice.png">   
 
        </div>
 
    </div>
 
  </div>
 
    <div class="col s12 m12 l4">
 
    <img src="https://static.igem.org/mediawiki/2015/f/f3/Lzu_china_2015_Lzutongjingjing.png
 
" class="responsive-img avatr">
 
  </div>
 
 
 
</div><!--R20-->
 
   
 
<div class="col s12" style="background-color: rgb(159, 115, 76);">
 
  <div class="col s12 m12 l4">
 
    <img src="https://static.igem.org/mediawiki/2015/a/a9/Lzu_china_2015_Lzuyaoningning.jpg
 
" class="responsive-img avat">
 
  </div>
 
  <div class="col s12 m12 l6">
 
    <div class="row">
 
      <h4>Ningning Yao</h4>
 
      <p>I’m Ningning Yao, major in biology from Lanzhou University Cuiying Honors College. It’s my great honor to take part in iGEM as I am enthusiasm in life science. In the past half year, I made porin, constructed k1755003, joined the communication with some universities in Japan and accomplished the previous work of interlab. I also borrowed some experimental material from Jilin University, Nanjing University and Huazhong Agricultural University. Thanks to iGEM, this half year of experience made me feel fulfillment although my work isn’t too much. Wish LZU-2015 can get a good achievement.
 
      </p>
 
        <p>ATTRIBUTION:</p>
 
        <div class="col s12">
 
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/b/b5/Lzu_china_2015_Humanpractice.png">
 
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/93/Lzu_china_2015_MFC_experiment.png">
 
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/99/Lzu_china_2015_Paperwork.png">
 
        </div>
 
    </div>
 
  </div>
 
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(159, 115, 76);">
 
      <h1>&nbsp;</h1>
 
      <ul>
 
      <li>OverGraduate</li>
 
      <li>UnderGraudate</li>
 
      </ul>
 
  </div>
 
</div><!--L21-->
 
       
 
<div class="col s12" style="background-color: rgb(179, 193, 196);">
 
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(179, 193, 196);">
 
      <h1>&nbsp;</h1>
 
      <ul>
 
      <li>OverGraduate</li>
 
      <li>UnderGraudate</li>
 
      </ul>
 
  </div>
 
  <div class="col s12 m12 l6">
 
    <div class="row">
 
      <h4>Xinyu Zhou</h4>
 
      <p>I'm Xinyu Zhou. An outgoing boy who loves anime very much major in biology in Lanzhou University. I like iGEM 'cause it's very creative and we can introduce our new ideas in it. This year in our team I was building part with my partners, also I took part in the human practice and what's more, I'm the lecturer of LZU in the meet up with HokkaidoU, KAIT as well as TokoyoTech in Japan.
 
  
  
      </p>
 
      <br/>
 
      <p>ATTRIBUTION:</p>
 
      <div>               
 
      <img style="width:50px" src="https://static.igem.org/mediawiki/2015/b/b5/Lzu_china_2015_Humanpractice.png">
 
      <img style="width:50px" src="https://static.igem.org/mediawiki/2015/0/09/Lzu_china_2015_Part_construction.png">         
 
      </div>
 
    </div>
 
  </div>
 
    <div class="col s12 m12 l4">
 
    <img src="https://static.igem.org/mediawiki/2015/b/b5/Lzu_china_2015_Lzuzhouxinyu.jpg
 
" class="responsive-img avatr">
 
  </div>
 
 
 
</div><!--R22-->
 
       
 
<div class="col s12" style="background-color: rgb(216, 193, 221);">
 
  <div class="col s12 m12 l4" >
 
    <img src="https://static.igem.org/mediawiki/2015/2/21/Lzu_china_2015_Lzuyangyalan.jpg
 
" class="responsive-img avat">
 
  </div>
 
  <div class="col s12 m12 l6">
 
    <div class="row">
 
      <h4>Yalan Yang</h4>
 
      <p>I’m Yalan Yang, originally working at the wet lab, but now somehow working for human practice. I took part in the publicity, including organizing public activity, registering Twitter and Weibo and finally, dramatizing for a small video. That’s all my work this year.
 
  
      </p>
 
        <p>ATTRIBUTION:</p>
 
        <div class="col s12">
 
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/0/09/Lzu_china_2015_Part_construction.png">
 
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/b/b5/Lzu_china_2015_Humanpractice.png">
 
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/99/Lzu_china_2015_Paperwork.png">
 
        </div>
 
    </div>
 
  </div>
 
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(216, 193, 221);">
 
      <h1>&nbsp;</h1>
 
      <ul>
 
      <li>OverGraduate</li>
 
      <li>UnderGraudate</li>
 
      </ul>
 
  </div>
 
</div><!--L23-->
 
       
 
<div class="col s12" style="background-color: rgb(164, 174, 173);">
 
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(164, 174, 173);" >
 
      <h1>&nbsp;</h1>
 
      <ul>
 
      <li>OverGraduate</li>
 
      <li>UnderGraudate</li>
 
      </ul>
 
  </div>
 
  <div class="col s12 m12 l6">
 
    <div class="row">
 
      <h4>Ning Chai</h4>
 
      <p>I’m Ning Chai, a girl loves nature and is keen on plants. During the time after joining in iGEM, I spared every free time in lab. However, when I have some break time in the lab, I often listen to the pop music or read some English magazine. By the way, Durarara was watched after setting the PCR of RibB in the break time. Now, you must know some of me.
 
  
  
      </p>
 
      <br/>
 
      <p>ATTRIBUTION:</p>
 
      <div>               
 
      <img style="width:50px" src="https://static.igem.org/mediawiki/2015/0/09/Lzu_china_2015_Part_construction.png">
 
      <img style="width:50px" src="https://static.igem.org/mediawiki/2015/b/b5/Lzu_china_2015_Humanpractice.png">
 
      <img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/99/Lzu_china_2015_Paperwork.png">
 
      </div>
 
    </div>
 
  </div>
 
    <div class="col s12 m12 l4">
 
    <img src="https://static.igem.org/mediawiki/2015/b/be/Lzu_china_2015_Lzuchaining.jpg
 
" class="responsive-img right-align avatr">
 
  </div>
 
 
 
</div><!--R24-->
 
       
 
<div class="col s12">
 
  <div class="col s12 m12 l4">
 
    <img src="https://static.igem.org/mediawiki/2015/2/21/Lzu_china_2015_Lzuyangyalan.jpg
 
" class="responsive-img avat">
 
  </div>
 
  <div class="col s12 m12 l6">
 
    <div class="row">
 
      <h4>Qiang Ao</h4>
 
      <p>I’m Yalan Yang, originally working at the wet lab, but now somehow working for human practice. I took part in the publicity, including organizing public activity, registering Twitter and Weibo and finally, dramatizing for a small video. That’s all my work this year.
 
 
      </p>
 
        <p>ATTRIBUTION:</p>
 
        <div class="col s12">
 
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/b/b5/Lzu_china_2015_Humanpractice.png">
 
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/3/35/Lzu_china_2015_Wikicode.png">
 
        </div>
 
    </div>
 
  </div>
 
  <div class="col s12 m12 l2 teamblock">
 
      <h1>&nbsp;</h1>
 
      <ul>
 
      <li>OverGraduate</li>
 
      <li>UnderGraudate</li>
 
      </ul>
 
  </div>
 
</div><!--L23-->
 
 
</div>
 
</div>
 
     
 
    <div id="advis" class="col s12">
 
    <div class="col s12" style="background-color: rgb(212, 213, 217);">
 
  <div class="col s12 m12 l4">
 
    <img src="https://static.igem.org/mediawiki/2015/5/51/LZU_China_Notes_LZUanlizhe.jpg
 
" class="responsive-img avat">
 
  </div>
 
  <div class="col s12 m12 l6">
 
    <div class="row">
 
      <h4>Lizhe An</h4>
 
      <p>Prof. Lizhe An, professor of Life Science College, Vice president of Lanzhou University, went to Chinese Academy of Sciences and Bar-Ilan University to gain his postdoctor. Now his main research direction is botany ecology.
 
 
      </p>
 
 
    </div>
 
  </div>
 
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(212, 213, 217);">
 
      <h1>&nbsp;</h1>
 
      <ul>
 
      <li></li>
 
      <li></li>
 
      </ul>
 
  </div>
 
</div><!--L1-->
 
       
 
    <div class="col s12" style="background-color: rgb(159, 186, 229);">
 
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(159, 186, 229);">
 
      <h1>&nbsp;</h1>
 
      <ul>
 
      <li></li>
 
      <li></li>
 
      </ul>
 
  </div>
 
  <div class="col s12 m12 l6">
 
    <div class="row">
 
      <h4>Huyuan Feng</h4>
 
      <p>Vice dean Prof Huyuan Feng:Prof. Huyuan Feng, Doctor of philosophy graduated in Lanzhou University, as the Vice Dean of School of Life Sciences, Lanzhou University, China will lead this team.  His present research subject is microbio strains living on Dunhuang Mogao Grotto and he devotes all his enthusiam to the study of protection of the historic treasure of the world - Dunhuang Frescoes. He is the ruler of us for the reasons that he is not only a professional expert of our topic but also an intelligent guide of all team.
 
 
      </p>
 
    </div>
 
  </div>
 
    <div class="col s12 m12 l4">
 
    <img src="https://static.igem.org/mediawiki/2015/9/9a/LZU_China_Team_fenghuyuan.jpg
 
" class="responsive-img avatr">
 
  </div>
 
 
    
 
    
</div><!--R2-->
 
   
 
    <div class="col s12" style="background-color: rgb(139, 160, 179);">
 
  <div class="col s12 m12 l4">
 
    <img src="https://static.igem.org/mediawiki/2015/2/27/LZU_China_Notes_LZUyangjinbo.jpg" class="responsive-img avat">
 
  </div>
 
  <div class="col s12 m12 l6">
 
    <div class="row">
 
      <h4>Jinbo Yang</h4>
 
      <p>Prof. Jinbo Yang, Doctor of philosophy graduated in Shanghai Research Institute of Biochemistry is also a member of Cuiying Scholar. He once worked as Postdoctoral Research Fellow, Research Associate Staff, Adjunct Staff and finally became a Consultant Staff in Lerner Research Institute, The Cleveland Clinic Foundation since 2001. He is an expert of tumor treatment and synthetic biology, and in 2013 he was the adviser of LZU-China in iGEM contest.
 
      </p>
 
       
 
    </div>
 
  </div>
 
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(139, 160, 179);">
 
      <h1>&nbsp;</h1>
 
      <ul>
 
      <li></li>
 
      <li></li>
 
      </ul>
 
  </div>
 
</div><!--L3-->
 
       
 
    <div class="col s12" style="background-color: rgb(110, 141, 188);">
 
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(110, 141, 188);">
 
      <h1>&nbsp;</h1>
 
      <ul>
 
      <li></li>
 
      <li></li>
 
      </ul>
 
  </div>
 
  <div class="col s12 m12 l6">
 
    <div class="row">
 
      <h4>Xiangkai Li</h4>
 
      <p>Prof. Xiangkai Li: Prof. Xiangka Li, PhD of microbiology in the University of Oklahoma, is also a member of Cuiying Scholar. He worked as a postdoctor fellow in Baylor College of Medicine in America when finishing his PhD. And after that, he has been a professor in microbiology and doctoral advisor in Lanzhou University till now.
 
      </p>
 
    </div>
 
  </div>
 
    <div class="col s12 m12 l4">
 
    <img src="https://static.igem.org/mediawiki/2015/b/b0/LZU_China_Team_lixiangkai.jpg" class="responsive-img avatr">
 
  </div>
 
 
 
</div><!--R4-->
 
       
 
    <div class="col s12" style="background-color: rgb(191, 233, 255);">
 
  <div class="col s12 m12 l4">
 
    <img src="https://static.igem.org/mediawiki/2015/d/d8/LZU_China_Team_qiuqiang.jpg" class="responsive-img avat">
 
  </div>
 
  <div class="col s12 m12 l6">
 
    <div class="row">
 
      <h4>Qiang Qiu</h4>
 
      <p>Prof. Qiang Qiu, born in may 1982,Weinan ShannXi, he gained Bachelor Degree and Doctor Degree in Life Science College of Lanzhou University in 2004 and 2011. Up to now, he has worked in State Key Laboratory of Grassland Agro-Ecosystem, College of Life Science.
 
      </p>
 
       
 
    </div>
 
  </div>
 
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(191, 233, 255);">
 
      <h1>&nbsp;</h1>
 
      <ul>
 
      <li></li>
 
      <li></li>
 
      </ul>
 
  </div>
 
</div><!--L5-->
 
       
 
    <div class="col s12" style="background-color: rgb(242, 235, 219);">
 
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(242, 235, 219);">
 
      <h1>&nbsp;</h1>
 
      <ul>
 
      <li></li>
 
      <li></li>
 
      </ul>
 
  </div>
 
  <div class="col s12 m12 l6">
 
    <div class="row">
 
      <h4>Yongxing He</h4>
 
      <p>Prof. Yongxing He, bachelor of life science and doctor of molecular and biochemistry in University of Science and Technology of China, went to university of California ,Berkeley in 2011 for his postdoctor fellow in biophysics. When finishing postdoctor, he has been a professor in Lanzhou University till now.
 
      </p>
 
    </div>
 
  </div>
 
    <div class="col s12 m12 l4">
 
    <img src="https://static.igem.org/mediawiki/2015/9/9b/LZU_China_Team_heyongxing.jpg
 
" class="responsive-img avatr">
 
  </div>
 
 
 
</div><!--R6-->
 
       
 
    <div class="col s12">
 
  <div class="col s12 m12 l4">
 
    <img src="https://static.igem.org/mediawiki/2015/c/c5/Lzu_china_2015_Lzuzhangxiao.JPG
 
" class="responsive-img avat">
 
  </div>
 
  <div class="col s12 m12 l6">
 
    <div class="row">
 
      <h4>Zhenmin Ling</h4>
 
      <p>Prof. Qiang Qiu, born in may 1982,Weinan ShannXi, he gained Bachelor Degree and Doctor Degree in Life Science College of Lanzhou University in 2004 and 2011. Up to now, he has worked in State Key Laboratory of Grassland Agro-Ecosystem, College of Life Science.
 
      </p>
 
       
 
    </div>
 
  </div>
 
  <div class="col s12 m12 l2 teamblock">
 
      <h1>&nbsp;</h1>
 
      <ul>
 
      <li></li>
 
      <li></li>
 
      </ul>
 
  </div>
 
</div><!--L5-->
 
       
 
    </div>
 
    <div id="acknow" class="col s12">
 
      <div class="container">
 
          <div class="row">
 
        <h5 class="center-align">ACKNOWLEDGEMENTS</h5>
 
          <p class="center-align"> This project is supported by School of Life Science and Cuiying Honors College, Lanzhou University, MOST international cooperation funds, 2014DFA91340, Gansu provincial international cooperation funds, 1304WCGA176, Majorbio Cooperation and Takara China.</p>
 
        </div>
 
        <h6 class="center-align">Lanzhou University</h6>
 
        <p class="center-align">Lanzhou University has been the best university in the northwestern region of China, ranking as one of the top 30 among over 1,500 universities across China. It is one of the key universities administered directly by the Ministry of Education.</p>
 
        <h6 class="center-align">School of Life Sciences</h6>
 
        <p class="center-align">The School of Life Sciences at Lanzhou University has a long history. It originated as the Department of Botany and the Department of Zoology of National Lanzhou University, which were founded in 1946 as the first departments of life sciences in western China. After several generations' efforts in over sixty years, the School of Life Science is in a good development trend in many aspects, such as faculty and staff, discipline construction, teaching and researching, and local service. Basing its scientific research on the cold and arid environment in western China, the faculty has made great achievements. The School of Life Sciences is active in international collaboration in order to create an open and supportive academic atmosphere, extend academic horizons, and improve the faculty and students' ability in research and creativity.</p>
 
        <h6 class="center-align">Cuiying Honors College</h6>
 
        <p class="center-align">Cuiying Honors College (CHC) was founded in August 2010 within Lanzhou University as part of China's Top-Notch Undergraduate Training Program. The Ministry of Education and the Ministry of Finance are investing in this new pilot scheme in order to develop future leaders with exceptional talent in the core disciplines. Every year this program only admits the best 1000 students from the 6 million new undergraduates nationwide. Lanzhou University has been selected as one of 19 institutions implementing this national program.</p>
 
      </div>
 
        </div>
 
  </div>
 
 
</div>
 
</div>
  
  
 
+
<footer class="page-footer teal">
 
+
 
+
 
+
 
+
 
+
  <footer class="page-footer teal">
+
 
     <div class="container">
 
     <div class="container">
 
       <div class="row">
 
       <div class="row">
 
       <div style="height:20px;width:100%;float:left">
 
       <div style="height:20px;width:100%;float:left">
 
    
 
    
</div>
+
      </div>
 
         <div class="col l3 s12">
 
         <div class="col l3 s12">
 
           <img src="https://static.igem.org/mediawiki/2015/c/c3/Lzu_china_2015_Sch_icon1.png" alt="" class="circle responsive-img">  
 
           <img src="https://static.igem.org/mediawiki/2015/c/c3/Lzu_china_2015_Sch_icon1.png" alt="" class="circle responsive-img">  
Line 1,100: Line 222:
 
       </div>
 
       </div>
 
     </div>
 
     </div>
  </footer>
+
</footer>
  
  
 
   <!--  Scripts-->
 
   <!--  Scripts-->
 +
 
   <script src="https://code.jquery.com/jquery-2.1.1.min.js"></script>
 
   <script src="https://code.jquery.com/jquery-2.1.1.min.js"></script>
   <script src="https://2015.igem.org/Team:LZU-China/chz_materializejs?action=raw&ctype=text/javascript"></script>
+
   <script src="https://2015.igem.org/Team:LZU-China/chz_materialize?action=raw&ctype=text/javascript"></script>
 
   <script src="https://2015.igem.org/Team:LZU-China/chz_init?action=raw&ctype=text/javascript"></script>
 
   <script src="https://2015.igem.org/Team:LZU-China/chz_init?action=raw&ctype=text/javascript"></script>
 
   <script src="https://2015.igem.org/Team:LZU-China/chz_headroom?action=raw&ctype=text/javascript"></script>
 
   <script src="https://2015.igem.org/Team:LZU-China/chz_headroom?action=raw&ctype=text/javascript"></script>
  
<script>
 
    var myElement = document.querySelector("header");
 
  
var headroom  = new Headroom(myElement);
+
  <!--<script src="js/jquery.grumble.min.js"></script>-->
// 初始化
+
 
headroom.init();
+
  <!--<script src="js/Aristochart.js"></script>-->
     </script>
+
  <script src="https://2015.igem.org/Team:LZU-China/chz_pin?action=raw&ctype=text/javascript"></script>
 +
 
 +
 
 +
 
 +
   
 +
   
 +
 
 +
<!--Pin something-->
 +
  <script>
 +
      $(".pinnedx").pin({
 +
          containerSelector: ".contain-box"
 +
     })
 +
  </script>
 +
 
 +
 
 
      
 
      
 
      
 
      
 
      
 
      
 +
 
 +
 +
<script>
 +
 +
var myElement = document.querySelector("header");
 +
 +
var headroom  = new Headroom(myElement);
 +
 +
headroom.init();
 +
</script>
 +
 
   </body>
 
   </body>
 
</html>
 
</html>

Revision as of 19:07, 17 September 2015

Team:LZU 2015

 

The collaboration with TJU

 

We started our contact with team TJU from the very beginning of the summer. Since both teams are working on the MFC system, we have shared a lot of experience on it, including the MFC device setup, medium condition control and part characterization. Additionally, we had lots of online and offline discussion with them.


In the middle of the project, we came to Key Laboratory of Systems Bioengineering (Tianjin University) to have a project seminar with them. During the discussion, we knew that they have constructed a lactate producing part named ldhE. However, their characterization of this part was faced with some problem. To be specific, engineered E.coli strain bearing the ldhE part showed little difference with the wild type. (shown as follows)

Fig.1 The production of lactate in MG1665-WT. The cells were cultured in 37C for 15h and the lactate concentration was measured by HPLC.


For the lactate producing system, we have helped team TJU to optimize and characterize the ldhE part. From their introduction, we know that LDH is an enzyme catalyzes the conversion of pyruvate to lactate with NADH serving as the coenzyme. Initially, they intended to introduce high-yield u L-(+)-lactate dehydrogenase gene (ldhA) from Lactobacillus, which could produce a relatively larger amount of L-lactate for Shewanella. Consequently, we decided to focus on the assistant method to help them resolve the problem.

After some consultant, we noticed that in the wild type E. coli, LDH reaction is not as competitive as the reaction through PFL, which might result in the ineffectiveness of ldhE.[1] Therefore, knockout of the PFL related genes will contribute to redistribute the metabolic flux. In addition, we searched in the part registry of IGEM and found the part BBa_K341458 and part BBa_K341002, which revealed a more effective and easy-to-use method for gene knockout.


In the system we searched, the cell is first transformed with a helper plasmid harboring genes encoding the λ-Red enzymes, I-SceI endonuclease, and RecA. λ-Red enzymes expressed from the helper plasmids are used to recombine a small ‘landing pad’, a tetracycline resistance gene (tetA) flanked by I-SceI recognition sites and landing pad regions, into the desired location in the chromosome. After tetracycline selection for successful landing pad integrants, the cell is transformed with a donor plasmid carrying the desired insertion fragment; this fragment is excised by I-SceI and incorporated into the landing pad via recombination at the landing pad regions.[2] We think this more effective and easy method would help them overcome the problem. So, we recommended TJU to adopt the two-step λ-Red method to knock out the pflB gene.


After their reconstruction of the engineered strain, a more visible contrast was shown as below.

Fig.2 The production of lactate of the MG1655ΔpflB. The cells are cultured in 37C. (Data from TJU)


In order to prove the cell can work normally, we tested the knock-out cell’s field of lactate in our lab. The result is shown in the figure below.

Fig.3 The production of lactate of the MG1655ΔpflB. The cells are cultured in 37C for 26h. (data from LZU-China)


References

[1]Zhu J, Shimizu K, Zhu J, et al. Effect of a single-gene knockout on the metabolic regulation in Escherichia coli for D-lactate production under microaerobic condition[J]. Metabolic Engineering, 2005, 7(2):104–115.

[2]Thomas E, Kuhlman, Edward C, Cox. Site-specific chromosomal integration of large synthetic constructs.[J]. Nucleic Acids Research, 2010, 38(38).