Difference between revisions of "Team:TU Darmstadt/Notebook/sec1/K1602025"

(Blanked the page)
Line 1: Line 1:
 +
{{:Team:TU_Darmstadt/Templates/CSS}}
 +
<h3> K1602028 - B0034-HistidinTag-Green fluorescent protein-SilicaTag4 (B0034-His-GFP-Si4)</h3>
  
 +
 +
<html><div class="contentSection">
 +
 +
<p align="justify">
 +
<a href=" https://2015.igem.org/Team:TU_Darmstadt/Notebook/sec1/K1602027 " title="Opens internal link in current window" class="internal link">K1602027</a> was digested with <em>Xba</em>I and <em>Pst</em>I and ligated into B0034-pSB1A2 and transformed into <em>E. coli</em> Top 10. Colonies were screened by colony PCR with oligonucleotides VF2 and VR. Positive colonies were inoculated and the plasmids were extracted (Figure 1). </p>
 +
 +
 +
 +
 +
 +
<figure class="leftFig"  style="width:30%">
 +
<img src="https://static.igem.org/mediawiki/2015/a/ac/Figure_3-_Colony_PCR_of_B0034-Prefix-His-Tag-GFP-Si4.png" width=50% height=50%>
 +
<figcaption><br><b>Figure 1</b> Colony PCR of B0034-Prefix-His-Tag-GFP-Si4 (1). Colony 1 contained the insert of around  1.1 kbp. DNA marker: 2-Log DNA Ladder.
 +
</figcaption>
 +
</figure>
 +
 +
</div></html>

Revision as of 20:34, 17 September 2015

K1602028 - B0034-HistidinTag-Green fluorescent protein-SilicaTag4 (B0034-His-GFP-Si4)


K1602027 was digested with XbaI and PstI and ligated into B0034-pSB1A2 and transformed into E. coli Top 10. Colonies were screened by colony PCR with oligonucleotides VF2 and VR. Positive colonies were inoculated and the plasmids were extracted (Figure 1).


Figure 1 Colony PCR of B0034-Prefix-His-Tag-GFP-Si4 (1). Colony 1 contained the insert of around 1.1 kbp. DNA marker: 2-Log DNA Ladder.