Difference between revisions of "Team:Kent/Notebook"

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     <th> M </th> <th> T </th> <th> W </th><th> T </th><th> F </th><th> S </th><th> S </th>
 
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     <th> 1</th><th> 2 </th><th> 3 </th><th> 4 </th><th> 5 </th><th> 6 </th><th> 7 </th>
 
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     <th> 8</th><th> 9 </th><th> 10 </th><th> 11 </th><th> 12 </th><th> 13 </th><th> 14 </th>
 
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     <th> <a href="#day1">22</a> </th><th><a href="#day2"> 23</a> </th><th><a href="#day3"> 24</a> </th><th><a href="#day4"> 25</a> </th><th><a href="#day5"> 26</a> </th><th> 27 </th><th> 28 </th>
 
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     <th> 29 </th><th> 30 </th><th> </th><th> </th><th> </th><th> </th><th> </th>
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     <th><a href="#day6"> 29</a> </th><th> 30 </th><th> </th><th> </th><th> </th><th> </th><th> </th>
 
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</table>
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<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
 
<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
 
   
 
   
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<h3> Contents </h3>
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<a name="day1"></a><h4> Day 1 Wet lab <i>22/06/15</i> </h4>
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<a href="#week1">Week 1</a> <br>
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<a href="#week2">Week 2</a>
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<a name="week1"></a><h3> Week 1 </h3>
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<h4> Day 1 Wet lab <i>22/06/15</i> </h4>
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<p><li> Made LB plates </li>
 
<p><li> Made LB plates </li>
 
       <li> Made LB broth </li>
 
       <li> Made LB broth </li>
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</p>
 
</p>
  
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<h4> Day 2 Wet lab <i>23/06/15</i> </h4>
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<a name="day2"></a><h4> Day 2 Wet lab <i>23/06/15</i> </h4>
 
<p><li> Set up Top10 overnight, VS45 overnight, US348 overnight </li>
 
<p><li> Set up Top10 overnight, VS45 overnight, US348 overnight </li>
 
</p>
 
</p>
  
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<h4> Day 3 Wet lab <i>24/06/15</i></h4>
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<a name="day3"></a><h4> Day 3 Wet lab <i>24/06/15</i></h4>
 
<p><li> Filter sterilise the buffer</li>
 
<p><li> Filter sterilise the buffer</li>
 
       <li> 250ml no antibiotic broth, put top10 cells in</li>
 
       <li> 250ml no antibiotic broth, put top10 cells in</li>
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</p>
 
</p>
  
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<h4> Day 4 Wet lab <i> 25/06/15 </i> </h4>
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<a name="day4"></a><h4> Day 4 Wet lab <i> 25/06/15 </i> </h4>
 
<p> <li> Transformation </li>
 
<p> <li> Transformation </li>
 
       <li> Prepare SBC media </li>
 
       <li> Prepare SBC media </li>
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</p>
 
</p>
  
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<h4> Day 5 Wet lab <i> 26/06/15 </i> </h4>
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<a name="day5"></a><h4> Day 5 Wet lab <i> 26/06/15 </i> </h4>
 
<p><li> Calculated competent cell efficiency </li>
 
<p><li> Calculated competent cell efficiency </li>
 
       <li> Agarose gel formation </li>
 
       <li> Agarose gel formation </li>
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</p>
 
</p>
  
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<a name="week2"></a><h3> Week 2 </h3>
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<a name="day6"></a><h4> Day 6 Wet lab <i> 29/06/15 </i> </h4>
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<h4> Day 6 Wet lab <i> 29/06/15 </i> </h4>
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<p></li> Transforming linear PSBC13 </li>
 
<p></li> Transforming linear PSBC13 </li>
 
</p>
 
</p>

Revision as of 09:50, 30 June 2015


iGEM Kent 2015

Notebook

June
M T W T F S S
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
M T W T F S S
1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 2930 31
August
M T W T F S S
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 2627 28 29 30
31
September
M T W T F S S
1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30


















Day 1 Wet lab 22/06/15

  • Made LB plates
  • Made LB broth
  • TIPS autoclaved

    • Day 2 Wet lab 23/06/15

  • Set up Top10 overnight, VS45 overnight, US348 overnight

    • Day 3 Wet lab 24/06/15

  • Filter sterilise the buffer
  • 250ml no antibiotic broth, put top10 cells in
  • Incubate until optical density is 0.6
  • Miniprep PSB1C3 plasmid

    • Day 4 Wet lab 25/06/15

  • Transformation
  • Prepare SBC media
  • Digest of PSB1c3 from MS348

    • Day 5 Wet lab 26/06/15

  • Calculated competent cell efficiency
  • Agarose gel formation
  • Gel electrophoresis

    • Day 6 Wet lab 29/06/15

    Transforming linear PSBC13